search button

TGF-β1 induces apoptosis of bone marrow-derived mesenchymal stem cells via regulation of mitochondrial reactive oxygen species production

  • Authors:
    • Fenxi Zhang
    • Tongming Ren
    • Junfang Wu

  • View Affiliations

  • Published online on: June 23, 2015     https://doi.org/10.3892/etm.2015.2590
  • Pages: 1224-1228
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) are the most promising seed cells in regenerative medicine. Our previous study demonstrated that transforming growth factor (TGF)-β1 induced BMSC senescence in vitro. Whether TGF‑β1 affects the apoptosis of BMSCs has not been examined; therefore the aim of the present study was to investigate this effect. BMSCs were isolated from mouse bone marrow, and the third‑passage cells were exposed to 0, 10 and 20 ng/ml TGF‑β1 for 24 h. Cell proliferation was measured by MTT assay; apoptosis was assessed using DAPI staining; and the apoptotic signals Annexin V, B‑cell lymphoma (Bcl)‑2 and Bcl‑2‑associated X protein (Bax) were measured using western blotting. Mitochondrial reactive oxygen species (ROS) were measured by flow cytometry following staining with MitoSOX™ Red mitochondrial superoxide indicator. The MTT assay showed that 10 and 20 ng/ml TGF‑β1 inhibited BMSC proliferation. DAPI staining demonstrated that 10 and 20 ng/ml TGF‑β1 promoted BMSC apoptosis, which was further confirmed by a western blotting assay showing a significant increase in the pro‑apoptotic signals Annexin V and Bax but a decrease in the anti‑apoptotic signal Bcl‑2. It was also found that TGF‑β1 markedly increased the mitochondrial ROS levels in BMSCs. It is well known that mitochondrial ROS are strong stimulators of cell apoptosis. These findings indicate that TGF-β1 can induce BMSC apoptosis, and the mechanism may involve mitochondrial ROS generation.
View Figures
View References

Related Articles

Journal Cover

September-2015
Volume 10 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 2.7
  • Ranked Medicine Research and Experimental
    (total number of cites)
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang F, Ren T and Wu J: TGF-β1 induces apoptosis of bone marrow-derived mesenchymal stem cells via regulation of mitochondrial reactive oxygen species production. Exp Ther Med 10: 1224-1228, 2015
APA
Zhang, F., Ren, T., & Wu, J. (2015). TGF-β1 induces apoptosis of bone marrow-derived mesenchymal stem cells via regulation of mitochondrial reactive oxygen species production. Experimental and Therapeutic Medicine, 10, 1224-1228. https://doi.org/10.3892/etm.2015.2590
MLA
Zhang, F., Ren, T., Wu, J."TGF-β1 induces apoptosis of bone marrow-derived mesenchymal stem cells via regulation of mitochondrial reactive oxygen species production". Experimental and Therapeutic Medicine 10.3 (2015): 1224-1228.
Chicago
Zhang, F., Ren, T., Wu, J."TGF-β1 induces apoptosis of bone marrow-derived mesenchymal stem cells via regulation of mitochondrial reactive oxygen species production". Experimental and Therapeutic Medicine 10, no. 3 (2015): 1224-1228. https://doi.org/10.3892/etm.2015.2590