Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage

  • Authors:
    • Wan‑Zong Wang
    • Xiao‑Dong Yao
    • Xiao‑Jin Huang
    • Jin‑Quan Li
    • Hao Xu
  • View Affiliations

  • Published online on: June 22, 2015     https://doi.org/10.3892/etm.2015.2584
  • Pages: 995-1002
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to investigate the effect of a three-dimensional (3D) culture system of sodium alginate gel on the directional differentiation induction of bone marrow‑derived mesenchymal stem cells (BMSCs) into chondrocytes, as well as the in vitro gene transfection technique. The biological characteristics of the passage and proliferation of rabbit BMSCs were investigated under conditions of in vitro monolayer and 3D culture of sodium alginate gel. Transforming growth factor (TGF)‑β1 gene recombinant adenoviral cosmid vectors and the recombinant adenoviral vector Ad.TGF‑β1 were constructed, and the effect of Ad.TGF‑β1 transfection on the differentiation of BMSCs into chondrocytes was investigated. The whole bone marrow rinsing method was used to obtain, separate and purify the rabbit BMSCs, and the in vitro monolayer and 3D culture of sodium alginate gel were thus successfully and stably established. A safe, stable and efficient method of constructing Ad.TGF‑β1 TGF‑β1 gene recombinant adenoviral vectors was established. Following TGF‑β1 transfection, BMSCs were able to continuously secrete significantly increased amounts of specific extracellular matrix components of chondrocytes, such as collagen Ⅱ and proteoglycans. Furthermore, the effects in the post‑gene transfection 3D culture group were found to be enhanced compared with those in the monolayer culture group. In conclusion, the 3D culture system of sodium alginate gel and in vitro gene transfection exhibited significant inductive effects on differentiation, which could be used to promote BMSCs to differentiate into chondrocytes.
View Figures
View References

Related Articles

Journal Cover

September-2015
Volume 10 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang WZ, Yao XD, Huang XJ, Li JQ and Xu H: Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage. Exp Ther Med 10: 995-1002, 2015
APA
Wang, W., Yao, X., Huang, X., Li, J., & Xu, H. (2015). Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage. Experimental and Therapeutic Medicine, 10, 995-1002. https://doi.org/10.3892/etm.2015.2584
MLA
Wang, W., Yao, X., Huang, X., Li, J., Xu, H."Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage". Experimental and Therapeutic Medicine 10.3 (2015): 995-1002.
Chicago
Wang, W., Yao, X., Huang, X., Li, J., Xu, H."Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage". Experimental and Therapeutic Medicine 10, no. 3 (2015): 995-1002. https://doi.org/10.3892/etm.2015.2584