Expression levels of cytokines and chemokines increase in human peripheral blood mononuclear cells stimulated by activation of the Toll-like receptor 5 pathway

Ma,Yu; Zhang,Li; Li,Quansheng

doi:10.3892/etm.2015.2914

February-2016 Volume 11 Issue 2

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February-2016 Volume 11 Issue 2

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Article

Expression levels of cytokines and chemokines increase in human peripheral blood mononuclear cells stimulated by activation of the Toll-like receptor 5 pathway

Authors:
- Yu Ma
- Li Zhang
- Quansheng Li
View Affiliations / Copyright

Affiliations: Department of Thyroid and Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Laboratory of Pathology, Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Biliary Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Pages: 588-592
|
Published online on: December 4, 2015

https://doi.org/10.3892/etm.2015.2914
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Abstract

Recognition of pathogen-associated molecular patterns by Toll-like receptors (TLRs) activates innate and adaptive immune responses. Among the 11 members of the human TLR family, TLR-5 is known to play an important role in the defense against bacterial invasion by binding to flagellin, a conserved component of bacteria. Previous studies have demonstrated that the activation of TLR‑5 induces the expression of interleukin (IL)‑10, IL‑12 and interferon‑β. However, the aim of the present study was to analyze the expression of a wider range of immune‑related molecules upon stimulation with a TLR‑5 agonist. Following isolation from healthy volunteers, peripheral blood mononuclear cells (PBMCs) were stimulated with flagellin, a TLR‑5 agonist. At 4 h after stimulation, quantitative polymerase chain reaction (PCR) and an antibody chip array were conducted to determine the mRNA expression levels of immune molecules and the protein secretion of immune molecules in the supernatant, respectively. The PCR results revealed that activation of TLR‑5 significantly influenced the expression of a number of important molecules. In addition, the antibody chip array demonstrated the induction (IL‑8) and inhibition [monocyte chemoattractant protein (MCP)‑1, MCP‑3 and macrophage inflammatory protein‑1α) of protein secretion following TLR‑5 stimulation. Therefore, the present study demonstrated the importance of TLR‑5 in regulating the biological function of PBMCs. In the future, research should focus on the roles of the candidate molecules in TLR‑5-mediating functions.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression levels of cytokines and chemokines increase in human peripheral blood mononuclear cells stimulated by activation of the Toll-like receptor 5 pathway

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