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Article

CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice

  • Authors:
    • Jian‑Guo Li
    • Yu‑Mo Du
    • Zhi‑Dong Yan
    • Jia Yan
    • Yong‑Xun Zhuansun
    • Rui Chen
    • Wei Zhang
    • Su‑Ling Feng
    • Pi‑Xin Ran
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Sun Yat‑Sen Memorial Hospital, Institute of Respiratory Diseases, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China, Department of Intensive Care Unit, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Department of Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong 510120, P.R. China
  • Pages: 878-884
    |
    Published online on: January 13, 2016
       https://doi.org/10.3892/etm.2016.2989
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Abstract

Dendritic cells (DCs) are associated with the activation and differentiation of T helper (Th) cells. Cluster of differentiation (CD)80 and CD86, the co‑stimulatory molecules highly expressed in DCs, have are prominent in promoting the differentiation of Th cells toward Th2 cells. However, little is known about the effect of CD80 and CD86 knockdown on Th1/Th2 cytokine production in mature DCs (mDCs). The aim of the present study was to investigate whether small‑interfering RNA (siRNA) could suppress the surface expression of CD80 and CD86 in mDCs. The effects of CD80 and CD86 knockdown in mDCs on Th1/Th2 cytokine expression were examined using an asthmatic murine model. DCs were isolated, separated and cultured in vitro. Flow cytometry was used to examine the expression of CD11c, CD80 and CD86 on the DCs. The DCs were transfected with CD80‑ and CD86‑specific siRNA, while non‑siRNA and negative siRNA controls were also designed. Then, the mRNA and protein expression levels of CD80 and CD86 were determined by reverse transcription-quantitative polymerase chain reaction and flow cytometry, respectively. The levels of interferon (IFN)‑γ and interleukin (IL)‑4 produced by T cells co‑cultured with mDCs were measured by enzyme‑linked immunosorbent assay. Substantial downregulation of CD80 and CD86 mRNA and protein levels were observed in the mDCs following transfection with siRNA. The level of IFN‑γ produced by T cells co‑cultured with mDCs was significantly increased in the siRNA group, while IL‑4 production was significantly decreased. These results show that specific targeting of CD80 and CD86 with siRNA is able to suppress CD80/CD86 expression and consequently regulate Th1/Th2 cytokine levels by increasing IFN‑γ production and decreasing IL‑4 levels in an asthmatic murine model.
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1 

Locksley RM: Asthma and allergic inflammation. Cell. 140:777–783. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Holgate ST: Innate and adaptive immune responses in asthma. Nat Med. 18:673–683. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Larché M, Robinson DS and Kay AB: The role of T lymphocytes in the pathogenesis of asthma. J Allergy Clin Immunol. 111:450–464. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Lambrecht BN and Hammad H: The role of dendritic and epithelial cells as master regulators of allergic airway inflammation. Lancet. 376:835–843. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Holt PG and Upham JW: The role of dendritic cells in asthma. Curr Opin Allergy Clin Immunol. 4:39–44. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Lim TS, Goh JK, Mortellaro A, Lim CT, Hämmerling GJ and Ricciardi-Castagnoli P: CD80 and CD86 differentially regulate mechanical interactions of T-cells with antigen-presenting dendritic cells and B-cells. PLoS One. 7:e451852012. View Article : Google Scholar : PubMed/NCBI

7 

Wong CK, Lun SW, Ko FW, Ip WK, Hui DS and Lam CW: Increased expression of plasma and cell surface co-stimulatory molecules CTLA-4, CD28 and CD86 in adult patients with allergic asthma. Clin Exp Immunol. 141:122–129. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Shi HZ, Xie ZF, Deng JM, Chen YQ and Xiao CQ: Soluble CD86 protein in serum samples of patients with asthma. Thorax. 59:870–875. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Van Rijt LS and Lambrecht BN: Dendritic cells in asthma: a function beyond sensitization. Clin Exp Allergy. 35:1125–34. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Van Rijt LS, Vos N, Willart M, Kleinjan A, Coyle AJ, Hoogsteden HC and Lambrecht BN: Essential role of dendritic cell CD80/CD86 costimulation in the induction, but not reactivation, of TH2 effector responses in a mouse model of asthma. J Allergy Clin Immunol. 114:166–173. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Wu SG, Wang GL, Li LY and Ji J: Effects of microRNA-21 on the interleukin 12/signal transducer and activator of transcription 4 signaling pathway in asthmatic mice. Cent Eur J Immunol. 39:40–45. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Gu X, Xiang J, Yao Y and Chen Z: Effects of RNA interference on CD80 and CD86 expression in bone marrow-derived murine dendritic cells. Scand J Immunol. 64:588–594. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−∆∆Ct method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Fanta CH: Asthma. N Engl J Med. 360:1002–1014. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Lambrecht BN and Hammad H: Lung dendritic cells in respiratory viral infection and asthma: From protection to immunopathology. Annu Rev Immunol. 30:243–270. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Wenzel SE: Asthma phenotypes: The evolution from clinical to molecular approaches. Nat Med. 18:716–725. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Hansel TT, Johnston SL and Openshaw PJ: Microbes and mucosal immune responses in asthma. Lancet. 381:861–873. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Compalati E, Braido F and Canonica GW: An update on allergen immunotherapy and asthma. Curr Opin Pulm Med. 20:109–117. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Goyvaerts C, Dingemans J, De Groeve K, Heirman C, Van Gulck E, Vanham G, De Baetselier P, Thielemans K, Raes G and Breckpot K: Targeting of human antigen-presenting cell subsets. J Virol. 87:11304–11308. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Reise Sousa C: Dendritic cells in a mature age. Nat Rev Immunol. 6:476–483. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Gill MA: The role of dendritic cells in asthma. J Allergy Clin Immunol. 129:889–901. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Shi JH, LI YG and LI TS: The roles of dendritic cells in antigen presentation and the pathogenesis of asthma. Zhonghua Jie He He Hu Xi Za Zhi. 28:22–27. 2005.(In Chinese). PubMed/NCBI

23 

Wong CK, Lun SW, Ko FW, Ip WK, Hui DS and Lam CW: Increased expression of plasma and cell surface co-stimulatory molecules CTLA-4, CD28 and CD86 in adult patients with allergic asthma. Clin Exp Immunol. 141:122–129. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Bellou A and Finn PW: Costimulation: Critical pathways in the immunologic regulation of asthma. Curr Allergy Asthma Rep. 5:149–154. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Chen YQ and Shi HZ: CD28/CTLA-4-CD80/CD86 and ICOS-B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61:15–26. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Bieber T, Novak N, Herrmann N and Koch S: Role of dendritic cells in atopic dermatitis: An update. Clin Rev Allergy Immunol. 41:254–258. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Hespel C and Moser M: Role of inflammatory dendritic cells in innate and adaptive immunity. Eur J Immunol. 42:2535–2543. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Novak N: An update on the role of human dendritic cells in patients with atopic dermatitis. J Allergy Clin Immunol. 129:879–886. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Lombardi V, Singh AK and Akbari O: The role of costimulatory molecules in allergic disease and asthma. Int Arch Allergy Immunol. 151:179–189. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Zhang Y, Zhou X and Zhou B: DC-derived TSLP promotes Th2 polarization in LPS-primed allergic airway inflammation. Eur J Immunol. 42:1735–1743. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Crosby JR, Guha M, Tung D, Miller DA, Bender B, Condon TP, York-DeFalco C, Geary RS, Monia BP, Karras JG and Gregory SA: Inhaled CD86 antisense oligonucleotide suppresses pulmonary inflammation and airway hyper-responsiveness in allergic mice. J Pharmacol Exp Ther. 321:938–946. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE and Mello CC: Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 391:806–811. 1998. View Article : Google Scholar : PubMed/NCBI

33 

Ghafouri-Fard S: siRNA and cancer immunotherapy. Immunotherapy. 4:907–917. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Gavrilov K and Saltzman WM: Therapeutic siRNA: Principles, challenges and strategies. Yale J Biol Med. 85:187–200. 2012.PubMed/NCBI

35 

Yan WJ, Xu SJ, Xie MM and Wu BL: Effects of exogenous cyclic dimeric guanosinemonophosphate on the gene expression of Streptococcus mutans. Zhongguo Zu Zhi Gong Cheng Yan Jiu. 16:1451–1454. 2012.(In Chinese).

36 

Lee CC, Huang HY and Chiang BL: Lentiviral-mediated interleukin-4 and interleukin-13 RNA interference decrease airway inflammation and hyperresponsiveness. Hum Gene Ther. 22:577–686. 2011. View Article : Google Scholar : PubMed/NCBI

37 

Li Y, Sun M, Cheng H, Li S, Liu L, Qiao H, Hua S and Lu J: Silencing IL-23 expression by a small hairpin RNA protects against asthma in mice. Exp Mol Med. 43:197–204. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Woska JJ and Gillespie ME: Small-interfering RNA-mediated identification and regulation of the ternary SNARE complex mediating RBL-2H3 mast cell degranulation. Scand J Immunol. 73:8–17. 2011. View Article : Google Scholar : PubMed/NCBI

39 

Lu XX, McCoy KS, Xu JL, Hu WK and Chen HB: Small interfering RNA targeting T-cell Ig mucin-3 decreases allergic airway inflammation and hyperresponsiveness. Inflammation. 36:582–591. 2013. View Article : Google Scholar : PubMed/NCBI

40 

Darcan-Nicolaisen Y, Meinicke H, Fels G, Hegend O, Haberland A, Kühl A, Loddenkemper C, Witzenrath M, Kube S, Henke W and Hamelmann E: Small interfering RNA against transcription factor STAT6 inhibits allergic airway inflammation and hyperreactivity in mice. J Immunol. 182:7501–7508. 2009. View Article : Google Scholar : PubMed/NCBI

41 

Moriwaki A, Inoue H, Nakano T, Matsunaga Y, Matsuno Y, Matsumoto T, Fukuyama S, Kan-O K, Matsumoto K, Tsuda-Eguchi M, et al: T cell treatment with small interfering RNA for suppressor of cytokine signaling 3 modulates allergic airway responses in a murine model of asthma. Am J Respir Cell Mol Biol. 44:448–455. 2011. View Article : Google Scholar : PubMed/NCBI

42 

Zheng YH, Lu JJ, Guo ZL, Ren T and Liang YJ: Small interfering RNAs specific for spleen tyrosine kinase inhibit maturation of dendritic cells of asthmatic mice in vitro. Zhongguo Hu Xi Yu Wei Zhong Jian Hu Za Zhi. 8:487–491. 2009.(In Chinese).

43 

Li JG, Zhuansun YX, Ran PX, Zhang W, Mo XN, Wu H and Li YQ: Effects of bone marrow mesenchymal stem cells on CD4+CD25+ regulatory T cells and airway inflammation in asthmatic mice. Zhongguo Zu Zhi Gong Cheng Yan Jiu. 12:9302–9305. 2008.(In Chinese).

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Copy and paste a formatted citation
Spandidos Publications style
Li JG, Du YM, Yan ZD, Yan J, Zhuansun YX, Chen R, Zhang W, Feng SL and Ran PX: CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice. Exp Ther Med 11: 878-884, 2016.
APA
Li, J., Du, Y., Yan, Z., Yan, J., Zhuansun, Y., Chen, R. ... Ran, P. (2016). CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice. Experimental and Therapeutic Medicine, 11, 878-884. https://doi.org/10.3892/etm.2016.2989
MLA
Li, J., Du, Y., Yan, Z., Yan, J., Zhuansun, Y., Chen, R., Zhang, W., Feng, S., Ran, P."CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice". Experimental and Therapeutic Medicine 11.3 (2016): 878-884.
Chicago
Li, J., Du, Y., Yan, Z., Yan, J., Zhuansun, Y., Chen, R., Zhang, W., Feng, S., Ran, P."CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice". Experimental and Therapeutic Medicine 11, no. 3 (2016): 878-884. https://doi.org/10.3892/etm.2016.2989
Copy and paste a formatted citation
x
Spandidos Publications style
Li JG, Du YM, Yan ZD, Yan J, Zhuansun YX, Chen R, Zhang W, Feng SL and Ran PX: CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice. Exp Ther Med 11: 878-884, 2016.
APA
Li, J., Du, Y., Yan, Z., Yan, J., Zhuansun, Y., Chen, R. ... Ran, P. (2016). CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice. Experimental and Therapeutic Medicine, 11, 878-884. https://doi.org/10.3892/etm.2016.2989
MLA
Li, J., Du, Y., Yan, Z., Yan, J., Zhuansun, Y., Chen, R., Zhang, W., Feng, S., Ran, P."CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice". Experimental and Therapeutic Medicine 11.3 (2016): 878-884.
Chicago
Li, J., Du, Y., Yan, Z., Yan, J., Zhuansun, Y., Chen, R., Zhang, W., Feng, S., Ran, P."CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice". Experimental and Therapeutic Medicine 11, no. 3 (2016): 878-884. https://doi.org/10.3892/etm.2016.2989
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