search button

Effect of TNF-α inhibitors on transcriptional levels of pro-inflammatory interleukin-33 and Toll-like receptors-2 and -9 in psoriatic plaques

  • Authors:
    • Dimitra P. Vageli
    • Aikaterini Exarchou
    • Efterpi Zafiriou
    • Panagiotis G. Doukas
    • Sotirios Doukas
    • Angeliki Roussaki‑Schulze

  • View Affiliations

  • Published online on: August 18, 2015     https://doi.org/10.3892/etm.2015.2688
  • Pages: 1573-1577
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Tumor necrosis factor (TNF)-α inhibitors are considered to be effective in the treatment of psoriatic plaques, although the precise therapeutic pathway is not clear. Pro-inflammatory molecules, such as Toll‑like receptor (TLR)-2 and -9 and interleukin (IL)-33, a member of the IL-1 receptor/TLR superfamily, have been found to be expressed in psoriatic plaques. The aim of the present study was to investigate whether TNF‑α inhibitor treatment has an effect on the expression of IL‑33 and TLR‑2 and ‑9 in psoriatic plaques. Seventeen patients with psoriatic plaques were treated with a TNF‑α inhibitor (etanercept or infliximab) for 12 weeks in an open‑label study, and the transcriptional levels of IL‑33 and TLR‑2 and ‑9 were determined by reverse transcription‑quantitative polymerase chain reaction in paired biopsies of psoriatic plaques obtained at baseline (B) and following the 12 weeks of treatment (P). The psoriasis area severity index (PASI) score was also determined. At B, elevated IL‑33 and TLR‑2 mRNA levels were observed in all cases, while TLR‑9 showed elevated mRNA levels in 76% of cases. At P, reductions in the mRNA levels of IL‑33, TLR‑2 and TLR‑9 were observed, with TLR‑2 and ‑9 levels exhibiting significant reductions (P<0.0001, Wilcoxon signed‑rank test). PASI scores were significantly reduced by the treatment (P<0.0001, Wilcoxon signed‑rank test) and the changes in PASI scores exhibited a significant positive Pearson's correlation with the P/B mRNA expression ratios of TLR-2 or -9 in males (P<0.05), particularly in the etanercept group (P<0.0001). The findings support the efficacy of anti-TNF-α treatment on the innate immune response in psoriatic skin, with a focus on TLR-2 and -9 inhibition, suggesting their role in the pathogenic mechanism of plaque psoriasis, which may be associated with gender.
View Figures
View References

Related Articles

Journal Cover

October-2015
Volume 10 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 2.7
  • Ranked Medicine Research and Experimental
    (total number of cites)
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Vageli DP, Exarchou A, Zafiriou E, Doukas PG, Doukas S and Roussaki‑Schulze A: Effect of TNF-α inhibitors on transcriptional levels of pro-inflammatory interleukin-33 and Toll-like receptors-2 and -9 in psoriatic plaques. Exp Ther Med 10: 1573-1577, 2015
APA
Vageli, D.P., Exarchou, A., Zafiriou, E., Doukas, P.G., Doukas, S., & Roussaki‑Schulze, A. (2015). Effect of TNF-α inhibitors on transcriptional levels of pro-inflammatory interleukin-33 and Toll-like receptors-2 and -9 in psoriatic plaques. Experimental and Therapeutic Medicine, 10, 1573-1577. https://doi.org/10.3892/etm.2015.2688
MLA
Vageli, D. P., Exarchou, A., Zafiriou, E., Doukas, P. G., Doukas, S., Roussaki‑Schulze, A."Effect of TNF-α inhibitors on transcriptional levels of pro-inflammatory interleukin-33 and Toll-like receptors-2 and -9 in psoriatic plaques". Experimental and Therapeutic Medicine 10.4 (2015): 1573-1577.
Chicago
Vageli, D. P., Exarchou, A., Zafiriou, E., Doukas, P. G., Doukas, S., Roussaki‑Schulze, A."Effect of TNF-α inhibitors on transcriptional levels of pro-inflammatory interleukin-33 and Toll-like receptors-2 and -9 in psoriatic plaques". Experimental and Therapeutic Medicine 10, no. 4 (2015): 1573-1577. https://doi.org/10.3892/etm.2015.2688