Effect of an EDA-A1 gene mutant on the proliferation and cell cycle distribution of cultured human umbilical vein endothelial cells

Lei,Ke; Wang,Lunchang; Ma,Bing; Shi,Ping; Li,Longjiang; Che,Tuanjie; He,Xiangyi

doi:10.3892/etm.2015.2952

February-2016 Volume 11 Issue 2

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February-2016 Volume 11 Issue 2

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Article

Effect of an EDA-A1 gene mutant on the proliferation and cell cycle distribution of cultured human umbilical vein endothelial cells

Authors:
- Ke Lei
- Lunchang Wang
- Bing Ma
- Ping Shi
- Longjiang Li
- Tuanjie Che
- Xiangyi He
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Affiliations: Department of Stomatology, Central Hospital of Guangyuan, Guangyuan, Sichuan 628000, P.R. China, Department of Respiratory Medicine, Central Hospital of Guangyuan, Guangyuan, Sichuan 628000, P.R. China, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Institute of Cell Biology, School of Life Science, Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Department of Prosthodontics, School of Stomatology, Lanzhou University, Lanzhou, Gansu 730000, P.R. China
Pages: 535-539
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Published online on: December 18, 2015

https://doi.org/10.3892/etm.2015.2952
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Abstract

Ectodysplasin (EDA) gene mutation is associated with hypohidrotic ectodermal dysplasia (HED). The aim of this study was to investigate the effect of ectodysplasin, transcript variant 1 (EDA‑A1) on the proliferation and cell cycle of ECV304 human umbilical vein endothelial cells (HUVECs). Recombinant eukaryotic expression vectors containing mutant (M) and wild‑type (W) EDA‑A1 coding sequences, pcDNA3.1 (‑)‑EDA‑A1‑M and pcDNA3.1 (‑)‑EDA‑A1‑W, respectively, were transfected into ECV304 cells. The EDA‑A1 gene was amplified by reverse transcription polymerase chain reaction (RT‑PCR), and the protein was detected by western blotting. The EDA‑A1 gene and protein were detected in ECV304 cells transfected with pcDNA3.1 (‑)‑EDA‑A1‑M and pcDNA3.1 (‑)‑EDA‑A1‑W, but not in ECV304 cells transfected with empty plasmid or cells that had not undergone transfection. Compared with the control group, the EDA‑A1 gene mutant significantly decreased the proliferation of ECV304 cells and its inhibition rate was 45.70% (P<0.01), whereas the wild‑type EDA‑A1 gene did not cause such growth inhibition (P>0.05). A significant increase of the fraction of cells in the G0/G1 phase of the cell cycle was observed in the ECV304 cells of the mutant group compared with wild type group, with an increase in the S phase population and a concomitant reduction in the G2/M phase population (P<0.05). These results indicate that compared with the wild‑type gene, transfection with a mutant EDA‑A1 gene inhibited the proliferation and cell cycle of cultured HUVECs.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of an EDA-A1 gene mutant on the proliferation and cell cycle distribution of cultured human umbilical vein endothelial cells

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