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Article

Protective effects of Tougu Xiaotong capsule on tumor necrosis factor‑α‑injured UMR‑106 cells

  • Authors:
    • Naishun Liao
    • Yunmei Huang
    • Jinxia Ye
    • Wenlie Chen
    • Zuan Fang Li
    • Ruhui Lin
    • Xihai Li
    • Liangpu Zheng
    • Xianxiang Liu
  • View Affiliations / Copyright

    Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China
  • Pages: 1908-1914
    |
    Published online on: September 10, 2015
       https://doi.org/10.3892/etm.2015.2739
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Abstract

Tumor necrosis factor‑α (TNF‑α) plays an important role in the abnormal metabolism of osteoblasts (OBs), which leads to subchondral bone (SB) alterations in osteoarthritis. In the present study, Tougu Xiaotong capsule (TXC), a traditional Chinese medicine, was used to treat TNF‑α‑injured OB‑like cells. The cellular viability, mortality and ultramicroscopic morphology were evaluated. Thereafter, the activity of alkaline phosphatase (ALP), secretion of osteocalcin (OCN) and mineralization of nodules were analyzed. The results showed that TXC treatment significantly promoted cell proliferation, reduced cellular mortality and improved cellular ultrastructure, particularly that of the endoplasmic reticulum and nucleus. These data indicate that TXC is able to promote cell growth, as well as prevent inflammation in OB‑like cells. Furthermore, the activity of ALP, secretion of OCN and mineralization of nodules were accelerated, and the calcium content of the TNF‑α‑injured OB‑like cells was promoted by TXC treatment. These results indicate that TXC protected the OB‑like cells from TNF‑α‑induced injuries. This may be a potential mechanism through which TXC regulates SB remodeling in the clinical treatment of osteoarthritis.
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Copy and paste a formatted citation
Spandidos Publications style
Liao N, Huang Y, Ye J, Chen W, Li ZF, Lin R, Li X, Zheng L and Liu X: Protective effects of Tougu Xiaotong capsule on tumor necrosis factor‑α‑injured UMR‑106 cells. Exp Ther Med 10: 1908-1914, 2015.
APA
Liao, N., Huang, Y., Ye, J., Chen, W., Li, Z.F., Lin, R. ... Liu, X. (2015). Protective effects of Tougu Xiaotong capsule on tumor necrosis factor‑α‑injured UMR‑106 cells. Experimental and Therapeutic Medicine, 10, 1908-1914. https://doi.org/10.3892/etm.2015.2739
MLA
Liao, N., Huang, Y., Ye, J., Chen, W., Li, Z. F., Lin, R., Li, X., Zheng, L., Liu, X."Protective effects of Tougu Xiaotong capsule on tumor necrosis factor‑α‑injured UMR‑106 cells". Experimental and Therapeutic Medicine 10.5 (2015): 1908-1914.
Chicago
Liao, N., Huang, Y., Ye, J., Chen, W., Li, Z. F., Lin, R., Li, X., Zheng, L., Liu, X."Protective effects of Tougu Xiaotong capsule on tumor necrosis factor‑α‑injured UMR‑106 cells". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1908-1914. https://doi.org/10.3892/etm.2015.2739
Copy and paste a formatted citation
x
Spandidos Publications style
Liao N, Huang Y, Ye J, Chen W, Li ZF, Lin R, Li X, Zheng L and Liu X: Protective effects of Tougu Xiaotong capsule on tumor necrosis factor‑α‑injured UMR‑106 cells. Exp Ther Med 10: 1908-1914, 2015.
APA
Liao, N., Huang, Y., Ye, J., Chen, W., Li, Z.F., Lin, R. ... Liu, X. (2015). Protective effects of Tougu Xiaotong capsule on tumor necrosis factor‑α‑injured UMR‑106 cells. Experimental and Therapeutic Medicine, 10, 1908-1914. https://doi.org/10.3892/etm.2015.2739
MLA
Liao, N., Huang, Y., Ye, J., Chen, W., Li, Z. F., Lin, R., Li, X., Zheng, L., Liu, X."Protective effects of Tougu Xiaotong capsule on tumor necrosis factor‑α‑injured UMR‑106 cells". Experimental and Therapeutic Medicine 10.5 (2015): 1908-1914.
Chicago
Liao, N., Huang, Y., Ye, J., Chen, W., Li, Z. F., Lin, R., Li, X., Zheng, L., Liu, X."Protective effects of Tougu Xiaotong capsule on tumor necrosis factor‑α‑injured UMR‑106 cells". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1908-1914. https://doi.org/10.3892/etm.2015.2739
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