Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2015 Volume 10 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2015 Volume 10 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Significance of the expression of MRP1 and MRP2 in peripheral blood mononuclear cells of children with intractable epilepsy

  • Authors:
    • Xiaoming Liu
    • Xuan Yue
    • Shengzhi Chen
    • Jiao Chen
    • Rui Li
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Xuzhou Children's Hospital, Xuzhou, Jiangsu 221002, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1784-1788
    |
    Published online on: September 15, 2015
       https://doi.org/10.3892/etm.2015.2746
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Intactable epilepsy (IE) is relatively common in pediatric epilepsy. The resistance mechanism of IE has been previously investigated. Multidrug‑resistant associated protein 1 (MRP1) and MRP2 are associated with drug transport. The aim of the present study was to investigate the expression of MRP1 and MRP2 in peripheral blood mononuclear cells of children with IE. Fifty outpatient or inpatient children were included in the study as the experimental group. Additionally, 50 children with epilepsy controlled by anti‑epileptic drugs (AEDs) and 50 healthy children without epilepsy, who served as the control group, were included in the present study. Expression of MRP1 and MRP2 in the peripheral blood mononuclear cells of children in all the groups was detected using RT-PCR and western blot analysis. The results showed that the relative expression of MRP1 and MRP2 mRNA in the peripheral blood mononuclear cells of children with IE (MRP1, 0.795±0.042; MRP2, 0.804±0.023) was higher than that in epilepsy controlled by AEDs (MRP1, 0.682±0.030; MRP2, 0.675±0.021) and healthy children without epilepsy (MRP1, 0.665±0.031; MRP2, 0.654±0.029) (P<0.01). The mean relative expression of MRP1 and MRP2 protein in the peripheral blood mononuclear cells of children with IE (MRP1, 2.027±0.034; MRP2, 1.902±0.021) was higher than that in children with epilepsy controlled by AEDs (MRP1, 1.131±0.042; MRP2, 1.086±0.027) and healthy children without epilepsy (MRP1, 1.093±0.023; MRP2, 1.045±0.018) (P<0.01). The difference in the MRP1 and MRP2 mRNA and protein expression between the children with epilepsy controlled by AEDs and healthy children without epilepsy was not statistically significant (P>0.05). In conclusion, a higher expression of MRP1 and MRP2 in the peripheral blood mononuclear cells of children with IE may be relevant to the drug-resistant mechanism of IE.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Sugano H and Arai H: Epilepsy surgery for pediatric epilepsy: Optimal timing of surgical intervention. Neurol Med Chir (Tokyo). 55:399–406. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Epilepsy: Benefits and risks of reoperation after failed surgery for intractable epilepsy. Nat Rev Neurol. 11:2472015. View Article : Google Scholar : PubMed/NCBI

3 

Sebe JY and Baraban SC: The promise of an interneuron-based cell therapy for epilepsy. Dev Neurobiol. 71:107–117. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Jose M and Thomas SV: Role of multidrug transporters in neurotherapeutics. Ann Indian Acad Neurol. 12:89–98. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Xu J, Deng Y and Gao B: Blood-CSF barrier related translocator and intractable epilepsy. J Appl Clin Pediatr. 27:1900–1902. 2012.

6 

Luna-Tortós C, Fedrowitz M and Löscher W: Evaluation of transport of common antiepileptic drugs by human multidrug resistance-associated proteins (MRP1, 2 and 5) that are overexpressed in pharmacoresistant epilepsy. Neuropharmacology. 58:1019–1032. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Marquez B and Van Bambeke F: ABC multidrug transporters: target for modulation of drug pharmacokinetics and drug-drug interactions. Curr Drug Targets. 12:600–620. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Schinkel AH and Jonker JW: Mammalian drug efflux transporters of the ATP binding cassette (ABC) family: An overview. Adv Drug Deliv Rev. 55:3–29. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Keppler D: Multidrug resistance proteins (MRPs, ABCCs): Importance for pathophysiology and drug therapy. Handbook Exp Pharmacol. 201:299–323. 2011. View Article : Google Scholar

10 

Kwan P, Arzimanoglou A, Berg AT, Brodie MJ, Allen Hauser W, Mathern G, Moshé SL, Perucca E, Wiebe S and French J: Definition of drug resistant epilepsy: Consensus proposal by the ad hoc Task Force of the ILAE Commission on Therapeutic Strategies. Epilepsia. 51:1069–1077. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Rong H, Jin L, Wei W, Wang X and Xi Z: Alpha-synuclein is a potential biomarker in the serum and CSF of patients with intractable epilepsy. Seizure. 27:6–9. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Grote A, Witt JA, Surges R, von Lehe M, Pieper M, Elger CE, Helmstaedter C, Ormond DR, Schramm J and Delev D: A second chance-reoperation in patients with failed surgery for intractable epilepsy: long-term outcome, neuropsychology and complications. J Neurol Neurosurg Psychiatry. pii:jnnp-2015–310322. 2015.

13 

Fernandez L, Gedela S, Tamber M and Sogawa Y: Vagus nerve stimulation in children less than 3 years with medically intractable epilepsy. Epilepsy Res. 112:37–42. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Yi JH, Cho YJ, Kim WJ, Lee MG and Lee JH: Genetic variations of ABCC2 gene associated with adverse drug reactions to valproic acid in Korean epileptic patients. Genomics Inform. 11:254–262. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Chen YH, Wang CC, Xiao X, Wei L and Xu G: Multidrug resistance-associated protein 1 decreases the concentrations of antiepileptic drugs in cortical extracellular fluid in amygdale kindling rats. Acta Pharmacol Sin. 34:473–479. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Xu Y, Wang L, Zheng X, Liu G, Wang Y, Lai X and Li J: Positive expression of p53, c-erbB2 and MRP proteins is correlated with survival rates of NSCLC patients. Mol Clin Oncol. 1:487–492. 2013.PubMed/NCBI

17 

Kovalev AA, Tsvetaeva DA and Grudinskaja TV: Role of ABC-cassette transporters (MDR1, MRP1, BCRP) in the development of primary and acquired multiple drug resistance in patients with early and metastatic breast cancer. Exp Oncol. 35:287–290. 2013.PubMed/NCBI

18 

Nasilowska-Adamska B, Solarska I, Paluszewska M, Malinowska I, Jedrzejczak WW and Warzocha K: FLT3-ITD and MLL-PTD influence the expression of MDR-1, MRP-1, and BCRP mRNA but not LRP mRNA assessed with RQ-PCR method in adult acute myeloid leukemia. Ann Hematol. 93:577–593. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Calatozzolo C, Pollo B, Botturi A, Dinapoli L, Carosi M, Salmaggi A and Maschio M: Multidrug resistance proteins expression in glioma patients with epilepsy. J Neurooncol. 110:129–135. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Yao D, Liu L, Jin S, Li J and Liu XD: Overexpression of multidrug resistance-associated protein 2 in the brain of pentylenetetrazole-kindled rats. Neuroscience. 227:283–292. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Zhang Y, Dai Y, Rui L, et al: Effect of Oxcarbazepine on multidrug resistance associated protein 1 expression in hippocampus of kainic acid-induced seizure in rats during development period rats. Chin J Appl Clin. 28:1170–1171. 2013.

22 

Zhan L, Ping W and Jing Z: The expression of multi-drug resistance transmembrane transporter in the peripheral blood of patients with multi-drug resistant tuberculosis. Zhonghua Jie He He Hu Xi Za Zhi. 34:520–522. 2011.(In Chinese). PubMed/NCBI

23 

Song Y, Bing L and Zhong L: Expression of multi-drug resistance-associated protein genein acute leukemia. Acta Acad Med Qingdao Univ. 37:41–43. 2001.

24 

Ban JJ, Jung KH, Chu K, Lee ST, Jeon D, Park KI, Moon HJ, Kim H, Kim S, Lee SK, et al: Profiles of multidrug resistance protein-1 in the peripheral blood mononuclear cells of patients with refractory epilepsy. PLoS One. 7:e369852012. View Article : Google Scholar : PubMed/NCBI

25 

Murakami N, Morioka T, Hashiguchi K, Suzuki SO, Shigeto H, Sakata A and Sasaki T: Clinical and histological characteristics of ictal onset zone in cases of intractable epilepsy associated with dysembryoplastic neuroepithelial tumor. Brain Nerve. 67:525–532. 2015.(In Japanese). PubMed/NCBI

26 

Mateo-Carrasco H, Serrano-Castro PJ, Molina-Cuadrado E, Goodwin M, Nguyen TV and Kotecha PN: Role of high-dose levetiracetam as add-on therapy for intractable epilepsy: Case report and brief review of the literature. Int J Clin Pharm. 37:559–562. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Meador KJ, Kapur R, Loring DW, Kanner AM and Morrell MJ: RNS® System Pivotal Trial Investigators: Quality of life and mood in patients with medically intractable epilepsy treated with targeted responsive neurostimulation. Epilepsy Behav. 45:242–247. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Lan T, Teng M, Song J, et al: The expression of multidrug resistance protein-1 in peripheral blood of patients with intractable epilepsy. Zhonghua Jie He He Hu Xi Za Zhi. 39:403–404. 2006.(In Chinese).

29 

van Vliet EA, Redeker S, Aronica E, Edelbroek PM and Gorter JA: Expression of multidrug transporters MRP1, MRP2, and BCRP shortly after status epilepticus, during the latent period, and in chronic epileptic rats. Epilepsia. 46:1569–1580. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Lazarowski A, Lubieniecki F, Camarero S, Pomata H, Bartuluchi M, Sevlever G and Taratuto AL: Multidrug resistance proteins in tuberous sclerosis and refractory epilepsy. Pediatr Neurol. 30:102–106. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Qu J, Zhou BT, Yin JY, Xu XJ, Zhao YC, Lei GH, Tang Q, Zhou HH and Liu ZQ: ABCC2 polymorphisms and haplotype are associated with drug resistance in Chinese epileptic patients. CNS Neurosci Ther. 18:647–651. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu X, Yue X, Chen S, Chen J and Li R: Significance of the expression of MRP1 and MRP2 in peripheral blood mononuclear cells of children with intractable epilepsy. Exp Ther Med 10: 1784-1788, 2015.
APA
Liu, X., Yue, X., Chen, S., Chen, J., & Li, R. (2015). Significance of the expression of MRP1 and MRP2 in peripheral blood mononuclear cells of children with intractable epilepsy. Experimental and Therapeutic Medicine, 10, 1784-1788. https://doi.org/10.3892/etm.2015.2746
MLA
Liu, X., Yue, X., Chen, S., Chen, J., Li, R."Significance of the expression of MRP1 and MRP2 in peripheral blood mononuclear cells of children with intractable epilepsy". Experimental and Therapeutic Medicine 10.5 (2015): 1784-1788.
Chicago
Liu, X., Yue, X., Chen, S., Chen, J., Li, R."Significance of the expression of MRP1 and MRP2 in peripheral blood mononuclear cells of children with intractable epilepsy". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1784-1788. https://doi.org/10.3892/etm.2015.2746
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Yue X, Chen S, Chen J and Li R: Significance of the expression of MRP1 and MRP2 in peripheral blood mononuclear cells of children with intractable epilepsy. Exp Ther Med 10: 1784-1788, 2015.
APA
Liu, X., Yue, X., Chen, S., Chen, J., & Li, R. (2015). Significance of the expression of MRP1 and MRP2 in peripheral blood mononuclear cells of children with intractable epilepsy. Experimental and Therapeutic Medicine, 10, 1784-1788. https://doi.org/10.3892/etm.2015.2746
MLA
Liu, X., Yue, X., Chen, S., Chen, J., Li, R."Significance of the expression of MRP1 and MRP2 in peripheral blood mononuclear cells of children with intractable epilepsy". Experimental and Therapeutic Medicine 10.5 (2015): 1784-1788.
Chicago
Liu, X., Yue, X., Chen, S., Chen, J., Li, R."Significance of the expression of MRP1 and MRP2 in peripheral blood mononuclear cells of children with intractable epilepsy". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1784-1788. https://doi.org/10.3892/etm.2015.2746
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team