Hydrogen sulfide improves neural function in rats following cardiopulmonary resuscitation

  • Authors:
    • Ji‑Yan Lin
    • Min‑Wei Zhang
    • Jin‑Gao Wang
    • Hui Li
    • Hong‑Yan Wei
    • Rong Liu
    • Gang Dai
    • Xiao‑Xing Liao
  • View Affiliations

  • Published online on: December 16, 2015     https://doi.org/10.3892/etm.2015.2950
  • Pages: 577-587
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The alleviation of brain injury is a key issue following cardiopulmonary resuscitation (CPR). Hydrogen sulfide (H2S) is hypothesized to be involved in the pathophysiological process of ischemia‑reperfusion injury, and exerts a protective effect on neurons. The aim of the present study was to investigate the effects of H2S on neural functions following cardiac arrest (CA) in rats. A total of 60 rats were allocated at random into three groups. CA was induced to establish the model and CPR was performed after 6 min. Subsequently, sodium hydrosulfide (NaHS), hydroxylamine or saline was administered to the rats. Serum levels of H2S, neuron‑specific enolase (NSE) and S100β were determined following CPR. In addition, neurological deficit scoring (NDS), the beam walking test (BWT), prehensile traction test and Morris water maze experiment were conducted. Neuronal apoptosis rates were detected in the hippocampal region following sacrifice. After CPR, as the H2S levels increased or decreased, the serum NSE and S100β concentrations decreased or increased, respectively (P<0.0w. The NDS results of the NaHS group were improved compared with those of the hydroxylamine group at 24 h after CPR (P<0.05). In the Morris water maze experiment, BWT and prehensile traction test the animals in the NaHS group performed best and rats in the hydroxylamine group performed worst. At day 7, the apoptotic index and the expression of caspase‑3 were reduced in the hippocampal CA1 region, while the expression of Bcl‑2 increased in the NaHS group; and results of the hydroxylamine group were in contrast. Therefore, the results of the present study indicate that H2S is able to improve neural function in rats following CPR.
View Figures
View References

Related Articles

Journal Cover

February-2016
Volume 11 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lin JY, Zhang MW, Wang JG, Li H, Wei HY, Liu R, Dai G and Liao XX: Hydrogen sulfide improves neural function in rats following cardiopulmonary resuscitation. Exp Ther Med 11: 577-587, 2016
APA
Lin, J., Zhang, M., Wang, J., Li, H., Wei, H., Liu, R. ... Liao, X. (2016). Hydrogen sulfide improves neural function in rats following cardiopulmonary resuscitation. Experimental and Therapeutic Medicine, 11, 577-587. https://doi.org/10.3892/etm.2015.2950
MLA
Lin, J., Zhang, M., Wang, J., Li, H., Wei, H., Liu, R., Dai, G., Liao, X."Hydrogen sulfide improves neural function in rats following cardiopulmonary resuscitation". Experimental and Therapeutic Medicine 11.2 (2016): 577-587.
Chicago
Lin, J., Zhang, M., Wang, J., Li, H., Wei, H., Liu, R., Dai, G., Liao, X."Hydrogen sulfide improves neural function in rats following cardiopulmonary resuscitation". Experimental and Therapeutic Medicine 11, no. 2 (2016): 577-587. https://doi.org/10.3892/etm.2015.2950