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Article

Small molecule compounds alleviate anisomycin‑induced oxidative stress injury in SH‑SY5Y cells via downregulation of p66shc and Aβ1‑42 expression

  • Authors:
    • Yun Wang
    • Te Liu
    • Weidong Pan
    • Huiying Chi
    • Jiulin Chen
    • Zhihua Yu
    • Chuan Chen
  • View Affiliations / Copyright

    Affiliations: Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200031, P.R. China, Department of Neurology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China
  • Pages: 593-600
    |
    Published online on: December 8, 2015
       https://doi.org/10.3892/etm.2015.2921
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Abstract

Oxidative stress and ageing are important factors contributing to the pathogenesis of Alzheimer's disease (AD), which is associated with neuronal damage and β‑amyloid (Aβ) deposition. The p66shc adaptor protein is important for the regulation of oxidative stress and ageing. In the present study, SH‑SY5Y human neuroblastoma cells were treated with anisomycin in order to establish a cell model of oxidative stress‑induced neuronal damage. The results from quantitative polymerase chain reaction, enzyme‑linked immunosorbent assay and western blotting demonstrated that anisomycin was able to stimulate the secretion of Aβ1‑42 from SH‑SY5Y cells and upregulate the expression levels of p66shc, which was associated with concomitant damage to SH‑SY5Y cells. In addition, the protective effects of various small molecule compounds with antioxidant properties against neuronal damage were evaluated. Notably, treatment of SH‑SY5Y cells with gallic acid was associated with significant downregulation of p66shc protein expression levels, reduced anisomycin‑induced secretion of Aβ1‑42, and increased superoxide dismutase activity and acetylcholine secretion levels. The results of the present study suggested that anisomycin is able to promote oxidative neuronal damage by inducing the secretion of Aβ1‑42 from neurons and increasing the neuronal expression of p66shc, and this damage may be attenuated by treatment with gallic acid. Therefore, gallic acid and similar small molecule compounds may be considered for the alleviation of neuronal oxidative stress injury in patients with AD.
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1 

Götz J, Deters N, Doldissen A, Bokhari L, Ke Y, Wiesner A, Schonrock N and Ittner LM: A decade of tau transgenic animal models and beyond. Brain Pathol. 17:91–103. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Mattson MP: Pathways towards and away fromA lzheimer's disease. Nature. 430:631–639. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Minter MR, Taylor JM and Crack PJ: The contribution of neuro-inflammation to amyloid toxicity in Alzheimer's disease. J Neurochem Oct. 28:2015.(Epub ahead of print).

4 

Wimo A: Long-term effects of A lzheimer's disease treatment. Lancet Neurol. doi: http://dx.doi.org/10.1016/S1474-4422(15)00302-6. PubMed/NCBI

5 

Mawuenyega KG, Sigurdson W, Ovod V, Munsell L, Kasten T, Morris JC, Yarasheski KE and Bateman RJ: Decreased clearance of CNS beta-amyloid in Alzheimer's disease. Science. 330:17742010. View Article : Google Scholar : PubMed/NCBI

6 

Migliaccio E, Giorgio M, Mele S, Pelicci G, Reboldi P, Pandolfi PP, Lanfrancone L and Pelicci PG: The p66shc adaptor protein controls oxidative stress response and life span in mammals. Nature. 402:309–313. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Shan W, Gao L, Zeng W, Hu Y, Wang G, Li M, Zhou J, Ma X, Tian X and Yao J: Activation of the SIRT1/p66shc antiapoptosis pathway via carnosic acid-induced inhibition of miR-34a protects rats against nonalcoholic fatty liver disease. Cell Death Dis. 6:e18332015. View Article : Google Scholar : PubMed/NCBI

8 

Priami C, De Michele G, Cotelli F, Cellerino A, Giorgio M, Pelicci PG and Migliaccio E: Modelling the p53/p66Shc Aging Pathway in the Shortest Living Vertebrate Nothobranchius Furzeri. Aging Dis. 6:95–108. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Li X, Gao D, Wang H, Li X, Yang J, Yan X, Liu Z and Ma Z: Negative feedback loop between p66Shc and ZEB1 regulates fibrotic EMT response in lung cancer cells. Cell Death Dis. 6:e17082015. View Article : Google Scholar : PubMed/NCBI

10 

Bhat SS, Anand D and Khanday FA: p66Shc as a switch in bringing about contrasting responses in cell growth, Implications on cell proliferation and apoptosis. Mol Cancer. 14:762015. View Article : Google Scholar : PubMed/NCBI

11 

Perrini S, Tortosa F, Natalicchio A, Pacelli C, Cignarelli A, Palmieri VO, Caccioppoli C, De Stefano F, Porro S, Leonardini A, et al: The p66Shc Protein Controls Redox Signaling and Oxidation-Dependent DNA Damage in Human Liver Cells. Am J Physiol Gastrointest Liver Physiol: Sep. 3:2015.(Epub ahead of print).

12 

Sampaio SF, Branco AF, Wojtala A, Vega-Naredo I, Wieckowski MR and Oliveira PJ: p66Shc signaling is involved in stress responses elicited by anthracycline treatment of rat cardiomyoblasts. Arch Toxicol Aug. 30:2015.(Epub ahead of print).

13 

Guo X, Wu X, Ren L, Liu G and Li L: Epigenetic mechanisms of amyloid-β production in anisomycin-treated SH-SY5Y cells. Neuroscience. 194:272–281. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Pena RR, Pereira-Caixeta AR, Moraes MF and Pereira GS: Anisomycin administered in the olfactory bulb and dorsal hippocampus impaired social recognition memory consolidation in different time-points. Brain Res Bull. 109:151–157. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Sorg BA, Todd RP, Slaker M and Churchill L: Anisomycin in the medial prefrontal cortex reduces reconsolidation of cocaine-associated memories in the rat self-administration model. Neuropharmacology. 92:25–33. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Dubue JD, McKinney TL, Treit D and Dickson CT: Intrahippocampal Anisomycin Impairs Spatial Performance on the Morris Water Maze. J Neurosci. 35:11118–11124. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Hardy J and Selkoe DJ: The amyloid hypothesis of Alzheimer's disease: Progress and problems on the road to therapeutics. Science. 297:353–356. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Zou G, Du X, Duan T and Liu T: Application of a NotI subtraction and methylation specific genome subtractive hybridization technique in the detection of genomic DNA methylation differences between hydatidiform moles and villi. Mol Med Rep. 7:77–82. 2013.PubMed/NCBI

19 

Chen Q, Qiu C, Huang Y, Jiang L, Huang Q, Guo L and Liu T: Human amniotic epithelial cell feeder layers maintain iPS cell pluripotency by inhibiting endogenous DNA methyltransferase 1. Exp Ther Med. 6:1145–1154. 2013.PubMed/NCBI

20 

Berry A, Greco A, Giorgio M, Pelicci PG, de Kloet R, Alleva E, Minghetti L and Cirulli F: Deletion of the lifespan determinant p66(Shc) improves performance in a spatial memory task, decreases levels of oxidative stress markers in the hippocampus and increases levels of the neurotrophin BDNF in adult mice. Exp Gerontol. 43:200–208. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Berry A, Capone F, Giorgio M, Pelicci PG, de Kloet ER, Alleva E, Minghetti L and Cirulli F: Deletion of the life span determinant p66Shc prevents age-dependent increases in emotionality and pain sensitivity in mice. Exp Gerontol. 42:37–45. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Berry A and Cirulli F: The p66(Shc) gene paves the way for healthspan: Evolutionary and mechanistic perspectives. Neurosci Biobehav Rev. 37:790–802. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Sone K, Mori M and Mori N: Selective upregulation of p66-Shc gene expression in the liver and brain of aged rats. Arch Gerontol Geriatr. 55:744–748. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Liu T, Pan W, Chi H, Chen J, Yu Z and Chen C: Small molecule compounds alleviate anisomycin‑induced oxidative stress injury in SH‑SY5Y cells via downregulation of p66shc and Aβ1‑42 expression. Exp Ther Med 11: 593-600, 2016.
APA
Wang, Y., Liu, T., Pan, W., Chi, H., Chen, J., Yu, Z., & Chen, C. (2016). Small molecule compounds alleviate anisomycin‑induced oxidative stress injury in SH‑SY5Y cells via downregulation of p66shc and Aβ1‑42 expression. Experimental and Therapeutic Medicine, 11, 593-600. https://doi.org/10.3892/etm.2015.2921
MLA
Wang, Y., Liu, T., Pan, W., Chi, H., Chen, J., Yu, Z., Chen, C."Small molecule compounds alleviate anisomycin‑induced oxidative stress injury in SH‑SY5Y cells via downregulation of p66shc and Aβ1‑42 expression". Experimental and Therapeutic Medicine 11.2 (2016): 593-600.
Chicago
Wang, Y., Liu, T., Pan, W., Chi, H., Chen, J., Yu, Z., Chen, C."Small molecule compounds alleviate anisomycin‑induced oxidative stress injury in SH‑SY5Y cells via downregulation of p66shc and Aβ1‑42 expression". Experimental and Therapeutic Medicine 11, no. 2 (2016): 593-600. https://doi.org/10.3892/etm.2015.2921
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Liu T, Pan W, Chi H, Chen J, Yu Z and Chen C: Small molecule compounds alleviate anisomycin‑induced oxidative stress injury in SH‑SY5Y cells via downregulation of p66shc and Aβ1‑42 expression. Exp Ther Med 11: 593-600, 2016.
APA
Wang, Y., Liu, T., Pan, W., Chi, H., Chen, J., Yu, Z., & Chen, C. (2016). Small molecule compounds alleviate anisomycin‑induced oxidative stress injury in SH‑SY5Y cells via downregulation of p66shc and Aβ1‑42 expression. Experimental and Therapeutic Medicine, 11, 593-600. https://doi.org/10.3892/etm.2015.2921
MLA
Wang, Y., Liu, T., Pan, W., Chi, H., Chen, J., Yu, Z., Chen, C."Small molecule compounds alleviate anisomycin‑induced oxidative stress injury in SH‑SY5Y cells via downregulation of p66shc and Aβ1‑42 expression". Experimental and Therapeutic Medicine 11.2 (2016): 593-600.
Chicago
Wang, Y., Liu, T., Pan, W., Chi, H., Chen, J., Yu, Z., Chen, C."Small molecule compounds alleviate anisomycin‑induced oxidative stress injury in SH‑SY5Y cells via downregulation of p66shc and Aβ1‑42 expression". Experimental and Therapeutic Medicine 11, no. 2 (2016): 593-600. https://doi.org/10.3892/etm.2015.2921
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