microRNA‑195 inhibits cell proliferation in bladder cancer via inhibition of cell division control protein 42 homolog/signal transducer and activator of transcription‑3 signaling Retraction in /10.3892/etm.2023.12366

Zhao,Cheng; Qi,Lin; Chen,Minfeng; Liu,Longfei; Yan,Weiqian; Tong,Shiyu; Zu,Xiongbing

doi:10.3892/etm.2015.2633

September-2015 Volume 10 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

September-2015 Volume 10 Issue 3

Full Size Image

Article

microRNA‑195 inhibits cell proliferation in bladder cancer via inhibition of cell division control protein 42 homolog/signal transducer and activator of transcription‑3 signaling

Retraction in: /10.3892/etm.2023.12366

Authors:
- Cheng Zhao
- Lin Qi
- Minfeng Chen
- Longfei Liu
- Weiqian Yan
- Shiyu Tong
- Xiongbing Zu
View Affiliations / Copyright

Affiliations: Department of Urology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China, Department of Neurology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China
Pages: 1103-1108
|
Published online on: July 9, 2015

https://doi.org/10.3892/etm.2015.2633
Expand metrics +

Abstract

microRNA (miR)‑195 acts as a suppressor in multiple types of malignant tumors, including bladder cancer; however, the detailed function of miR‑195 in bladder cancer remains largely unknown. The aim of the present study was to investigate the role of miR‑195 in the regulation of bladder cancer cell proliferation and to determine whether cell division control protein 42 homolog (Cdc42)/signal transducer and activator of transcription‑3 (STAT3) signaling acts as a downstream effector of miR‑195 in bladder cancer cells. Reverse transcription‑quantitative polymerase chain reaction was used to determine the expression levels of miR‑195 in bladder cancer tissues and normal adjacent tissue. The results revealed that the expression of miR‑195 was significantly downregulated in bladder cancer tissues compared with that in the normal adjacent tissues. A luciferase reporter assay was then conducted, which identified Cdc42 as a direct target of miR‑195, and the expression of Cdc42 was significantly upregulated in bladder cancer tissues, as determined by western blotting. Furthermore, miR‑195 negatively regulated the protein expression of Cdc42 in bladder cancer cells. An MTT assay was also conducted to determine the rate of cell proliferation. Upregulation of miR‑195 or the inhibition of Cdc42 could inhibit bladder cancer cell proliferation, possibly through activation of STAT3 signaling. In addition, restoration of Cdc42 could reverse the inhibitory effect of miR‑195 upregulation on bladder cancer cell proliferation. In conclusion, the results of the present study suggest that miR‑195 plays an inhibitory role in the regulation of bladder cancer cell proliferation by directly targeting Cdc42/STAT3 signaling.

View Figures

View References

1	Skeldon SC and Larry Goldenberg S: Bladder cancer: A portal into men's health. Urol Oncol. 33:40–44. 2014. View Article : Google Scholar : PubMed/NCBI
2	Ghafouri-Fard S, Nekoohesh L and Motevaseli E: Bladder cancer biomarkers: review and update. Asian Pac J Cancer Prev. 15:2395–2403. 2014. View Article : Google Scholar : PubMed/NCBI
3	Huang X, Liang M, Dittmar R and Wang L: Extracellular microRNAs in urologic malignancies: chances and challenges. Int J Mol Sci. 14:14785–14799. 2013. View Article : Google Scholar : PubMed/NCBI
4	Ichimi T, Enokida H, Okuno Y, et al: Identification of novel microRNA targets based on microRNA signatures in bladder cancer. Int J Cancer. 125:345–352. 2009. View Article : Google Scholar : PubMed/NCBI
5	Yoshino H, Seki N, Itesako T, Chiyomaru T, Nakagawa M and Enokida H: Aberrant expression of microRNAs in bladder cancer. Nat Rev Urol. 10:396–404. 2013. View Article : Google Scholar : PubMed/NCBI
6	Chabre O, Libé R, Assie G, et al: Serum miR-483-5p and miR-195 are predictive of recurrence risk in adrenocortical cancer patients. Endocr Relat Cancer. 20:579–594. 2013.PubMed/NCBI
7	Yongchun Z, Linwei T, Xicai W, et al: MicroRNA-195 inhibits non-small cell lung cancer cell proliferation, migration and invasion by targeting MYB. Cancer Lett. 347:65–74. 2014. View Article : Google Scholar : PubMed/NCBI
8	Tahiri A, Leivonen SK, Lüders T, et al: Deregulation of cancer-related miRNAs is a common event in both benign and malignant human breast tumors. Carcinogenesis. 35:76–85. 2014. View Article : Google Scholar : PubMed/NCBI
9	Yang G, Wu D, Zhu J, et al: Upregulation of miR-195 increases the sensitivity of breast cancer cells to Adriamycin treatment through inhibition of Raf-1. Oncol Rep. 30:877–889. 2013.PubMed/NCBI
10	Fei X, Qi M, Wu B, Song Y, Wang Y and Li T: MicroRNA-195-5p suppresses glucose uptake and proliferation of human bladder cancer T24 cells by regulating GLUT3 expression. FEBS Lett. 586:392–397. 2012. View Article : Google Scholar : PubMed/NCBI
11	Lin Y, Wu J, Chen H, et al: Cyclin-dependent kinase 4 is a novel target in micoRNA-195-mediated cell cycle arrest in bladder cancer cells. FEBS Lett. 586:442–447. 2012. View Article : Google Scholar : PubMed/NCBI
12	Dogini DB, Pascoal VD, Avansini SH, Vieira AS, Pereira TC and Lopes-Cendes I: The new world of RNAs. Genet Mol Biol. 37 (1 Suppl):285–293. 2014. View Article : Google Scholar : PubMed/NCBI
13	Stengel K and Zheng Y: Cdc42 in oncogenic transformation, invasion, and tumorigenesis. Cell Signal. 23:1415–1423. 2011. View Article : Google Scholar : PubMed/NCBI
14	Wu F, Chen Y, Li Y, Ju J, Wang Z and Yan D: RNA-interference-mediated Cdc42 silencing down-regulates phosphorylation of STAT3 and suppresses growth in human bladder-cancer cells. Biotechnol Appl Biochem. 49:121–128. 2008. View Article : Google Scholar : PubMed/NCBI
15	Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI
16	Baer C, Claus R and Plass C: Genome-wide epigenetic regulation of miRNAs in cancer. Cancer Res. 73:473–477. 2013. View Article : Google Scholar : PubMed/NCBI
17	Han Y, Chen J, Zhao X, et al: MicroRNA expression signatures of bladder cancer revealed by deep sequencing. PLoS One. 6:e182862011. View Article : Google Scholar : PubMed/NCBI
18	Itesako T, Seki N, Yoshino H, et al: The microRNA expression signature of bladder cancer by deep sequencing: The functional significance of the miR-195/497 cluster. PLoS One. 9:e843112014. View Article : Google Scholar : PubMed/NCBI
19	Ni S, Hu J, Duan Y, Shi S, Li R, Wu H, Qu Y and Li Y: Down expression of LRP1B promotes cell migration via RhoA/Cdc42 pathway and actin cytoskeleton remodeling in renal cell cancer. Cancer Sci. 104:817–825. 2013. View Article : Google Scholar : PubMed/NCBI
20	Ma L, Deng X, Wu M, Zhang G and Huang J: Down-regulation of miRNA-204 by LMP-1 enhances CDC42 activity and facilitates invasion of EBV-associated nasopharyngeal carcinoma cells. FEBS Lett. 588:1562–1570. 2014. View Article : Google Scholar : PubMed/NCBI
21	Zins K, Lucas T, Reichl P, Abraham D and Aharinejad S: A Rac1/Cdc42 GTPase-specific small molecule inhibitor suppresses growth of primary human prostate cancer xenografts and prolongs survival in mice. PLoS One. 8:e749242013. View Article : Google Scholar : PubMed/NCBI
22	Volanis D, Zaravinos A, Kadiyska T, Delakas D, Zoumpourlis V and Spandidos DA: Expression profile of Rho kinases in urinary bladder cancer. J BUON. 16:511–521. 2011.PubMed/NCBI
23	Raptis L, Arulanandam R, Geletu M and Turkson J: The R(h)oads to Stat3: Stat3 activation by the Rho GTPases. Exp Cell Res. 317:1787–1795. 2011. View Article : Google Scholar : PubMed/NCBI
24	Arulanandam R, Vultur A, Cao J, et al: Cadherin-cadherin engagement promotes cell survival via Rac1/Cdc42 and signal transducer and activator of transcription-3. Mol Cancer Res. 7:1310–1327. 2009. View Article : Google Scholar : PubMed/NCBI
25	Debidda M, Wang L, Zang H, Poli V and Zheng Y: A role of STAT3 in Rho GTPase-regulated cell migration and proliferation. J Biol Chem. 280:17275–17285. 2005. View Article : Google Scholar : PubMed/NCBI