Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model

  • Authors:
    • Yangbai Sun
    • Fengfeng Li
    • Cunyi Fan
  • View Affiliations

  • Published online on: December 16, 2015     https://doi.org/10.3892/etm.2015.2948
  • Pages: 547-552
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Abstract

Lentivirus (LV)‑mediated extracellular signal‑regulated kinase (ERK)2 small interfering RNA (siRNA) has previously been demonstrated to reduce post‑traumatic joint contractures: In the present study, the effect of ERK2 siRNA on extracellular matrix turnover within fibrotic joint capsules in post‑traumatic joint contractures was examined. Rats were randomly assigned to one of three groups as follows: The non‑operated control (CON), operated contracture (ORC) and contracture‑treatment (CNT) groups. Representative post‑traumatic joint contracture was created through 8 weeks of immobilization following intra‑articular injury. In the CNT group, LV‑mediated ERK2 siRNA was injected into the model knee at days 3 and 7 after surgery. The posterior joint capsule was examined by western blotting, histology and immunohistochemistry to evaluate alterations in ERK2, phosphorylated (p)ERK2, total collagen, collagen I, collagen III, matrix metalloproteinase (MMP)‑1, MMP‑13 and tissue inhibitor of metalloproteinase (TIMP)‑13. In the ORC group, pERK2 was elevated and total collagen, collagen I, MMP‑1 and MMP‑13 were significantly increased (P<0.01 vs. CON group); however, these were significantly decreased in the CNT group, and pERK2 was downregulated (P<0.01 vs. ORC group). Collagen III and TIMP‑13 were markedly decreased in the ORC group (P<0.01 vs. CON group), but elevated in the CNT group (P<0.01 vs. ORC group). The present res4ults demonstrate unique pathological changes of the fibrotic joint capsule that are responsible for joint contracture following traumatic injury, and reveal that extracellular matrix turnover can be affected by pERK2.
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February-2016
Volume 11 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Sun Y, Li F and Fan C: Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model. Exp Ther Med 11: 547-552, 2016
APA
Sun, Y., Li, F., & Fan, C. (2016). Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model. Experimental and Therapeutic Medicine, 11, 547-552. https://doi.org/10.3892/etm.2015.2948
MLA
Sun, Y., Li, F., Fan, C."Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model". Experimental and Therapeutic Medicine 11.2 (2016): 547-552.
Chicago
Sun, Y., Li, F., Fan, C."Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model". Experimental and Therapeutic Medicine 11, no. 2 (2016): 547-552. https://doi.org/10.3892/etm.2015.2948