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Effect of local insulin injection on wound vascularization in patients with diabetic foot ulcer

  • Authors:
    • Zhaoxin Zhang
    • Lei Lv
  • View Affiliations / Copyright

    Affiliations: Department of Burns, People's Hospital of Xinjiang Uygur Autonomous Region, Urumchi, Xinjiang Uygur Autonomous Region 830001, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 397-402
    |
    Published online on: December 8, 2015
       https://doi.org/10.3892/etm.2015.2917
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Abstract

The aim of the present study was to investigate the effect of local insulin injection on granulation tissue formation in the wounds of patients with diabetic foot ulcer. Thirty‑two patients with diabetic foot ulcer were randomly divided into an insulin (n=18) and a control (n=14) group. In the diabetic foot ulcer wound, the insulin group were administered insulin and the control group were administered an equal volume of saline. Prior to injection and at 0.5, 1.0, 2.0 and 4.0 h after injection, the fingertip blood glucose levels were determined. The growth of granulation tissue was assessed continuously for 12 days. Wound tissue was harvested at 0, 5, 7 and 12 days for the detection of CD34 expression by immunohistochemistry. The microvessel density (MVD) was calculated. No significant difference in the fasting blood glucose level was found between the two groups at any time‑point (P>0.05). Growth of granulation tissue in the insulin group was more marked from 7 days after local insulin injection (24.87±0.24) and was significantly different from that in the control group (18.66±0.45) (P<0.01). New vessels were observed in the insulin group 3 days after insulin injection; however, there was no significant difference in MVD compared with the control group (P>0.05). The MVD in the insulin group increased markedly from 5 days after treatment, and the difference between the two groups was significant (P<0.01). In conclusion, local injection of insulin into the base of a diabetic foot ulcer has a significant effect on systemic blood glucose and may promote wound healing by improving the growth of granulation tissue.
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1 

Aalaa M, Malazy OT, Sanjari M, Peimani M and Mohajeri-Tehrani M: Nurses' role in diabetic foot prevention and care; a review. J Diabetes Metab Disord. 11:242012. View Article : Google Scholar : PubMed/NCBI

2 

Alavi A, Sibbald RG, Mayer D, Goodman L, Botros M, Armstrong DG, Woo K, Boeni T, Ayello EA and Kirsner RS: Diabetic foot ulcers, Part II. Histopathology. J Am Acad Dermatol. 70:212014. View Article : Google Scholar : PubMed/NCBI

3 

Madibally SV, Solomon V and Mitchell RN: VanD e Water L, Yarmush ML and Toner M: Influence of insulin therapy on burn wound healing in rats. J Surg Res. 109:92–100. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Lima MH, Caricilli AM, de Abreu LL, Araújo EP, Pelegrinelli FF, Thirone AC, Tsukumo DM, Pessoa AF, dos Santos MF, de Moraes MA, et al: Topical insulin accelerates wound healing in diabetes by enhancing the AKT and ERK pathways: A double-blind placebo-controlled clinical trial. PLoS One. 7:e369742012. View Article : Google Scholar : PubMed/NCBI

5 

Martínez-Jiménez MA, Aguilar-García J, Valdés-Rodríguez R, Metlich-Medlich MA, Dietsch LJ, Gaitán-Gaona FI, Kolosovas-Machuca ES, González FJ and Sánchez-Aguilar JM: Local use of insulin in wounds with diabetic patients Higher temperature, fibrosis, and angiogenesis. Plast Reconstr Surg. 32:1015e–1019e. 2013.

6 

American Diabetes: Association: S tandards of medical care in diabetes - 2010. Diabetes Care. 33((Suppl 1)): S11–S61. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Zhang XJ and Chinkes DL: SadagopaR amanujam VM and Wolfe RR: Local injection of insulin-zinc stimulates DNA synthesis in skin donor site wound. Wound Repair Regen. 15:258–265. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Zhang XJ, Wu X, Wolf SE, Hawkins HK, Chinkes DL and Wolfe RR: Local insulin-zinc injection accelerates skin donor site wound healing. J Surg Res. 142:90–96. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Liu Y, Zhang X, Zhang Z, Fang PY and Xu WS: Effects of topical application of insulin on the wound healing in scalded rats. Zhonghua Shao Shang Za Zhi. 20:98–101. 2004.(In Chinese). PubMed/NCBI

10 

Liu Y, Zhang X, Zhang Z and Xu WS: The influence of topical application of insulin on the formation of basement membrane in scalded rats. Zhonghua Shao Shang Za Zhi. 21:445–447. 2005.(In Chinese). PubMed/NCBI

11 

Schramm JC, Dinh T and Veves A: Microvascular change in the diabetic foot. Int J Low Extrem Wounds. 5:149–159. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Martin A, Komada MR and Sane DC: Abnormal angiogenesis in diabetes mellitus. Med Res Rev. 23:117–145. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Cho CH, Sung HK, Kim KT, Cheon HG, Oh GT, Hong HJ, Yoo OJ and Koh GY: COMP-angiopoietin-1 promotes wound healing through enhanced angiogenesis, lymphangiogenesis and blood flow in a diabetic mouse model. Proc Natl Acad Sci USA. 103:4946–4951. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Pareek G, Shevchuk M, Armenakes NA, Vasjovic L, Hochberg DA, Basillote JB and Fracchia JA: The effect of finasteride on the expression of vascular endothelial growth factor and microvessel density, A possible mechanism for decreased prostatic bleeding in treated patients. J Urol. 169:20–23. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Li DL, Li QX and Peng DZ: Wound size measurement using digital camera and Photoshop software. Zhong Hua Shao Shang Za Zhi. 26:482010.(In Chinese).

16 

Dandona P, Mohanty P, Chaudhuri A, Garg R and Aljada A: Insulin infusion in acute illness. J Clin Invest. 115:2069–2072. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Langouche L, Vanhorebeek I, Vlasselaers D, Vander Perre S, Wouters PJ, Skogstrand K, Hansen TK and Van den Berghe G: Intensive insulin therapy protects the endothelium of critically ill patients. J Clin Invest. 115:2277–2286. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Zhang XJ, Meng C, Chinkes DL and Herndon DN: Beneficial effects of insulin on cell proliferation and protein metabolism in skin donor site wound. J Surg Res. 168:e155–e161. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Adam KM, Mahmoud SM, Mahadi SI, Widatalla AH, Shawer MA and Ahmed ME: Extended leg infection of diabetic foot ulcers, Risk factors and outcome. J Wound Care. 20:440–444. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Abolfotouh MA, Alfaifi SA and Al-Gannas AS: Risk factors of diabetic foot in central Saudi Arabia. Saudi Med J. 132:708–713. 2011.

21 

Zhang ZX, Liu XL, Lü L, Zhang L, Ji DL and Liu LH: Effect of insulin by local injection on the level of systemic blood glucose and granulation tissue formation of wound in patients with diabetic foot ulcer. Zhonghua Shao Shang Za Zhi. 27:451–455. 2011.(In Chinese). PubMed/NCBI

22 

Sinwar PD: The diabetic foot management - recent advance. Int J Surg. 15:27–30. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Dandona P, Aljada A, Mohanty P, Ghanim H, Hamouda W, Assian E and Ahmad S: Insulin inhibits intranuclear nuclear factor kappaB and stimulates IkappaB in mononuclear cells in obese subjects, Evidence for an anti-inflammatory effect? J Clin Endocrinol Metab. 86:3257–3265. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Aljada A, Ghanim H, Mohanty P, Kapur N and Dandona P: Insulin inhibits the pro-inflammatory transcription factor early growth response gene-1 (Egr)-1 expression in mononuclear cells (MNC) and reduces plasma tissue factor (TF) and plasminogen activator inhibitor-1 (PAI-1) concentrations. J Clin Endocrinol Metab. 87:1419–1422. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Chaudhuri A, Janicke D, Wilson MF, Tripathy D, Garg R, Bandyopadhyay A, Calieri J, Hoffmeyer D, Syed T, Ghanim H, et al: Anti-inflammatory and profibrinolytic effect of insulin in acute ST-segment-elevation myocardial infarction. Circulation. 109:849–854. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Aljada A, Ghanim H, Mohanty P, Syed T, Bandyopadhyay A and Dandona P: Glucose intake induces an increase in activator protein 1 and early growth response 1 binding activities, in the expression of tissue factor and matrix metalloproteinase in mononuclear cells, and in plasma tissue factor and matrix metalloproteinase concentrations. Am J Clin Nutr. 80:51–57. 2004.PubMed/NCBI

27 

Wang L, Zhao X, Wei BY, Liu Y, Ma XY, Wang J, Cao PC, Zhang Y, Yan YB, Lei W and Feng YF: Insulin improves osteogenesis of titanium implants under diabetic conditions by inhibiting reactive oxygen species overproduction via the PI3K-Akt pathway. Biochimie. 108:85–93. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Kimball SR, Vary TC and Jeferson LS: Regulation of protein synthesis by insulin. Annu Rev Physiol. 56:321–348. 1994. View Article : Google Scholar : PubMed/NCBI

29 

Papapetropoulos A, García-Cardeña G, Madri JA and Sessa WC: Nitric oxide production contributes to the angiogenic properties of vascular endothelial growth factor in human endothelial cells. J Clin Invest. 100:3131–3139. 1997. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z and Lv L: Effect of local insulin injection on wound vascularization in patients with diabetic foot ulcer. Exp Ther Med 11: 397-402, 2016.
APA
Zhang, Z., & Lv, L. (2016). Effect of local insulin injection on wound vascularization in patients with diabetic foot ulcer. Experimental and Therapeutic Medicine, 11, 397-402. https://doi.org/10.3892/etm.2015.2917
MLA
Zhang, Z., Lv, L."Effect of local insulin injection on wound vascularization in patients with diabetic foot ulcer". Experimental and Therapeutic Medicine 11.2 (2016): 397-402.
Chicago
Zhang, Z., Lv, L."Effect of local insulin injection on wound vascularization in patients with diabetic foot ulcer". Experimental and Therapeutic Medicine 11, no. 2 (2016): 397-402. https://doi.org/10.3892/etm.2015.2917
Copy and paste a formatted citation
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Spandidos Publications style
Zhang Z and Lv L: Effect of local insulin injection on wound vascularization in patients with diabetic foot ulcer. Exp Ther Med 11: 397-402, 2016.
APA
Zhang, Z., & Lv, L. (2016). Effect of local insulin injection on wound vascularization in patients with diabetic foot ulcer. Experimental and Therapeutic Medicine, 11, 397-402. https://doi.org/10.3892/etm.2015.2917
MLA
Zhang, Z., Lv, L."Effect of local insulin injection on wound vascularization in patients with diabetic foot ulcer". Experimental and Therapeutic Medicine 11.2 (2016): 397-402.
Chicago
Zhang, Z., Lv, L."Effect of local insulin injection on wound vascularization in patients with diabetic foot ulcer". Experimental and Therapeutic Medicine 11, no. 2 (2016): 397-402. https://doi.org/10.3892/etm.2015.2917
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