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Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration

  • Authors:
    • Zihao Feng
    • Jiaqi Liu
    • Congcong Shen
    • Nanhang Lu
    • Yong Zhang
    • Yanwen Yang
    • Fazhi Qi
  • View Affiliations / Copyright

    Affiliations: Department of Plastic and Reconstructive Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 737-746
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    Published online on: December 29, 2015
       https://doi.org/10.3892/etm.2015.2961
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Abstract

The present study aimed to investigate the properties of a promising bone scaffold for bone repair, which consisted of a novel composite of adipose‑derived stem cells (ADSCs) attached to a porous β‑tricalcium phosphate (β‑TCP) scaffold with platelet‑rich plasma (PRP). The β‑TCP powder was synthesized and its composition was determined using X‑ray diffraction and Fourier transform infrared spectroscopy. The surface morphology and microstructure of the fabricated porous β‑TCP scaffold samples were analyzed using light and scanning electron microscopy, and their porosity and compressive strength were also evaluated. In addition, the viability of rabbit ADSCs incubated with various concentrations of the β‑TCP extraction fluid was analyzed. The rate of attachment and the morphology of biotinylated ADSCs (Bio‑ADSCs) on avidin‑coated β‑TCP (Avi‑β‑TCP), and untreated ADSCs on β‑TCP, were compared. Furthermore, in vivo bone‑forming abilities were determined following the implantation of group 1 (Bio‑ADSCs/Avi‑β‑TCP) and group 2 (Bio‑ADSCs/Avi‑β‑TCP/PRP) constructs using computed tomography, and histological osteocalcin (OCN) and alkaline phosphatase (ALP) expression analyses in a rabbit model of mandibulofacial defects. The β‑TCP scaffold exhibited a high porosity (71.26±0.28%), suitable pore size, and good mechanical strength (7.93±0.06 MPa). Following incubation with β‑TCP for 72 h, 100% of viable ADSCs remained. The avidin‑biotin binding system significantly increased the initial attachment rate of Bio‑ADSCs to Avi‑β‑TCP in the first hour (P<0.01). Following the addition of PRP, group 2 exhibited a bony‑union and mandibular body shape, newly formed bone and increased expression levels of OCN and ALP in the mandibulofacial defect area, as compared with group 1 (P<0.05). The results of the present study suggested that the novel Bio‑ADSCs/Avi‑β‑TCP/PRP composite may have potential application in bone repair and bone tissue engineering.
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Copy and paste a formatted citation
Spandidos Publications style
Feng Z, Liu J, Shen C, Lu N, Zhang Y, Yang Y and Qi F: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration. Exp Ther Med 11: 737-746, 2016.
APA
Feng, Z., Liu, J., Shen, C., Lu, N., Zhang, Y., Yang, Y., & Qi, F. (2016). Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration. Experimental and Therapeutic Medicine, 11, 737-746. https://doi.org/10.3892/etm.2015.2961
MLA
Feng, Z., Liu, J., Shen, C., Lu, N., Zhang, Y., Yang, Y., Qi, F."Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration". Experimental and Therapeutic Medicine 11.3 (2016): 737-746.
Chicago
Feng, Z., Liu, J., Shen, C., Lu, N., Zhang, Y., Yang, Y., Qi, F."Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration". Experimental and Therapeutic Medicine 11, no. 3 (2016): 737-746. https://doi.org/10.3892/etm.2015.2961
Copy and paste a formatted citation
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Spandidos Publications style
Feng Z, Liu J, Shen C, Lu N, Zhang Y, Yang Y and Qi F: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration. Exp Ther Med 11: 737-746, 2016.
APA
Feng, Z., Liu, J., Shen, C., Lu, N., Zhang, Y., Yang, Y., & Qi, F. (2016). Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration. Experimental and Therapeutic Medicine, 11, 737-746. https://doi.org/10.3892/etm.2015.2961
MLA
Feng, Z., Liu, J., Shen, C., Lu, N., Zhang, Y., Yang, Y., Qi, F."Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration". Experimental and Therapeutic Medicine 11.3 (2016): 737-746.
Chicago
Feng, Z., Liu, J., Shen, C., Lu, N., Zhang, Y., Yang, Y., Qi, F."Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration". Experimental and Therapeutic Medicine 11, no. 3 (2016): 737-746. https://doi.org/10.3892/etm.2015.2961
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