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Article

Curcumin suppresses transforming growth factor-β1-induced cardiac fibroblast differentiation via inhibition of Smad-2 and p38 MAPK signaling pathways

  • Authors:
    • Huzi Liu
    • Aijun Liu
    • Chunli Shi
    • Bao Li
  • View Affiliations / Copyright

    Affiliations: Department of Cardiac Surgery, Shanxi Cardiovascular Hospital, Taiyuan, Shanxi 030024, P.R. China, Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China, Outpatient Department, Shanxi Cardiovascular Hospital, Taiyuan, Shanxi 030024, P.R. China, Department of Cardiology, Shanxi Cardiovascular Hospital, Taiyuan, Shanxi 030024, P.R. China
  • Pages: 998-1004
    |
    Published online on: January 8, 2016
       https://doi.org/10.3892/etm.2016.2969
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Abstract

The differentiation of cardiac fibroblasts (CFs) into myofibroblasts and the subsequent deposition of the extracellular matrix is associated with myocardial fibrosis following various types of myocardial injury. In the present study, the effect of curcumin, which is a pharmacologically‑safe natural compound from the Curcuma longa herb, on transforming growth factor (TGF)‑β1‑induced CFs was investigated, and the underlying molecular mechanisms were examined. The expression levels of α‑smooth muscle actin (SMA) stress fibers were investigated using western blotting and immunofluorescence in cultured neonatal rat CFs. Protein and mRNA expression levels of α‑SMA and collagen type I (ColI) were determined by western blotting and reverse transcription‑quantitative polymerase chain reaction. In addition, the activation of Smad2 and p38 was examined using western blotting. Curcumin, SB431542 (a TGF‑βR‑Smad2 inhibitor) and SB203580 (a p38 inhibitor) were used to inhibit the stimulation by TGF‑β1. The results demonstrated that the TGF‑β1‑induced expression of α‑SMA and ColI was suppressed by curcumin at the mRNA and protein levels, while SB431542 and SB203580 induced similar effects. Furthermore, phosphorylated Smad‑2 and p38 were upregulated in TGF‑β1‑induced CFs, and these effects were substantially inhibited by curcumin administration. In conclusion, the results of the present study demonstrated that treatment with curcumin effectively suppresses TGF‑β1‑induced CF differentiation via Smad‑2 and p38 signaling pathways. Thus, curcumin may be a potential therapeutic agent for the treatment of cardiac fibrosis.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Liu A, Shi C and Li B: Curcumin suppresses transforming growth factor-β1-induced cardiac fibroblast differentiation via inhibition of Smad-2 and p38 MAPK signaling pathways. Exp Ther Med 11: 998-1004, 2016.
APA
Liu, H., Liu, A., Shi, C., & Li, B. (2016). Curcumin suppresses transforming growth factor-β1-induced cardiac fibroblast differentiation via inhibition of Smad-2 and p38 MAPK signaling pathways. Experimental and Therapeutic Medicine, 11, 998-1004. https://doi.org/10.3892/etm.2016.2969
MLA
Liu, H., Liu, A., Shi, C., Li, B."Curcumin suppresses transforming growth factor-β1-induced cardiac fibroblast differentiation via inhibition of Smad-2 and p38 MAPK signaling pathways". Experimental and Therapeutic Medicine 11.3 (2016): 998-1004.
Chicago
Liu, H., Liu, A., Shi, C., Li, B."Curcumin suppresses transforming growth factor-β1-induced cardiac fibroblast differentiation via inhibition of Smad-2 and p38 MAPK signaling pathways". Experimental and Therapeutic Medicine 11, no. 3 (2016): 998-1004. https://doi.org/10.3892/etm.2016.2969
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Liu A, Shi C and Li B: Curcumin suppresses transforming growth factor-β1-induced cardiac fibroblast differentiation via inhibition of Smad-2 and p38 MAPK signaling pathways. Exp Ther Med 11: 998-1004, 2016.
APA
Liu, H., Liu, A., Shi, C., & Li, B. (2016). Curcumin suppresses transforming growth factor-β1-induced cardiac fibroblast differentiation via inhibition of Smad-2 and p38 MAPK signaling pathways. Experimental and Therapeutic Medicine, 11, 998-1004. https://doi.org/10.3892/etm.2016.2969
MLA
Liu, H., Liu, A., Shi, C., Li, B."Curcumin suppresses transforming growth factor-β1-induced cardiac fibroblast differentiation via inhibition of Smad-2 and p38 MAPK signaling pathways". Experimental and Therapeutic Medicine 11.3 (2016): 998-1004.
Chicago
Liu, H., Liu, A., Shi, C., Li, B."Curcumin suppresses transforming growth factor-β1-induced cardiac fibroblast differentiation via inhibition of Smad-2 and p38 MAPK signaling pathways". Experimental and Therapeutic Medicine 11, no. 3 (2016): 998-1004. https://doi.org/10.3892/etm.2016.2969
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