Effect of non‑genomic actions of thyroid hormones on the anaesthetic effect of propofol

Wang,Yang; Ma,Ketao; Li,Li; Liu,Yanhui; Si,Junqiang; Wan,Yu

doi:10.3892/etm.2015.2624

Effect of non‑genomic actions of thyroid hormones on the anaesthetic effect of propofol

Authors:
- Yang Wang
- Ketao Ma
- Li Li
- Yanhui Liu
- Junqiang Si
- Yu Wan
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Affiliations: Department of Physiology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China, Electrophysiological Laboratory, Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi, Xinjiang 832002, P.R. China, Electrophysiological Laboratory, Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi, Xinjiang 832002, P.R. China
Published online on: July 7, 2015 https://doi.org/10.3892/etm.2015.2624
Pages: 959-965
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hyperthyroidism is a common disease of the endocrine system and it is known that additional propofol anaesthesia is required during surgery for patients with hyperthyroidism compared with those with normal thyroid function. The aim of the present study was to determine the mechanism through which thyroid hormones (THs) inhibit the effect of propofol anaesthesia. Immunofluorescence techniques were used to verify the difference between the expression quantities of γ‑aminobutyric acid type A (GABAA) receptor subunits α2 and β2 in the dorsal root ganglions (DRGs) of rats with hyperthyroidism and those in normal rats. Perforated patch clamp recordings in the whole‑cell mode were performed to detect the GABA‑activated current in acutely isolated rat DRG neurons from rats with hyperthyroidism and normal rats. This method was also used to evaluate the change in the GABA‑activated currents following the pre‑perfusion of propofol with and without 3,3',5‑L‑triiodothyronine (T3). Compared with normal rats, rats with hyperthyroidism expressed same quantities of GABAA receptor α2 and β2 subunits in DRGs. In addition, no difference in GABA‑activated currents in the acutely isolated DRG neurons from the two types of rat was observed (P>0.05). T3 inhibits or minimises the augmentation effect of propofol on the GABA‑activated currents (P<0.05). The inhibitory effect of T3 on propofol was minimised by increasing the propofol concentration (P<0.05). The inhibitory effect of T3 on the anaesthetic effect of propofol is achieved through the inhibition of the function of GABAA receptors through the non‑genomic actions of the THs, rather than by changing the number of GABAA receptors. This inhibitory effect can be mitigated by increasing the propofol concentration. In conclusion, rats with hyperthyroidism require a larger dose of propofol to induce anaesthesia since the non‑genomic actions of THs suppress GABA receptors, which in turn inhibits the anaesthetic action of propofol.

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1	Greenhill C: Thyroid function: Hyperthyroidism - psychiatric issues. Nat Rev Endocrinol. 10:652014. View Article : Google Scholar
2	Brandt F, Thvilum M, Almind D, et al: Hyperthyroidism and psychiatric morbidity: Evidence from a Danish nationwide register study. Eur J Endocrinol. 170:341–348. 2014. View Article : Google Scholar : PubMed/NCBI
3	Ertek S and Cicero AF: Hyperthyroidism and cardiovascular complications: A narrative review on the basis of pathophysiology. Arch Med Sci. 9:944–952. 2013. View Article : Google Scholar : PubMed/NCBI
4	Bajwa SJ and Sehgal V: Anesthesia and thyroid surgery: The never ending challenges. Indian J Endocrinol Metab. 17:228–234. 2013. View Article : Google Scholar : PubMed/NCBI
5	Tsubokawa T, Yamamoto K and Kobayashi T: Propofol clearance and distribution volume increase in patients with hyperthyroidism. Anesth Analg. 87:195–199. 1998. View Article : Google Scholar : PubMed/NCBI
6	Wang YG, Song XJ, Feng SW, Ge YL, Yang JJ and He LL: Hyperthyroidism patients have shorter onset and duration time of rocuronium than euthyroidism patients. J Pharm Pharm Sci. 10:53–60. 2007.PubMed/NCBI
7	Kumar VV and Kaimar P: Subclinical hypothyroidism: A cause for delayed recovery from anaesthesia? Indian. J Anaesth. 55:433–434. 2011.
8	Trapani G, Altomare C, Liso G, Sanna E and Biggio G: Propofol in anesthesia. Mechanism of action, structure-activity relationships, and drug delivery. Curr Med Chem. 7:249–271. 2000. View Article : Google Scholar : PubMed/NCBI
9	Hara M, Kai Y and Ikemoto Y: Enhancement by propofol of the gamma-aminobutyric acid A response in dissociated hippocampal pyramidal neurons of the rat. Anesthesiology. 81:988–994. 1994. View Article : Google Scholar : PubMed/NCBI
10	Olsen RW and Sieghart W: International Union of Pharmacology. LXX. Subtypes of gamma-aminobutyric acid (A) receptors: classification on the basis of subunit composition, pharmacology, and function. Update. Pharmacol Rev. 60:243–260. 2008. View Article : Google Scholar
11	Duveau V, Laustela S, Barth L, et al: Spatiotemporal specificity of GABA_A receptor-mediated regulation of adult hippocampal neurogenesis. Eur J Neurosci. 34:362–373. 2011. View Article : Google Scholar : PubMed/NCBI
12	Olsen RW and Sieghart W: GABA A receptors: Subtypes provide diversity of function and pharmacology. Neuropharmacology. 56:141–148. 2009. View Article : Google Scholar : PubMed/NCBI
13	Gupta MK and Misra K: Atom-based 3D-QSAR, molecular docking and molecular dynamics simulation assessment of inhibitors for thyroid hormone receptor α and β. J Mol Model. 20:22862014. View Article : Google Scholar : PubMed/NCBI
14	Puia G and Losi G: Thyroid hormones modulate GABA(A) receptor-mediated currents in hippocampal neurons. Neuropharmacology. 60:1254–1261. 2011. View Article : Google Scholar : PubMed/NCBI
15	Li M, Iismaa SE, Naqvi N, Nicks A, Husain A and Graham RM: Thyroid hormone action in postnatal heart development. Stem Cell Res (Amst). 13:582–591. 2014. View Article : Google Scholar
16	Wirth EK, Schweizer U and Köhrle J: Transport of thyroid hormone in brain. Front Endocrinol (Lausanne). 5:982014.PubMed/NCBI
17	Morte B and Bernal J: Thyroid hormone action: Astrocyte-neuron communication. Front Endocrinol (Lausanne). 5:822014.PubMed/NCBI
18	Bhumika S and Darras VM: Role of thyroid hormones in different aspects of nervous system regeneration in vertebrates. Gen Comp Endocrinol. 203:86–94. 2014. View Article : Google Scholar : PubMed/NCBI
19	Davis PJ, Tillmann HC, Davis FB and Wehling M: Comparison of the mechanisms of nongenomic actions of thyroid hormone and steroid hormones. J Endocrinol Invest. 25:377–388. 2002. View Article : Google Scholar : PubMed/NCBI
20	Bergh JJ, Lin HY, Lansing L, et al: Integrin alphaVbeta3 contains a cell surface receptor site for thyroid hormone that is linked to activation of mitogen-activated protein kinase and induction of angiogenesis. Endocrinology. 146:2864–2871. 2005. View Article : Google Scholar : PubMed/NCBI
21	Davis PJ, Davis FB and Cody V: Membrane receptors mediating thyroid hormone action. Trends Endocrinol Metab. 16:429–435. 2005. View Article : Google Scholar : PubMed/NCBI
22	Lin HY, Sun M, Tang HY, et al: L-Thyroxine vs. 3,5,3′-triiodo-L-thyronine and cell proliferation: Activation of mitogen-activated protein kinase and phosphatidylinositol 3-kinase. Am J Physiol Cell Physiol. 296:C980–C991. 2009. View Article : Google Scholar : PubMed/NCBI
23	Cohen K, Ellis M, Khoury S, Davis PJ, Hercbergs A and Ashur-Fabian O: Thyroid hormone is a MAPK-dependent growth factor for human myeloma cells acting via αvβ3 integrin. Mol Cancer Res. 9:1385–1394. 2011. View Article : Google Scholar : PubMed/NCBI
24	Luidens MK, Mousa SA, Davis FB, Lin HY and Davis PJ: Thyroid hormone and angiogenesis. Vascul Pharmacol. 52:142–145. 2010. View Article : Google Scholar : PubMed/NCBI
25	Sukocheva OA and Carpenter DO: Anti-apoptotic effects of 3,5,3′-tri-iodothyronine in mouse hepatocytes. J Endocrinol. 191:447–458. 2006. View Article : Google Scholar : PubMed/NCBI
26	Hausenloy DJ and Yellon DM: New directions for protecting the heart against ischaemia-reperfusion injury: Targeting the reperfusion injury salvage kinase (RISK)-pathway. Cardiovasc Res. 61:448–460. 2004. View Article : Google Scholar : PubMed/NCBI