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Article

Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model

  • Authors:
    • Yangbai Sun
    • Fengfeng Li
    • Cunyi Fan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
  • Pages: 547-552
    |
    Published online on: December 16, 2015
       https://doi.org/10.3892/etm.2015.2948
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Abstract

Lentivirus (LV)‑mediated extracellular signal‑regulated kinase (ERK)2 small interfering RNA (siRNA) has previously been demonstrated to reduce post‑traumatic joint contractures: In the present study, the effect of ERK2 siRNA on extracellular matrix turnover within fibrotic joint capsules in post‑traumatic joint contractures was examined. Rats were randomly assigned to one of three groups as follows: The non‑operated control (CON), operated contracture (ORC) and contracture‑treatment (CNT) groups. Representative post‑traumatic joint contracture was created through 8 weeks of immobilization following intra‑articular injury. In the CNT group, LV‑mediated ERK2 siRNA was injected into the model knee at days 3 and 7 after surgery. The posterior joint capsule was examined by western blotting, histology and immunohistochemistry to evaluate alterations in ERK2, phosphorylated (p)ERK2, total collagen, collagen I, collagen III, matrix metalloproteinase (MMP)‑1, MMP‑13 and tissue inhibitor of metalloproteinase (TIMP)‑13. In the ORC group, pERK2 was elevated and total collagen, collagen I, MMP‑1 and MMP‑13 were significantly increased (P<0.01 vs. CON group); however, these were significantly decreased in the CNT group, and pERK2 was downregulated (P<0.01 vs. ORC group). Collagen III and TIMP‑13 were markedly decreased in the ORC group (P<0.01 vs. CON group), but elevated in the CNT group (P<0.01 vs. ORC group). The present res4ults demonstrate unique pathological changes of the fibrotic joint capsule that are responsible for joint contracture following traumatic injury, and reveal that extracellular matrix turnover can be affected by pERK2.
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1 

Lindenhovius AL and Jupiter JB: The posttraumatic stiff elbow: A review of the literature. J Hand Surg Am. 32:1605–1623. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Gallay SH, Richards RR and O'Driscoll SW: Intraarticular capacity and compliance of stiff and normal elbows. Arthroscopy. 9:9–13. 1993. View Article : Google Scholar : PubMed/NCBI

3 

Cohen MS, Schimmel DR, Masuda K, Hastings H 2nd and Muehleman C: Structural and biochemical evaluation of the elbow capsule after trauma. J Shoulder Elbow Surg. 16:484–490. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Hildebrand KA, Zhang M, Germscheid NM, Wang C and Hart DA: Cellular, matrix, and growth factor components of the joint capsule are modified early in the process of posttraumatic contracture formation in a rabbit model. Acta Orthop. 79:116–125. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Monument MJ, Hart DA, Befus AD, Salo PT, Zhang M and Hildebrand KA: The mast cell stabilizer ketotifen reduces joint capsule fibrosis in a rabbit model of post-traumatic joint contractures. Inflamm Res. 61:285–292. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Monument MJ, Hart DA, Befus AD, Salo PT, Zhang M and Hildebrand KA: The mast cell stabilizer ketotifen fumarate lessens contracture severity and myofibroblast hyperplasia, A study of a rabbit model of posttraumatic joint contractures. J Bone Joint Surg Am. 92:1468–1477. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Li F, Liu S and Fan C: Lentivirus-mediated ERK2 siRNA reduces joint capsule fibrosis in a rat model of post-traumatic joint contracture. Int J Mol Sci. 14:20833–20844. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Hildebrand KA, Zhang M and Hart DA: Joint capsule matrix turnover in a rabbit model of chronic joint contractures, Correlation with human contractures. J Orthop Res. 24:1036–1043. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Akeson WH, Woo SL, Amiel D and Doty DH: Rapid recovery from contracture in rabbit hindlimb. A correlative biomechanical and biochemical study. Clin Orthop Relat Res. 359–365. 1977.PubMed/NCBI

10 

Finsterbush A and Friedman B: Reversibility of joint changes produced by immobilization in rabbits. Clin Orthop Relat Res. 290–298. 1975. View Article : Google Scholar : PubMed/NCBI

11 

Schollmeier G, Sarkar K, Fukuhara K and Uhthoff HK: Structural and functional changes in the canine shoulder after cessation of immobilization. Clin Orthop Relat Res. 310–315. 1996. View Article : Google Scholar : PubMed/NCBI

12 

Hildebrand KA, Zhang M, van Snellenberg W, King GJ and Hart DA: Myofibroblast numbers are elevated in human elbow capsules after trauma. Clin Orthop Relat Res. 189–197. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Abdel MP, Morrey ME, Barlow JD, Kreofsky CR, An KN, Steinmann SP, Morrey BF and Sanchez-Sotelo J: Myofibroblast cells are preferentially expressed early in a rabbit model of joint contracture. J Orthop Res. 30:713–719. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Hildebrand KA, Sutherland C and Zhang M: Rabbit knee model of post-traumatic joint contractures, The long-term natural history of motion loss and myofibroblasts. J Orthop Res. 22:313–320. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Germscheid NM and Hildebrand KA: Regional variation is present in elbow capsules after injury. Clin Orthop Relat Res. 450:219–224. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Fukui N, Tashiro T, Hiraoka H, Oda H and Nakamura K: Adhesion formation can be reduced by the suppression of transforming growth factor-beta1 activity. J Orthop Res. 18:212–219. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Fukui N, Nakajima K, Tashiro T, Oda H and Nakamura K: Neutralization of fibroblast growth factor-2 reduces intraarticular adhesions. Clin Orthop Relat Res. 250–258. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Fukui N, Fukuda A, Kojima K, Nakajima K, Oda H and Nakamura K: Suppression of fibrous adhesion by proteoglycan decorin. J Orthop Res. 19:456–462. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Hildebrand KA, Zhang M and Hart DA: High rate of joint capsule matrix turnover in chronic human elbow contractures. Clin Orthop Relat Res. 439:228–234. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Matsumoto F, Trudel G and Uhthoff HK: High collagen type I and low collagen type III levels in knee joint contracture, An immunohistochemical study with histological correlate. Acta Orthop Scand. 73:335–343. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Robins SP, Milne G, Duncan A, Davies C, Butt R, Greiling D and James IT: Increased skin collagen extractability and proportions of collagen type III are not normalized after 6 months healing of human excisional wounds. J Invest Dermatol. 121:267–272. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Sculean A, Junker R, Donos N, Berakdar M, Brecx M and Dünker N: Immunohistochemical evaluation of matrix molecules associated with wound healing following regenerative periodontal treatment in monkeys. Clin Oral Investig. 6:175–182. 2002.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun Y, Li F and Fan C: Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model. Exp Ther Med 11: 547-552, 2016.
APA
Sun, Y., Li, F., & Fan, C. (2016). Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model. Experimental and Therapeutic Medicine, 11, 547-552. https://doi.org/10.3892/etm.2015.2948
MLA
Sun, Y., Li, F., Fan, C."Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model". Experimental and Therapeutic Medicine 11.2 (2016): 547-552.
Chicago
Sun, Y., Li, F., Fan, C."Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model". Experimental and Therapeutic Medicine 11, no. 2 (2016): 547-552. https://doi.org/10.3892/etm.2015.2948
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Y, Li F and Fan C: Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model. Exp Ther Med 11: 547-552, 2016.
APA
Sun, Y., Li, F., & Fan, C. (2016). Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model. Experimental and Therapeutic Medicine, 11, 547-552. https://doi.org/10.3892/etm.2015.2948
MLA
Sun, Y., Li, F., Fan, C."Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model". Experimental and Therapeutic Medicine 11.2 (2016): 547-552.
Chicago
Sun, Y., Li, F., Fan, C."Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post‑traumatic joint contracture model". Experimental and Therapeutic Medicine 11, no. 2 (2016): 547-552. https://doi.org/10.3892/etm.2015.2948
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