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Article

1α,25‑Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow‑derived macrophages

  • Authors:
    • Dong Wang
    • Jian‑Hong Gu
    • Yang Chen
    • Hong‑Yan Zhao
    • Wei Liu
    • Rui‑Long Song
    • Jian‑Chun Bian
    • Xue‑Zhong Liu
    • Yan Yuan
    • Zong‑Ping Liu
  • View Affiliations / Copyright

    Affiliations: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China
  • Pages: 1039-1044
    |
    Published online on: July 8, 2015
       https://doi.org/10.3892/etm.2015.2632
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Abstract

The steroid hormone 1α,25‑dihydroxyvitamin D3 [1α,25‑(OH)2D3] plays an important role in maintaining a balance in calcium and bone metabolism. To study the effects of 1α,25‑(OH)2D3 on osteoclast (OC) formation and bone resorption, OC differentiation was induced in bone marrow‑derived mononuclear cells from Wistar rats with the addition of macrophage colony stimulating factor and receptor activator for nuclear factor‑κB ligand in vitro. Cells were then treated with 1α,25‑(OH)2D3 at 10‑9, 10‑8 or 10‑7 mol/l. OCs were identified using tartrate‑resistant acid phosphatase staining and activity was monitored in the absorption lacunae by scanning electron microscopy. Expression levels of functional proteins associated with bone absorption, namely carbonic anhydrase II, cathepsin K and matrix metalloproteinase‑9 were evaluated by western blot analysis. The results showed that 1α,25‑(OH)2D3 inhibited the formation and activation of OCs in a dose‑dependent manner and downregulated the expression levels of bone absorption‑associated proteins.
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Copy and paste a formatted citation
Spandidos Publications style
Wang D, Gu JH, Chen Y, Zhao HY, Liu W, Song RL, Bian JC, Liu XZ, Yuan Y, Liu ZP, Liu ZP, et al: 1α,25‑Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow‑derived macrophages. Exp Ther Med 10: 1039-1044, 2015.
APA
Wang, D., Gu, J., Chen, Y., Zhao, H., Liu, W., Song, R. ... Liu, Z. (2015). 1α,25‑Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow‑derived macrophages. Experimental and Therapeutic Medicine, 10, 1039-1044. https://doi.org/10.3892/etm.2015.2632
MLA
Wang, D., Gu, J., Chen, Y., Zhao, H., Liu, W., Song, R., Bian, J., Liu, X., Yuan, Y., Liu, Z."1α,25‑Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow‑derived macrophages". Experimental and Therapeutic Medicine 10.3 (2015): 1039-1044.
Chicago
Wang, D., Gu, J., Chen, Y., Zhao, H., Liu, W., Song, R., Bian, J., Liu, X., Yuan, Y., Liu, Z."1α,25‑Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow‑derived macrophages". Experimental and Therapeutic Medicine 10, no. 3 (2015): 1039-1044. https://doi.org/10.3892/etm.2015.2632
Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Gu JH, Chen Y, Zhao HY, Liu W, Song RL, Bian JC, Liu XZ, Yuan Y, Liu ZP, Liu ZP, et al: 1α,25‑Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow‑derived macrophages. Exp Ther Med 10: 1039-1044, 2015.
APA
Wang, D., Gu, J., Chen, Y., Zhao, H., Liu, W., Song, R. ... Liu, Z. (2015). 1α,25‑Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow‑derived macrophages. Experimental and Therapeutic Medicine, 10, 1039-1044. https://doi.org/10.3892/etm.2015.2632
MLA
Wang, D., Gu, J., Chen, Y., Zhao, H., Liu, W., Song, R., Bian, J., Liu, X., Yuan, Y., Liu, Z."1α,25‑Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow‑derived macrophages". Experimental and Therapeutic Medicine 10.3 (2015): 1039-1044.
Chicago
Wang, D., Gu, J., Chen, Y., Zhao, H., Liu, W., Song, R., Bian, J., Liu, X., Yuan, Y., Liu, Z."1α,25‑Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow‑derived macrophages". Experimental and Therapeutic Medicine 10, no. 3 (2015): 1039-1044. https://doi.org/10.3892/etm.2015.2632
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