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Effects of autologous bone marrow‑derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats

  • Authors:
    • Lingyun Bi
    • Guohong Wang
    • Dasheng Yang
    • Shujun Li
    • Bin Liang
    • Ziming Han
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Laboratory of Physiology, Xinxiang Medical University, Xinxiang, Henan 453000, P.R. China
    Copyright: © Bi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 851-856
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    Published online on: June 24, 2015
       https://doi.org/10.3892/etm.2015.2592
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Abstract

The aim of this study was to investigate the effects of stem cell factor (SCF) and granulocyte colony‑stimulating factor (G‑CSF) on bone marrow‑derived stem cell (BMSC) mobilization in rat models of renal ischemia/reperfusion (I/R) injury. In addition, the effects of SCF and G‑CSF on cellular apoptosis were explored in order to determine the protective mechanism of the two factors against renal I/R injury. A unilateral renal I/R injury model was established for the model and treatment groups. The treatment and treatment control groups were subcutaneously injected with SCF (200 µg/kg/day) and G‑CSF (50 µg/kg/day) 24 h after the establishment of the model for five consecutive days. The total number of leukocytes in the peripheral blood and the cellular percentages of cluster of differentiation (CD)34+, renal CD34+ and apoptotic cells were detected. The total number of leukocytes in the peripheral blood and the percentages of CD34+ cells in the treatment and treatment control groups reached maximum levels on the fifth postoperative day and were significantly higher than those in the normal control and model groups. The number of renal CD34+ cells in the treatment group was significantly increased compared with that in the treatment control and model groups. The apoptotic indices (AIs) of the model and treatment groups were higher than those of the normal control and treatment control groups. The AI of the model group was significantly higher than that of the treatment group. In conclusion, the combined application of SCF and G‑CSF can mobilize sufficient numbers of BMSCs and cause cellular ‘homing’ to the injured site, thus inhibiting apoptosis and promoting the repair of renal tubular injury.
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Copy and paste a formatted citation
Spandidos Publications style
Bi L, Wang G, Yang D, Li S, Liang B and Han Z: Effects of autologous bone marrow‑derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats. Exp Ther Med 10: 851-856, 2015.
APA
Bi, L., Wang, G., Yang, D., Li, S., Liang, B., & Han, Z. (2015). Effects of autologous bone marrow‑derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats. Experimental and Therapeutic Medicine, 10, 851-856. https://doi.org/10.3892/etm.2015.2592
MLA
Bi, L., Wang, G., Yang, D., Li, S., Liang, B., Han, Z."Effects of autologous bone marrow‑derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats". Experimental and Therapeutic Medicine 10.3 (2015): 851-856.
Chicago
Bi, L., Wang, G., Yang, D., Li, S., Liang, B., Han, Z."Effects of autologous bone marrow‑derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats". Experimental and Therapeutic Medicine 10, no. 3 (2015): 851-856. https://doi.org/10.3892/etm.2015.2592
Copy and paste a formatted citation
x
Spandidos Publications style
Bi L, Wang G, Yang D, Li S, Liang B and Han Z: Effects of autologous bone marrow‑derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats. Exp Ther Med 10: 851-856, 2015.
APA
Bi, L., Wang, G., Yang, D., Li, S., Liang, B., & Han, Z. (2015). Effects of autologous bone marrow‑derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats. Experimental and Therapeutic Medicine, 10, 851-856. https://doi.org/10.3892/etm.2015.2592
MLA
Bi, L., Wang, G., Yang, D., Li, S., Liang, B., Han, Z."Effects of autologous bone marrow‑derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats". Experimental and Therapeutic Medicine 10.3 (2015): 851-856.
Chicago
Bi, L., Wang, G., Yang, D., Li, S., Liang, B., Han, Z."Effects of autologous bone marrow‑derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats". Experimental and Therapeutic Medicine 10, no. 3 (2015): 851-856. https://doi.org/10.3892/etm.2015.2592
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