Effects of erythropoietin preconditioning on rat cerebral ischemia‑reperfusion injury and the GLT‑1/GLAST pathway

  • Authors:
    • Daihua Yu
    • Yuanhua Fan
    • Xude Sun
    • Linong Yao
    • Wei Chai
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  • Published online on: December 8, 2015     https://doi.org/10.3892/etm.2015.2919
  • Pages: 513-518
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Abstract

The aim of the present study was to investigate whether erythropoietin (EPO) preconditioning affects the expression of glutamate transporter 1 (GLT‑1) and glutamate aspartate transporter (GLAST) and protects against rat cerebral ischemia‑reperfusion injury. A total of 140 Sprague Dawley rats were randomly assigned to one of the following four groups: Sham, EPO‑sham, middle cerebral artery occlusion (MCAO) and EPO‑MCAO. Neurological function scores were obtained 24, 36 and 72 h after reperfusion. Seventy‑two hours after the induction of cerebral ischemia‑reperfusion, the number of apoptotic neural cells and the cerebral infarct volume of each group were measured. The mRNA levels of GLT‑1 and GLAST were determined using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis, while the GLT‑1 and GLAST protein levels were assessed using western blotting. The cerebral infarct volume was significantly increased in the MCAO group compared with that in the sham group (P<0.01); however, the infarct volume of the EPO‑MCAO group was significantly lower than that of the MCAO group (P<0.01). In addition, the number of apoptotic cells found in the MCAO group was higher than that in the sham group (P<0.01), but the number of apoptotic cells in the EPO‑MCAO group was significantly lower than that in the MCAO group (P<0.01). The GLT‑1 and GLAST mRNA and protein levels were significantly decreased 72 h after the cerebral ischemia‑reperfusion (P<0.01) compared with those in the sham group, whereas the same levels were increased significantly in the EPO‑MCAO group relative to those in the MCAO group (P<0.01). In conclusion, EPO preconditioning protected against cerebral ischemia‑reperfusion injury and upregulated the GLT‑1 and GLAST expression.
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February-2016
Volume 11 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yu D, Fan Y, Sun X, Yao L and Chai W: Effects of erythropoietin preconditioning on rat cerebral ischemia‑reperfusion injury and the GLT‑1/GLAST pathway. Exp Ther Med 11: 513-518, 2016
APA
Yu, D., Fan, Y., Sun, X., Yao, L., & Chai, W. (2016). Effects of erythropoietin preconditioning on rat cerebral ischemia‑reperfusion injury and the GLT‑1/GLAST pathway. Experimental and Therapeutic Medicine, 11, 513-518. https://doi.org/10.3892/etm.2015.2919
MLA
Yu, D., Fan, Y., Sun, X., Yao, L., Chai, W."Effects of erythropoietin preconditioning on rat cerebral ischemia‑reperfusion injury and the GLT‑1/GLAST pathway". Experimental and Therapeutic Medicine 11.2 (2016): 513-518.
Chicago
Yu, D., Fan, Y., Sun, X., Yao, L., Chai, W."Effects of erythropoietin preconditioning on rat cerebral ischemia‑reperfusion injury and the GLT‑1/GLAST pathway". Experimental and Therapeutic Medicine 11, no. 2 (2016): 513-518. https://doi.org/10.3892/etm.2015.2919