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Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells

  • Authors:
    • Xiaoyan Dong
    • Ning Ma
    • Mengmeng Liu
    • Ziling Liu
  • View Affiliations / Copyright

    Affiliations: Department of Hematological Neoplasms, The First Affiliated Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1271-1276
    |
    Published online on: July 23, 2015
       https://doi.org/10.3892/etm.2015.2651
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Abstract

The aim of the present study was to explore the preparation of arsenic trioxide (As2O3) nanoparticles and examine the antitumor effects of these nanoparticles on NB4 cells. As2O3 nanoparticles were prepared using the sol‑gel method and characterized using transmission electron microscopy and energy dispersive spectroscopy. The results indicated that the As2O3 nanoparticles prepared in the present study were round or elliptical, well dispersed and had an ~40‑nm or <10‑nm diameter. The antitumor effects of As2O3 nanoparticles at various concentrations were analyzed by flow cytometry and the MTT assay, and were compared with those of traditional As2O3 solution. At the same concentration and incubation time (48 h), the survival rate of cells treated with As2O3 nanoparticles was significantly lower than that of cells treated with the As2O3 solution. The growth inhibition rate under both treatments was time‑ and dose‑dependent. In addition, at the same concentration and incubation time, the apoptosis rate of the cells treated with As2O3 nanoparticles was significantly higher than that of the cells treated with the As2O3 solution. Furthermore, As2O3 nanoparticles resulted in a greater reduction in the expression of the anti‑apoptotic protein B‑cell lymphoma 2 compared with the As2O3 solution. In conclusion, As2O3 nanoparticles, prepared using the sol‑gel method, were found to produce a stronger cytotoxic effect on tumor cells than that produced by the As2O3 solution, possibly by inhibiting Bcl-2 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Dong X, Ma N, Liu M and Liu Z: Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells. Exp Ther Med 10: 1271-1276, 2015.
APA
Dong, X., Ma, N., Liu, M., & Liu, Z. (2015). Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells. Experimental and Therapeutic Medicine, 10, 1271-1276. https://doi.org/10.3892/etm.2015.2651
MLA
Dong, X., Ma, N., Liu, M., Liu, Z."Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells". Experimental and Therapeutic Medicine 10.4 (2015): 1271-1276.
Chicago
Dong, X., Ma, N., Liu, M., Liu, Z."Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells". Experimental and Therapeutic Medicine 10, no. 4 (2015): 1271-1276. https://doi.org/10.3892/etm.2015.2651
Copy and paste a formatted citation
x
Spandidos Publications style
Dong X, Ma N, Liu M and Liu Z: Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells. Exp Ther Med 10: 1271-1276, 2015.
APA
Dong, X., Ma, N., Liu, M., & Liu, Z. (2015). Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells. Experimental and Therapeutic Medicine, 10, 1271-1276. https://doi.org/10.3892/etm.2015.2651
MLA
Dong, X., Ma, N., Liu, M., Liu, Z."Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells". Experimental and Therapeutic Medicine 10.4 (2015): 1271-1276.
Chicago
Dong, X., Ma, N., Liu, M., Liu, Z."Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells". Experimental and Therapeutic Medicine 10, no. 4 (2015): 1271-1276. https://doi.org/10.3892/etm.2015.2651
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