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Article

Protein kinase C-β inhibitor treatment attenuates hepatic ischemia and reperfusion injury in diabetic rats

  • Authors:
    • Guang‑Xing Meng
    • Qiang Yuan
    • Li‑Ping Wei
    • Hua Meng
    • Yi‑Jun Wang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Tianjin Hepatobiliary Research Institute, Tianjin Third Central Hospital, Tianjin 300170, P.R. China, Department of Cardiology, Tianjin Union Medicine Center, Tianjin 300121, P.R. China, Department of Gastrointestinal Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China
  • Pages: 565-570
    |
    Published online on: December 9, 2015
       https://doi.org/10.3892/etm.2015.2927
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Abstract

Hepatic ischemia and reperfusion (I/R) injury plays an active role in hepatic resection and transplantation. While the effects of protein kinase C (PKC)‑βII activation and the role of PKC‑β inhibitors are well understood in myocardial I/R in diabetes, they remain unclear in liver I/R. The aim of this study was to explore the effect of PKC‑β inhibition and the potential mechanism by which PKC‑β inhibitor treatment protects against hepatic I/R injury in diabetic rats. Diabetic rats were established and randomized into two groups. These were an untreated group (n=10), which did not receive any treatment, and a treatment group (n=10), orally treated with ruboxistaurin at a dose of 5 mg/kg/day for 2 weeks. The rats from the two groups were subjected to hepatic I/R. Aspartate transaminase (AST) and lactate dehydrogenase (LDH) levels were measured by enzymatic methods at 1, 3 and 5 h after I/R. Tumor necrosis factor‑α (TNF‑α) and intercellular adhesion molecule 1 (ICAM‑1) were examined by enzyme‑linked immunosorbent assay at the same time‑points. Nuclear factor‑κB (NF‑κB) p65 expression was analyzed by immunofluorescence and western blotting. Apoptosis of hepatic cells was examined by the western blot analysis of caspase 3 expression and by DNA ladder analysis. Pathological changes were examined using light and electron microscopy. Serum AST and LDH levels in the PKC‑β inhibitor treatment group were diminished compared with those in the untreated group (P<0.01). Serum TNF‑α and ICAM‑1 (P<0.01) levels were also decreased at different time‑points in the PKC‑β inhibitor treatment group. The relative expression of NF‑κB p65 and caspase 3 in the hepatic tissue was weakened in the PKC‑β inhibitor treatment group compared with that in the untreated group (P<0.01). Pathological changes in hepatic tissue were attenuated by the PKC‑β inhibitor. In conclusion, PKC‑β inhibitor treatment protected against liver I/R injury in diabetic rats. The mechanisms probably involved the attenuation of microvascular injury, reduced transport of injury‑associated factors and diminishment of the activation of NF‑κB p65.
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1 

Rahbari NN, Reissfelder C, Koch M, et al: The predictive value of postoperative clinical risk scores for outcome after hepatic resection: a validation analysis in 807 patients. Ann Surg Oncol. 18:3640–3649. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Karatzas T, Neri AA, Baibaki ME and Dontas IA: Rodent models of hepatic ischemia-reperfusion injury, time and percentage-related pathophysiological mechanisms. J Surg Res. 191:399–412. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Rahbari NN, Garden OJ, Padbury R, et al: Posthepatectomy liver failure: a definition and grading by the International Study Group of Liver Surgery (ISGLS). Surgery. 149:713–724. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Schmidt R: Hepatic organ protection: from basic science to clinical practice. World J Gastroenterol. 16:6044–6045. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Su AP, Cao SS, Le Tian B, et al: Effect of transjugular intrahepatic portosystemic shunt on glycometabolism in cirrhosis patients. Clin Res Hepatol Gastroenterol. 36:53–59. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Çekın AH, Gür G, Türkoğlu S, et al: The protective effect of L-carnitine on hepatic ischemia-reperfusion injury in rats. Turk J Gastroenterol. 24:51–56. 2013.PubMed/NCBI

7 

Yang J, Sun H, Takacs P, et al: The effect of octreotide on hepatic ischemia-reperfusion injury in a rabbit model. Transplant Proc. 45:2433–2438. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Jin LM, Liu YX, Zhou L, et al: Ischemic preconditioning attenuates morphological and biochemical changes in hepatic ischemia/reperfusion in rats. Pathobiology. 77:136–146. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Clarke M and Dodson PM: PKC inhibition and diabetic microvascular complications. Best Pract Res Clin Endocrinol Metab. 21:573–586. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Wei L, Yin Z, Yuan Y, et al: A PKC-beta inhibitor treatment reverses cardiac microvascular barrier dysfunction in diabetic rats. Microvasc Res. 80:158–165. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Budhiraja S and Singh J: Protein kinase C beta inhibitors: a new therapeutic target for diabetic nephropathy and vascular complications. Fundam Clinical Pharmacol. 22:231–240. 2008. View Article : Google Scholar

12 

Wei L, Sun D, Yin Z, et al: A PKC-beta inhibitor protects against cardiac microvascular ischemia reperfusion injury in diabetic rats. Apoptosis. 15:488–498. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Yin Z, Fan L, Wei L, et al: FTY720 protects cardiac microvessels of diabetes: a critical role of S1P1/3 in diabetic heart disease. PLoS One. 7:e429002012. View Article : Google Scholar : PubMed/NCBI

14 

Goldenberger D, Perschil I, Ritzler M and Altwegg M: A simple “universal” DNA extraction procedure using SDS and proteinase K is compatible with direct PCR amplification. PCR Methods Appl. 4:368–370. 1995. View Article : Google Scholar : PubMed/NCBI

15 

Gutterman DD: Vascular dysfunction in hyperglycemia: is protein kinase. C the culprit? Circ Res. 90:5–7. 2002.PubMed/NCBI

16 

Geraldes P and King GL: Activation of protein kinase C isoforms and its impact on diabetic complications. Circ Res. 106:1319–1331. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Joy SV, Scates AC, Bearelly S, et al: Ruboxistaurin, a protein kinase C beta inhibitor, as an emerging treatment for diabetes microvascular complications. Ann Pharmacother. 39:1693–1699. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Galloway E, Shin T, Huber N, et al: Activation of hepatocytes by extracellular heat shock protein 72. Am J Physiol Cell Physiol. 295:C514–C520. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Wang H, Li ZY, Wu HS, et al: Endogenous danger signals trigger hepatic ischemia/reperfusion injury through toll-like receptor 4/nuclear factor-kappa B pathway. Chin Med J (Engl). 120:509–514. 2007.PubMed/NCBI

20 

Zhang W, An J, Jawadi H, et al: Sphingosine-1-phosphate receptor-2 mediated NFκB activation contributes to tumor necrosis factor-α induced VCAM-1 and ICAM-1 expression in endothelial cells. Prostaglandins Other Lipid Mediat. 106:62–71. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Zhu YP, Shen T, Lin YJ, et al: Astragalus polysaccharides suppress ICAM-1 and VCAM-1 expression in TNF-α-treated human vascular endothelial cells by blocking NF-κB activation. Acta Pharmacol Sin. 34:1036–1042. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Chen X, Ding WX, Ni HM, et al: Bid-independent mitochondrial activation in tumor necrosis factor alpha-induced apoptosis and liver injury. Mol Cell Biol. 27:541–553. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Hatano E: Tumor necrosis factor signaling in hepatocyte apoptosis. J Gastroenterol Hepatol. 22((Suppl 1)): S43–S44. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Martin J, Romanque P, Maurhofer O, et al: Ablation of the tumor suppressor histidine triad nucleotide binding protein 1 is protective against hepatic ischemia/reperfusion injury. Hepatology. 53:243–252. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Giakoustidis DE, Giakoustidis AE, Iliadis S, et al: Attenuation of liver ischemia/reperfusion induced apoptosis by epigallocatechin-3-gallate via down-regulation of NF-kappaB and c-Jun expression. J Surg Res. 159:720–728. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Huet PM, Nagaoka MR, Desbiens G, et al: Sinusoidal endothelial cell and hepatocyte death following cold ischemia-warm reperfusion of the rat liver. Hepatology. 39:1110–1119. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Teoh NC and Farrell GC: Hepatic ischemia reperfusion injury: pathogenic mechanisms and basis for hepatoprotection. J Gastroenterol Hepatol. 18:891–902. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Guo JY, Yang T, Sun XG, et al: Ischemic postconditioning attenuates liver warm ischemia-reperfusion injury through Akt-eNOS-NO-HIF pathway. J Biomed Sci. 18:792011. View Article : Google Scholar : PubMed/NCBI

29 

Taki-Eldin A, Zhou L, Xie HY, et al: Triiodothyronine attenuates hepatic ischemia/reperfusion injury in a partial hepatectomy model through inhibition of proinflammatory cytokines, transcription factors, and adhesion molecules. J Surg Res. 178:646–656. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Coito AJ: Leukocyte transmigration across endothelial and extracellular matrix protein barriers in liver ischemia/reperfusion injury. Curr Opin Organ Transplant. 16:34–40. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Xu J, Xie J, Bao M, et al: NF-kappaB/I-kappaB pathway during ischemia reperfusion injury of rat liver. Chin Med J (Engl). 116:1146–1149. 2003.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Meng GX, Yuan Q, Wei LP, Meng H and Wang YJ: Protein kinase C-β inhibitor treatment attenuates hepatic ischemia and reperfusion injury in diabetic rats. Exp Ther Med 11: 565-570, 2016.
APA
Meng, G., Yuan, Q., Wei, L., Meng, H., & Wang, Y. (2016). Protein kinase C-β inhibitor treatment attenuates hepatic ischemia and reperfusion injury in diabetic rats. Experimental and Therapeutic Medicine, 11, 565-570. https://doi.org/10.3892/etm.2015.2927
MLA
Meng, G., Yuan, Q., Wei, L., Meng, H., Wang, Y."Protein kinase C-β inhibitor treatment attenuates hepatic ischemia and reperfusion injury in diabetic rats". Experimental and Therapeutic Medicine 11.2 (2016): 565-570.
Chicago
Meng, G., Yuan, Q., Wei, L., Meng, H., Wang, Y."Protein kinase C-β inhibitor treatment attenuates hepatic ischemia and reperfusion injury in diabetic rats". Experimental and Therapeutic Medicine 11, no. 2 (2016): 565-570. https://doi.org/10.3892/etm.2015.2927
Copy and paste a formatted citation
x
Spandidos Publications style
Meng GX, Yuan Q, Wei LP, Meng H and Wang YJ: Protein kinase C-β inhibitor treatment attenuates hepatic ischemia and reperfusion injury in diabetic rats. Exp Ther Med 11: 565-570, 2016.
APA
Meng, G., Yuan, Q., Wei, L., Meng, H., & Wang, Y. (2016). Protein kinase C-β inhibitor treatment attenuates hepatic ischemia and reperfusion injury in diabetic rats. Experimental and Therapeutic Medicine, 11, 565-570. https://doi.org/10.3892/etm.2015.2927
MLA
Meng, G., Yuan, Q., Wei, L., Meng, H., Wang, Y."Protein kinase C-β inhibitor treatment attenuates hepatic ischemia and reperfusion injury in diabetic rats". Experimental and Therapeutic Medicine 11.2 (2016): 565-570.
Chicago
Meng, G., Yuan, Q., Wei, L., Meng, H., Wang, Y."Protein kinase C-β inhibitor treatment attenuates hepatic ischemia and reperfusion injury in diabetic rats". Experimental and Therapeutic Medicine 11, no. 2 (2016): 565-570. https://doi.org/10.3892/etm.2015.2927
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