Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October-2015 Volume 10 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2015 Volume 10 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Novel cardioprotective strategy combining three different preconditioning methods to prevent ischemia/reperfusion injury in aged hearts in an improved rabbit model

  • Authors:
    • Jian‑Xi Ye
    • Dao‑Zhong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Surgery, Union Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China
    Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1339-1347
    |
    Published online on: August 13, 2015
       https://doi.org/10.3892/etm.2015.2680
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The use of ischemic preconditioning (IPC) to protect the myocardium is usually not effective in elderly patients. The aim of the present study was to design new methods to achieve enhanced myocardial protection, based on the differential role of endogenous adenosine (ADO) and ADO receptors (ARs) in the effects of IPC on young and old animals. An improved New Zealand white rabbit model of ischemia/reperfusion was established based on the Langendorff model. Adult or elderly rabbit hearts, with or without exposure to IPC, were used in order to assess the roles of ADO and ARs in the different effects of IPC. Different protective methods were designed based on a combination of endogenous and exogenous interventions. Cardiac function, as well as biochemical, histopathological and apoptotic indices, were measured in the different intervention groups. The improved Langendorff model was stable, reliable and suitable for the undertaking of the experiments. The ADO levels in the aged rabbit hearts pre‑ and post‑IPC were lower than those in the adult hearts, indicating that ADO levels may be an endogenous factor influencing IPC. A new protection strategy combining ADO‑enhanced IPC, A1AR agonist 2‑chloro‑N(6)‑cyclopentyladenosine preconditioning and cold crystalloid cardioplegia had a significant protective effect in aged hearts. The results of the present study suggested that endogenous ADO enhancement, A1AR agonist preconditioning and exogenous treatment yield an additive effect in aged rabbit hearts. The simultaneous application of these three types of intervention provided the most effective myocardial protection in the improved aged rabbit heart model.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Go AS, Mozaffarian D, Roger VL, Benjamin EJ, Berry JD, Borden WB, Bravata DM, Dai S, Ford ES, Fox CS, et al: American Heart Association Statistics Committee and Stroke Statistics Subcommittee: Heart disease and stroke statistics − 2013 update: A report from the American Heart Association. Circulation. 127:e6–e245. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Liu M, Zhang P, Chen M, et al: Aging might increase myocardial ischemia/reperfusion-induced apoptosis in humans and rats. Age (Dordr). 34:621–632. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Marczak J, Nowicki R, Kulbacka J and Saczko J: Is remote ischaemic preconditioning of benefit to patients undergoing cardiac surgery? Interact Cardiovasc Thorac Surg. 14:634–639. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Han S, Huang W, Liu Y, Pan S, Feng Z and Li S: Does leukocyte-depleted blood cardioplegia reduce myocardial reperfusion injury in cardiac surgery? A systematic review and meta-analysis. Perfusion. 28:474–483. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Murry CE, Jennings RB and Reimer KA: Preconditioning with ischemia: A delay of lethal cell injury in ischemic myocardium. Circulation. 74:1124–1136. 1986. View Article : Google Scholar : PubMed/NCBI

6 

Yellon DM and Downey JM: Preconditioning the myocardium: From cellular physiology to clinical cardiology. Physiol Rev. 83:1113–1151. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Qiu Y, Tang XL, Park SW, Sun JZ, Kalya A and Bolli R: The early and late phases of ischemic preconditioning: A comparative analysis of their effects on infarct size, myocardial stunning and arrhythmias in conscious pigs undergoing a 40-minute coronary occlusion. Circ Res. 80:730–742. 1997. View Article : Google Scholar : PubMed/NCBI

8 

Zhang X, Xiao Z, Yao J, Zhao G, Fa X and Niu J: Participation of protein kinase C in the activation of Nrf2 signaling by ischemic preconditioning in the isolated rabbit heart. Mol Cell Biochem. 372:169–179. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Guo Y, Wu WJ, Qiu Y, Tang XL, Yang Z and Bolli R: Demonstration of an early and a late phase of ischemic preconditioning in mice. Am J Physiol. 275:H1375–H1387. 1998.PubMed/NCBI

10 

Bolli R: Cardioprotective function of inducible nitric oxide synthase and role of nitric oxide in myocardial ischemia and preconditioning: An overview of a decade of research. J Mol Cell Cardiol. 33:1897–1918. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Abete P, Cacciatore F, Testa G, DellaMorte D, Galizia G, Ferrara N and Rengo F: Clinical application of ischemic preconditioning in the elderly. Dose Response. 8:34–40. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Dawson R Jr and Meldrum MJ: Norepinephrine content in cardiovascular tissues from the aged Fischer 344 rat. Gerontology. 38:185–191. 1992. View Article : Google Scholar : PubMed/NCBI

13 

Tani M, Honma Y, Hasegawa H and Tamaki K: Direct activation of mitochondrial K (ATP) channels mimics preconditioning but protein kinase C activation is less effective in middle-aged rat hearts. Cardiovasc Res. 49:56–68. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Sadigh B, Quintana M, Sylvén C, Berglund M and Brodin LA: The ischemic preconditioning effect of adenosine in patients with ischemic heart disease. Cardiovasc Ultrasound. 7:522009. View Article : Google Scholar : PubMed/NCBI

15 

McCully JD, Toyoda Y, Uematsu M, Stewart RD and Levitsky S: Adenosine-enhanced ischemic preconditioning: Adenosine receptor involvement during ischemia and reperfusion. Am J Physiol Heart Circ Physiol. 280:H591–H602. 2001.PubMed/NCBI

16 

Schulman D, Latchman DS and Yellon DM: Effect of aging on the ability of preconditioning to protect rat hearts from ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 281:H1630–H1636. 2001.PubMed/NCBI

17 

Boengler K, Schulz R and Heusch G: Loss of cardioprotection with ageing. Cardiovasc Res. 83:247–261. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Langendorff O: Untersuchungen am uberlebenden Saugethierherzen. Pflugers Archives fur die Gesamte Physiologie des Menschen and der Tiere. 61:291–332. 1895.(In German). View Article : Google Scholar

19 

Suzuki Y, Yeung AC and Ikeno F: The pre-clinical animal model in the translational research of interventional cardiology. JACC Cardiovasc Interv. 2:373–383. 2009. View Article : Google Scholar : PubMed/NCBI

20 

SkrzypiecSpring M, Grotthus B, Szelag A and Schulz R: Isolated heart perfusion according to Langendorff-still viable in the new millennium. J Pharmacol Toxicol Methods. 55:113–126. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Chorro FJ, SuchBelenguer L and López-Merino V: Animal models of cardiovascular disease. Rev Esp Cardiol. 62:69–84. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Bell RM, Mocanu MM and Yellon DM: Retrograde heart perfusion: The Langendorff technique of isolated heart perfusion. J Mol Cell Cardiol. 50:940–950. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Hancu G, Simon B, Kelemen H, Rusu A, Mircia E and Gyeresi A: Thin layer chromatographic analysis of Beta-lactam antibiotics. Adv Pharm Bull. 3:367–371. 2013.PubMed/NCBI

24 

Kaneko M, Shintani Y, Narita T, et al: Extracellular high mobility group box 1 plays a role in the effect of bone marrow mononuclear cell transplantation for heart failure. PLoS One. 8:e769082013. View Article : Google Scholar : PubMed/NCBI

25 

Bertazzo S, Gentleman E, Cloyd KL, Chester AH, Yacoub MH and Stevens MM: Nano-analytical electron microscopy reveals fundamental insights into human cardiovascular tissue calcification. Nat Mater. 12:576–583. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Liao R, Podesser BK and Lim CC: The continuing evolution of the Langendorff and ejecting murine heart: New advances in cardiac phenotyping. Am J Physiol Heart Circ Physiol. 303:H156–H167. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Fredholm BB, Abbracchio MP, Burnstock G, et al: Nomenclature and classification of purinoceptors. Pharmacol Rev. 46:143–156. 1994.PubMed/NCBI

28 

VintenJohansen J, Thourani VH, Ronson RS, et al: Broad-spectrum cardioprotection with adenosine. Ann Thorac Surg. 68:1942–1948. 1999. View Article : Google Scholar : PubMed/NCBI

29 

Lasley RD, Keith BJ, Kristo G, Yoshimura Y and Mentzer RM Jr: Delayed adenosine A1 receptor preconditioning in rat myocardium is MAPK dependent but iNOS independent. Am J Physiol Heart Circ Physiol. 289:H785–791. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Tomai F, Crea F, Chiariello L and Gioffrè PA: Ischemic preconditioning in humans: Models, mediators and clinical relevance. Circulation. 100:559–563. 1999. View Article : Google Scholar : PubMed/NCBI

31 

Yun N, Kim SH and Lee SM: Differential consequences of protein kinase C activation during early and late hepatic ischemic preconditioning. J Physiol Sci. 62:199–209. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Naderi R, Imani A, Faghihi M and Moghimian M: Phenylephrine induces early and late cardioprotection through mitochondrial permeability transition pore in the isolated rat heart. J Surg Res. 164:e37–e42. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ye JX and Chen DZ: Novel cardioprotective strategy combining three different preconditioning methods to prevent ischemia/reperfusion injury in aged hearts in an improved rabbit model. Exp Ther Med 10: 1339-1347, 2015.
APA
Ye, J., & Chen, D. (2015). Novel cardioprotective strategy combining three different preconditioning methods to prevent ischemia/reperfusion injury in aged hearts in an improved rabbit model. Experimental and Therapeutic Medicine, 10, 1339-1347. https://doi.org/10.3892/etm.2015.2680
MLA
Ye, J., Chen, D."Novel cardioprotective strategy combining three different preconditioning methods to prevent ischemia/reperfusion injury in aged hearts in an improved rabbit model". Experimental and Therapeutic Medicine 10.4 (2015): 1339-1347.
Chicago
Ye, J., Chen, D."Novel cardioprotective strategy combining three different preconditioning methods to prevent ischemia/reperfusion injury in aged hearts in an improved rabbit model". Experimental and Therapeutic Medicine 10, no. 4 (2015): 1339-1347. https://doi.org/10.3892/etm.2015.2680
Copy and paste a formatted citation
x
Spandidos Publications style
Ye JX and Chen DZ: Novel cardioprotective strategy combining three different preconditioning methods to prevent ischemia/reperfusion injury in aged hearts in an improved rabbit model. Exp Ther Med 10: 1339-1347, 2015.
APA
Ye, J., & Chen, D. (2015). Novel cardioprotective strategy combining three different preconditioning methods to prevent ischemia/reperfusion injury in aged hearts in an improved rabbit model. Experimental and Therapeutic Medicine, 10, 1339-1347. https://doi.org/10.3892/etm.2015.2680
MLA
Ye, J., Chen, D."Novel cardioprotective strategy combining three different preconditioning methods to prevent ischemia/reperfusion injury in aged hearts in an improved rabbit model". Experimental and Therapeutic Medicine 10.4 (2015): 1339-1347.
Chicago
Ye, J., Chen, D."Novel cardioprotective strategy combining three different preconditioning methods to prevent ischemia/reperfusion injury in aged hearts in an improved rabbit model". Experimental and Therapeutic Medicine 10, no. 4 (2015): 1339-1347. https://doi.org/10.3892/etm.2015.2680
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team