Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives

Rogers‑Broadway,Karly‑Rai; Karteris,Emmanouil

doi:10.3892/etm.2015.2712

October-2015 Volume 10 Issue 4

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October-2015 Volume 10 Issue 4

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Article Open Access

Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives

Authors:
- Karly‑Rai Rogers‑Broadway
- Emmanouil Karteris
View Affiliations / Copyright

Affiliations: Department of Biosciences, Brunel University, Uxbridge UB8 3PH, UK

Copyright: © Rogers‑Broadway et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 1261-1264
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Published online on: August 25, 2015

https://doi.org/10.3892/etm.2015.2712
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Abstract

Quantitative polymerase chain reaction (qPCR) is a method of amplifying and detecting small samples of genetic material in real time and is in routine use across many laboratories. Speed and thermal uniformity, two important factors in a qPCR test, are in direct conflict with one another in conventional peltier‑driven thermal cyclers. To overcome this, companies are developing novel thermal systems for qPCR testing. More recently, qPCR technology has developed to enable its use in point‑of‑care testing (POCT), where the test is administered and results are obtained in a single visit to a health provider, particularly in developing countries. For a system to be suitable for POCT it must be rapid and reliable. In the present study, the speed and thermal uniformity of four qPCR thermal cyclers currently available were compared, two of which use the conventional peltier/block heating method and two of which use novel heating and cooling methods. The time required to complete 40 cycles varied between 12 and 58 min, and the Ct values were comparable, ranging between 13.6 and 16.8. Therefore, the novel technologies investigated in the present study for qPCR instrumentation performed equally well compared with conventional qPCR instruments, in terms of amplification efficiency and thermal uniformity.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives

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