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Article

Effects of lactadherin on plasma D-lactic acid and small intestinal MUC2 and claudin-1 expression levels in rats with rotavirus-induced diarrhea

  • Authors:
    • Rui Xu
    • Yi‑Hui Lei
    • Jun Shi
    • Yi‑Jun Zhou
    • Ying‑Wei Chen
    • Zhen‑Juan He
  • View Affiliations / Copyright

    Affiliations: Department of Neonatology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R. China, Department of Perinatal Research Laboratory, Shanghai Institute for Pediatric Research Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R. China
  • Pages: 943-950
    |
    Published online on: January 22, 2016
       https://doi.org/10.3892/etm.2016.3015
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Abstract

The aim of the present study was to investigate the effects of lactadherin on plasma D‑lactic acid and small intestinal mucin (MUC) 2 and claudin‑1 expression levels in rats with diarrhea induced by rotavirus (RV) infection. A total of 75 seven‑day‑old healthy Sprague‑Dawley rats were randomly divided into the following five groups: Control (C), RV infection (RVI), lactadherin before rotavirus infection (LBRI), lactadherin after rotavirus infection (LARI), and blank (B). On day 4 of artificial feeding, the rats in groups RVI, LBRI and LARI were intragastric administered 1x106 PFU RV; whereas the rats in groups C and B were intragastrically administered an equal volume of maintenance solution from the RV supernatant and normal saline, respectively. In the LBRI and LARI groups, rats received daily intragastric administration of 0.25 mg lactadherin for three days prior to and following infection with RV, respectively. The course of diarrheal symptoms was observed in each group and samples were collected on days 1, 4, and 7 post‑infection in order to determine the mucosal morphology, plasma D‑lactic acid levels and the expression levels of MUC2 and the intracellular junction protein, claudin-1, in the small intestine. On day 4 post‑infection, the rats in group RVI demonstrated severely damaged small intestines and typical diarrheal characteristics, as detected by light microscopy; whereas rats in groups LBRI and LARI demonstrated intact small intestinal villi with partial vacuolation of epithelial cells and changes in the position of their nuclei. Electron microscopy demonstrated that the rats in the RVI group had sparse, shortened, disordered intestinal microvilli and widened intercellular junctions; whereas those in groups LBRI and LARI had long intestinal microvilli sparser compared with groups B and C and slightly widened intercellular junctions. Plasma D‑lactic acid levels were increased in groups RVI, LBRI and LARI, as compared with groups B and C, and the greatest levels were detected in the RVI group on days 1, 4 and 7 post‑infection. In addition to maintaining intestinal permeability, lactadherin enhanced the expression levels of MUC2 and reduced the expression of claudin‑1; therefore, further protecting the intestinal epithelial barrier, which may contribute to the prevention and treatment of diarrhea induced by infection with RV.
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1 

Walker CL, Rudan I, Liu L, Nair H, Theodoratou E, Bhutta ZA, O'Brien KL, Campbell H and Black RE: Global burden of childhood pneumonia and diarrhoea. Lancet. 381:1405–1416. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Lanata CF, Fischer-Walker CL, Olascoaga AC, Torres CX, Aryee MJ and Black RE: Child Health Epidemiology Reference Group of the World Health Organization and, UNICEF. Global causes of diarrheal disease mortality in children <5 years of age: A systematic review. PLoS one. 8:e727882013. View Article : Google Scholar : PubMed/NCBI

3 

Chen SC, Tan LB, Huang LM and Chen KT: Rotavirus infection and the current status of rotavirus vaccines. J Formos Med Assoc. 111:183–193. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Kvistgaard AS, Pallesen LT, Arias CF, López S, Petersen TE, Heegaard CW and Rasmussen JT: Inhibitory effects of human and bovine milk constituents on rotavirus infections. J Dairy Sci. 87:4088–4096. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Bu HF, Zuo XL, Wang X, Ensslin MA, Koti V, Hsueh W, Raymond AS, Shur BD and Tan XD: Milk fat globule-EGF factor 8/lactadherin plays a curcial role in maintenance and repair of murine intestinal epithelium. J Clin invest. 117:3673–3683. 2007.PubMed/NCBI

6 

Kusunoki R, Ishihara S, Aziz M, Oka A, Tada Y and Kinoshita Y: Role of milk fat globule-epidermal growth factor 8 in intestinal inflammation. Digestion. 85:103–107. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Ciarlet M and Estes MK: Interactions between rotavirus and gastrointestinal cells. Curr Opin Microbiol. 4:435–441. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Kanno T, Koyanagi N, Katoku Y, Yonekubo A, Yajima T, Kuwata T, Kitagawa H and Harada E: Simplified preparation of a refined milk formula comparable to rat's milk: Influence of the formula on development of the gut and brain in artificially reared rat pups. J Pediatr Gastroenterol Nutr. 24:242–252. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Lu H, Zhu J, Zang Y, Ze Y and Qin J: Cloning, high level expression of human paraoxonase-3 in Sf9 cells and pharmacological characterization of its product. Biochem Pharmacol. 70:1019–1025. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Kaito MI, Ishida S, Tanaka H, Horiike S, Fujita N, Adachi Y, Kohara M, Konishi M and Watanabe S: Morphology of hepatitis C and hepatitis B virus particles as detected by immunogold electron microscopy. Med Mol Morph. 39:63–71. 2006. View Article : Google Scholar

11 

Boshuizen JA, Reimerink JH, van Korteland Male AM, van Ham VJ, Koopmans MP, Büller HA, Dekker J and Einerhand AW: Changes in small intestinal homeostasis, morphology and gene expression during rotavirus infection of infant mice. J Virol. 77:13005–13016. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Li JY, Lu Y, Hu S, Sun D and Yao YM: Preventive effect of glutamine on intestinal barrier dysfunction induced by severe trauma. World J Gastroenterol. 8:168–171. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Yeung T, Gilbert GE, Shi J, Silvius J, Kapus A and Grinstein S: Membrane phosphatidylserine regulates surface charge and protein localization. Science. 319:210–213. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Yang HM, Gao J, Sheng HY, Zhou Y, Chen TX, Zhu JX and He ZJ: Effect of lactadherin on IL-2, IL-4 and INF-γ secreted by intestinal tract of newborn rats with rotavirus infection. Guo Ji Er Ke Xue Za Zhi. 36:328–330. 2009.(In Chinese).

15 

Yang LY, He ZJ and Zhu JX: The effect of lactadherin in human milk on protecting diarrhea induced by rotavirus infection. Guo Ji Er Ke Xue Za Zhi. 32:174–176. 2005.(In Chinese).

16 

Zhou YJ, Gao J, Yang HM, Yuan XL, Chen TX and He ZJ: The role of the lactadherin in promoting intestinal DCs development in vivo and vitro. Clin Dev Immunol. 2010:3575412010. View Article : Google Scholar : PubMed/NCBI

17 

Dong HT: Research of lactadherin's function. Guo Ji Er Ke Xue Za Zhi. 37:515–517. 2010.(In Chinese).

18 

Li WD, Jia L, Ou Y, Huang YX and Jiang SM: Surveillance of intra-abdominal pressure and intestinal barrier function in a rat model of acute necrotizing pancreatitis and its potential early therapeutic window. PLoS One. 8:e789752013. View Article : Google Scholar : PubMed/NCBI

19 

Shimizu K, Ogura H, Goto M, Asahara T, Nomoto K, Morotomi M, Yoshiya K, Matsushima A, Sumi Y, Kuwagata Y, et al: Altered gut flora and environment in patients with severe SIRS. J Trauma. 60:126–133. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Vella A and Farrugia G: D-lactate acidosis: Pathologic consequence of saprophytism. Mayo Clin Proc. 73:451–456. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Moghaddam HS, Moghaddam HN, Kermanshahi H, Houssavi AH and Raji A: The effect of vitamin A on Mucin2 gene expression, histological and performance of broiler chicken. Global Veterinaria. 5:168–174. 2010.

22 

Boshuizen JA, Reimerink JH, van Korteland Male AM, van Ham VJ, Bouma J, Gerwig GJ, Koopmans MP, Büller HA, Dekker J and Einerhand AW: Homeostasis and function of goblet cells during rotavirus infection in mice. Virology. 337:210–221. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Deplancke B and Gaskins HR: Microbial modulation of innate defense: goblet cells and the intestinal mucus layer. Am J Clin Nutr. 73:1131S–1141S. 2001.PubMed/NCBI

24 

Faure M, Moënnoz D, Montigon F, Mettraux C, Mercier S, Schiffrin EJ, Obled C, Breuillé D and Boza J: Mucin production and composition is altered in dextran sulfate sodium-induced colitis in rats. Dig Dis Sci. 48:1366–1373. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Sharma R, Young C and Neu J: Molecular modulation of intestinal epithelial barrier: Contribution of microbiota. J Biomed Biotechnol. 2010:3058792010. View Article : Google Scholar : PubMed/NCBI

26 

Groschwitz KR and Hogan SP: Intestinal barrier function: Molecular regulation and disease pathogenesis. J Allergy Clin Immunol. 124:3–20. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Anthony T Blikslager: Mucosal epithelial barrier repair to maintain pig health. Livestock Science. 133:194–199. 2010. View Article : Google Scholar

28 

Zhou L: Progress in the pathogenesis of rotavirus-associated disease. Guo Ji Er Ke Xue Za Zhi. 37:232–234. 2010.(In Chinese).

29 

Colbère-Garapin F, Martin-Latil S, Blondel B, Mousson L, Pelletier I, Autret A, François A, Niborski V, Grompone G, Catonnet G and van de Moer A: Prevention and treatment of enteric viral infections: Possible benefits of probiotic bacteria. Microbes Infect. 9:1623–1631. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Ehehalt R, Braun A, Karner M, Füllekrug J and Stremmel W: Phosphatidylcholine as a constituent in the colonic mucosal barrier: Physiological and clinical relevance. Biochim Biophys Acta. 1801:983–993. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Irvine EJ and Marshall JK: Increased intestinal permeability precedes the onset of Crohn's disease in a subject with familial risk. Gastroenterology. 119:1740–1744. 2000. View Article : Google Scholar : PubMed/NCBI

32 

Poritz LS, Harris LR III, Kelly AA and Koltun WA: Increase in the tight junction protein claudin-1 in intestinal inflammation. Dig Dis Sci. 56:2802–2809. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu R, Lei YH, Shi J, Zhou YJ, Chen YW and He ZJ: Effects of lactadherin on plasma D-lactic acid and small intestinal MUC2 and claudin-1 expression levels in rats with rotavirus-induced diarrhea. Exp Ther Med 11: 943-950, 2016.
APA
Xu, R., Lei, Y., Shi, J., Zhou, Y., Chen, Y., & He, Z. (2016). Effects of lactadherin on plasma D-lactic acid and small intestinal MUC2 and claudin-1 expression levels in rats with rotavirus-induced diarrhea. Experimental and Therapeutic Medicine, 11, 943-950. https://doi.org/10.3892/etm.2016.3015
MLA
Xu, R., Lei, Y., Shi, J., Zhou, Y., Chen, Y., He, Z."Effects of lactadherin on plasma D-lactic acid and small intestinal MUC2 and claudin-1 expression levels in rats with rotavirus-induced diarrhea". Experimental and Therapeutic Medicine 11.3 (2016): 943-950.
Chicago
Xu, R., Lei, Y., Shi, J., Zhou, Y., Chen, Y., He, Z."Effects of lactadherin on plasma D-lactic acid and small intestinal MUC2 and claudin-1 expression levels in rats with rotavirus-induced diarrhea". Experimental and Therapeutic Medicine 11, no. 3 (2016): 943-950. https://doi.org/10.3892/etm.2016.3015
Copy and paste a formatted citation
x
Spandidos Publications style
Xu R, Lei YH, Shi J, Zhou YJ, Chen YW and He ZJ: Effects of lactadherin on plasma D-lactic acid and small intestinal MUC2 and claudin-1 expression levels in rats with rotavirus-induced diarrhea. Exp Ther Med 11: 943-950, 2016.
APA
Xu, R., Lei, Y., Shi, J., Zhou, Y., Chen, Y., & He, Z. (2016). Effects of lactadherin on plasma D-lactic acid and small intestinal MUC2 and claudin-1 expression levels in rats with rotavirus-induced diarrhea. Experimental and Therapeutic Medicine, 11, 943-950. https://doi.org/10.3892/etm.2016.3015
MLA
Xu, R., Lei, Y., Shi, J., Zhou, Y., Chen, Y., He, Z."Effects of lactadherin on plasma D-lactic acid and small intestinal MUC2 and claudin-1 expression levels in rats with rotavirus-induced diarrhea". Experimental and Therapeutic Medicine 11.3 (2016): 943-950.
Chicago
Xu, R., Lei, Y., Shi, J., Zhou, Y., Chen, Y., He, Z."Effects of lactadherin on plasma D-lactic acid and small intestinal MUC2 and claudin-1 expression levels in rats with rotavirus-induced diarrhea". Experimental and Therapeutic Medicine 11, no. 3 (2016): 943-950. https://doi.org/10.3892/etm.2016.3015
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