Phylogenetic reconstruction and polymorphism analysis of BK virus VP2 gene isolated from renal transplant recipients in China

Wang,Zhang‑Yang; Hong,Wei‑Long; Zhu,Zhe‑Hui; Chen,Yun‑Hao; Ye,Wen‑Le; Chu,Guang‑Yu; Li,Jia‑Lin; Chen,Bi‑Cheng; Xia,Peng

doi:10.3892/etm.2015.2723

Phylogenetic reconstruction and polymorphism analysis of BK virus VP2 gene isolated from renal transplant recipients in China

Authors:
- Zhang‑Yang Wang
- Wei‑Long Hong
- Zhe‑Hui Zhu
- Yun‑Hao Chen
- Wen‑Le Ye
- Guang‑Yu Chu
- Jia‑Lin Li
- Bi‑Cheng Chen
- Peng Xia
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Affiliations: Zhejiang Provincial Top Key Discipline in Surgery, Wenzhou Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Transplantation Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
Published online on: September 1, 2015 https://doi.org/10.3892/etm.2015.2723
Pages: 1759-1767
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

BK polyomavirus (BKV) is important pathogen for kidney transplant recipients, as it is frequently re‑activated, leading to nephropathy. The aim of this study was to investigate the phylogenetic reconstruction and polymorphism of the VP2 gene in BKV isolated from Chinese kidney transplant recipients. Phylogenetic analysis was carried out in the VP2 region from 135 BKV‑positive samples and 28 reference strains retrieved from GenBank. The unweighted pair‑group method with arithmetic mean (UPGMA) grouped all strains into subtypes, but failed to subdivide strains into subgroups. Among the plasma and urine samples, all plasma (23/23) and 82 urine samples (82/95) were identified to contain subtype I; the other 10 urine samples contained subtype IV. A 86‑bp fragment was identified as a highly conserved sequence. Following alignment with 36 published BKV sequences from China, 92 sites of polymorphism were identified, including 11 single nucleotide polymorphisms (SNPs) prevalent in Chinese individuals and 30 SNPs that were specific to the two predominant subtypes I and IV. The limitations of the VP2 gene segment in subgrouping were confirmed by phylogenetic analysis. The conserved sequence and polymorphism identified in this study may be helpful in the detection and genotyping of BKV.

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