Puerarin attenuates the inflammatory response and apoptosis in LPS-stimulated cardiomyocytes

Yuan,Yuan; Zhou,Heng; Wu,Qing‑Qing; Li,Fang‑Fang; Bian,Zhou‑Yan; Deng,Wei; Zhou,Meng‑Qiao; Tang,Qi‑Zhu

doi:10.3892/etm.2015.2910

Puerarin attenuates the inflammatory response and apoptosis in LPS-stimulated cardiomyocytes

Authors:
- Yuan Yuan
- Heng Zhou
- Qing‑Qing Wu
- Fang‑Fang Li
- Zhou‑Yan Bian
- Wei Deng
- Meng‑Qiao Zhou
- Qi‑Zhu Tang
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Affiliations: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
Published online on: December 4, 2015 https://doi.org/10.3892/etm.2015.2910
Pages: 415-420
Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Patients with septic shock suffer from high mortality rates, particularly when complicated by severe myocardial depression which is characterized by hypotension and a reduction in cardiac output. Inflammation is an important factor involved in the early stages of sepsis. The aim of the present study was to investigate the effect of the Chinese herbal compound puerarin (1, 5, 10, 20 and 40 µM) on cardiomyocyte inflammatory response in a sepsis model using H9c2 cardiomyocytes stimulated with 1 µg/ml lipopolysaccharide (LPS). The mRNA expression levels of tumor necrosis factor (TNF)‑α and interleukin (IL)‑β were evaluated using reverse transcription‑quantitative polymerase chain reaction. In addition, the protein expression levels of various factors were determined using western blot analysis. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was used to evaluate the apoptosis rates in the various groups, and immunocytochemical analysis was employed to determine the effect of puerarin on the nuclear translocation of p65 protein. The present study demonstrated that LPS stimulation increased IL‑1β and TNF‑α mRNA expression levels, as compared with the controls (P<0.05). Following treatment with various concentrations of puerarin, the expression levels of IL‑1β and TNF‑α were markedly blunted, particularly in the LPS + 40 µM puerarin group (P<0.05 vs. the LPS group). Furthermore, puerarin administration significantly inhibited LPS‑induced apoptosis in H9c2 cardiomyocytes, as determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining (TUNEL positive cells: LPS + 40 µM puerarin group, 5.5% vs. LPS group, 10.5%; P<0.01). In addition, puerarin significantly decreased LPS‑induced phosphorylated nuclear factor (p‑NF)‑κB p65 and Bax expression levels, and increased the expression levels of Bcl-2, as compared with the LPS group (P<0.05). These data indicated that puerarin may serve as a valuable protective agent against cardiovascular inflammatory diseases.

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