Differences in 23S ribosomal RNA mutations between wild-type and mutant macrolide-resistant Chlamydia trachomatis isolates

Jiang,Yong; Zhu,Hui; Yang,Li‑Na; Liu,Yuan‑Jun; Hou,Shu‑Ping; Qi,Man‑Li; Liu,Quan‑Zhong

doi:10.3892/etm.2015.2595

September-2015 Volume 10 Issue 3

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September-2015 Volume 10 Issue 3

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Article

Differences in 23S ribosomal RNA mutations between wild-type and mutant macrolide-resistant Chlamydia trachomatis isolates

Authors:
- Yong Jiang
- Hui Zhu
- Li‑Na Yang
- Yuan‑Jun Liu
- Shu‑Ping Hou
- Man‑Li Qi
- Quan‑Zhong Liu
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Affiliations: Department of Dermatology, The Second Hospital of Tianjin Medical University, Heping, Tianjin 300052, P.R. China, Department of Dermatology, General Hospital of Tianjin Medical University, Heping, Tianjin 300052, P.R. China
Pages: 1189-1193
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Published online on: June 24, 2015

https://doi.org/10.3892/etm.2015.2595
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Abstract

The aim of the present study was to determine the in vitro susceptibility of wild-type and mutant clinical isolates of Chlamydia (C.) trachomatis strains to erythromycin, azithromycin and josamycin, and to identify the resistance-conferring 23S ribosomal (r)RNA mutations in the isolates. The wild‑type resistant isolates were defined as those with minimum inhibitory concentration values above the tissue concentration of the antibiotic in the urogenital system. Furthermore, all resistant C. trachomatis isolates were exposed to sub‑inhibitory concentrations of macrolides, and 13 resistant mutants were selected following serial passages. Among the 8 wild‑type isolates that were resistant to erythromycin, 3 isolates had a mutation at T2611C in the 23S rRNA gene while the others did not show any 23S rRNA mutations. The selected mutant isolates showed a 4‑ to 16‑fold reduction in in vitro sensitivities. With regard to the mutant strains, the T2611C mutation was found in 10 isolates, A2057G mutation in 6 isolates, and A2059G mutation in 1 isolate. Thus, the macrolide‑resistant isolates of the wild‑type strain had different mutations from those selected by exposure to sub‑inhibitory concentrations of macrolides. Also, since 23S rRNA mutations were not identified in certain isolates, it was considered that other molecular mechanisms may also be responsible for the macrolide resistance of C. trachomatis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Differences in 23S ribosomal RNA mutations between wild-type and mutant macrolide-resistant Chlamydia trachomatis isolates

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