Open Access

Biological characterization of metanephric mesenchymal stem cells from the Beijing duck

  • Authors:
    • Jia Chen
    • Yabin Pu
    • Yujiao Sun
    • Ping Zhang
    • Qian Li
    • Kunfu Wang
    • Wenjie Wang
    • Yuehui Ma
    • Weijun Guan
  • View Affiliations

  • Published online on: December 16, 2015     https://doi.org/10.3892/etm.2015.2943
  • Pages: 439-447
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mesenchymal stem cells (MSCs) possess self‑proliferation and multi‑directional differentiation abilities. Previous studies on MSCs have mostly focused on the bone marrow, lungs, pancreas and umbilical cord blood, with few studies on metanephric tissues in ducks. For the present study, the Beijing duck was selected as an experimental animal. Duck embryo metanephric mesenchymal stem cells (MMSCs) were studied. MMSC isolation culture, analysis of biological characteristics, induced differentiation and identification were performed in preliminary experiments. In the current study, surface antigens and gene expression patterns were detected using immunofluorescence, reverse transcription‑polymerase chain reaction (RT‑PCR) and flow cytometry. The induced cells, adipocytes, hepatocytes, epithelial cells and islet cells were identified by oil red O staining, periodic acid‑Schiff staining, immunofluorescence and dithizone staining, respectively. RT‑PCR was performed for detection of specific marker genes. The results suggested that the biological characteristics of MMSCs were similar to those of the MSCs previously analyzed. Primary MMSCs were sub‑cultured to passage 21. The induced cells exhibit typical staining and immunofluorescence indicating the expression of specific genes. This demonstrates that MMSCs may be a novel alternative source of MSCs for experimental and clinical applications.
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February-2016
Volume 11 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen J, Pu Y, Sun Y, Zhang P, Li Q, Wang K, Wang W, Ma Y and Guan W: Biological characterization of metanephric mesenchymal stem cells from the Beijing duck. Exp Ther Med 11: 439-447, 2016
APA
Chen, J., Pu, Y., Sun, Y., Zhang, P., Li, Q., Wang, K. ... Guan, W. (2016). Biological characterization of metanephric mesenchymal stem cells from the Beijing duck. Experimental and Therapeutic Medicine, 11, 439-447. https://doi.org/10.3892/etm.2015.2943
MLA
Chen, J., Pu, Y., Sun, Y., Zhang, P., Li, Q., Wang, K., Wang, W., Ma, Y., Guan, W."Biological characterization of metanephric mesenchymal stem cells from the Beijing duck". Experimental and Therapeutic Medicine 11.2 (2016): 439-447.
Chicago
Chen, J., Pu, Y., Sun, Y., Zhang, P., Li, Q., Wang, K., Wang, W., Ma, Y., Guan, W."Biological characterization of metanephric mesenchymal stem cells from the Beijing duck". Experimental and Therapeutic Medicine 11, no. 2 (2016): 439-447. https://doi.org/10.3892/etm.2015.2943