Open Access

Effect of the herbal formulation Jianpijiedu on the TCRVβCDR3 repertoire in rats with hepatocellular carcinoma and subjected to food restriction combined with laxative

  • Authors:
    • Baoguo Sun
    • Jun Meng
    • Ting Xiang
    • Lei Zhang
    • Liuxiang Deng
    • Yan Chen
    • Haoxuan Luo
    • Zhangbin Yang
    • Zexiong Chen
    • Shijun Zhang
  • View Affiliations

  • Published online on: December 23, 2015     https://doi.org/10.3892/etm.2015.2955
  • Pages: 818-826
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of this study was to investigate the effects of the Chinese herbal formulation Jianpijiedu (JPJD) in a rat model of orthotopic hepatocellular carcinoma (OHC). The tumor‑bearing rats underwent food restriction combined with laxative (FRL) treatment in order to model the nutritional and digestive symptoms of patients with hepatocellular carcinoma. In addition, the study aimed to elucidate the effect of JPJD on the T cell receptor Vβ-chain complementarity-determining region 3 (TCRVβCDR3) repertoire and the underlying mechanism. The FRL rat model was established by alternate‑day food restriction and the oral administration of Glauber’s salt (sodium sulfate), based on which the OHC model was then established. Subsequently, the FRL‑OHC induced animals received JPJD or thymopentin‑5 (TP5) for 17 days. Differences in the TCRVβCDR3 repertoire in the rat thymus, liver and hepatocellular carcinoma tissues were analyzed by polymerase chain reaction. Compared with the FRL‑OHC model animals without any treatment, those treated with JPJD exhibited significantly inhibited hepatocellular carcinoma growth (P<0.05), reduced weight loss (P<0.01) and stable visceral indices (P<0.05). Furthermore, the JPJD treatment appeared to improve Simpson's diversity index (Ds) values and the quasi‑Gaussian distribution rate of the TCRVβCDR3 repertoire in the thymus, liver and hepatocellular carcinoma tissues. However, no anti‑hepatoma effects were evident in the rats treated with TP5. In addition, TP5 increased the Ds values and the quasi‑Gaussian distribution rate of the TCRVβCDR3 repertoire in hepatocellular carcinoma tissues compared with those in the JPJD‑treated group. The anti‑hepatoma effects of JPJD in FRL‑OHC‑induced animals may be due to the promotion of the Ds values of the TCRVβCDR3 repertoire.
View Figures
View References

Related Articles

Journal Cover

March-2016
Volume 11 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Sun B, Meng J, Xiang T, Zhang L, Deng L, Chen Y, Luo H, Yang Z, Chen Z, Zhang S, Zhang S, et al: Effect of the herbal formulation Jianpijiedu on the TCRVβCDR3 repertoire in rats with hepatocellular carcinoma and subjected to food restriction combined with laxative. Exp Ther Med 11: 818-826, 2016
APA
Sun, B., Meng, J., Xiang, T., Zhang, L., Deng, L., Chen, Y. ... Zhang, S. (2016). Effect of the herbal formulation Jianpijiedu on the TCRVβCDR3 repertoire in rats with hepatocellular carcinoma and subjected to food restriction combined with laxative. Experimental and Therapeutic Medicine, 11, 818-826. https://doi.org/10.3892/etm.2015.2955
MLA
Sun, B., Meng, J., Xiang, T., Zhang, L., Deng, L., Chen, Y., Luo, H., Yang, Z., Chen, Z., Zhang, S."Effect of the herbal formulation Jianpijiedu on the TCRVβCDR3 repertoire in rats with hepatocellular carcinoma and subjected to food restriction combined with laxative". Experimental and Therapeutic Medicine 11.3 (2016): 818-826.
Chicago
Sun, B., Meng, J., Xiang, T., Zhang, L., Deng, L., Chen, Y., Luo, H., Yang, Z., Chen, Z., Zhang, S."Effect of the herbal formulation Jianpijiedu on the TCRVβCDR3 repertoire in rats with hepatocellular carcinoma and subjected to food restriction combined with laxative". Experimental and Therapeutic Medicine 11, no. 3 (2016): 818-826. https://doi.org/10.3892/etm.2015.2955