Wogonin inhibits osteoclast differentiation by inhibiting NFATc1 translocation into the nucleus

  • Authors:
    • Xiaolin Geng
    • Libin Yang
    • Chao Zhang
    • Hua Qin
    • Qiudong Liang
  • View Affiliations

  • Published online on: July 3, 2015     https://doi.org/10.3892/etm.2015.2615
  • Pages: 1066-1070
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Abstract

The aim of the present study was to identify a natural product with the ability to inhibit nuclear factor of activated T cells c1 (NFATc1) translocation from the cytoplasm to the nucleus by high‑throughput screening, and to investigate the effect of the natural product upon osteoclast differentiation and its underlying mechanism. An NFATc1 antagonist redistribution assay was performed in U2OS‑NFATc1 cells against a natural product library, and Wogonin was found to have the ability to inhibit the NFATc1 translocation from the cytoplasm to the nucleus. The effect of Wogonin on NFATc1 transcription activation was further determined by luciferase assay. An osteoclast differentiation assay was executed to evaluate the effect of Wogonin on osteoclast differentiation. The effect of Wogonin upon the vital genes in osteoclast differentiation was investigated using fluorescent quantitative polymerase chain reaction analysis. The natural product Wogonin significantly inhibited the translocation of NFATc1 from the cytoplasm to the nucleus and its transcriptional activation activity. Wogonin also significantly inhibited osteoclast differentiation and decreased the transcription of osteoclast‑associated immunoglobulin‑like receptor, tartrate‑resistant acid phosphatase and calcitonin receptor. In conclusion, the natural product Wogonin inhibited osteoclast differentiation through the inhibition of NFATc1 translocation from the cytoplasm to the nucleus, and thus the downregulation of genes associated with osteoclast differentiation, which marked Wogonin as a potential treatment for osteoporosis.
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September-2015
Volume 10 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Geng X, Yang L, Zhang C, Qin H and Liang Q: Wogonin inhibits osteoclast differentiation by inhibiting NFATc1 translocation into the nucleus. Exp Ther Med 10: 1066-1070, 2015
APA
Geng, X., Yang, L., Zhang, C., Qin, H., & Liang, Q. (2015). Wogonin inhibits osteoclast differentiation by inhibiting NFATc1 translocation into the nucleus. Experimental and Therapeutic Medicine, 10, 1066-1070. https://doi.org/10.3892/etm.2015.2615
MLA
Geng, X., Yang, L., Zhang, C., Qin, H., Liang, Q."Wogonin inhibits osteoclast differentiation by inhibiting NFATc1 translocation into the nucleus". Experimental and Therapeutic Medicine 10.3 (2015): 1066-1070.
Chicago
Geng, X., Yang, L., Zhang, C., Qin, H., Liang, Q."Wogonin inhibits osteoclast differentiation by inhibiting NFATc1 translocation into the nucleus". Experimental and Therapeutic Medicine 10, no. 3 (2015): 1066-1070. https://doi.org/10.3892/etm.2015.2615