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Article

Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling

  • Authors:
    • Qifa Ye
    • Yi Zhu
    • Shaojun Ye
    • Hong Liu
    • Xingguo She
    • Ying Niu
    • Yingzi Ming
  • View Affiliations / Copyright

    Affiliations: Center of Transplant Medicine Engineering and Technology of Ministry of Health of The People's Republic of China, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China
  • Pages: 1499-1505
    |
    Published online on: January 29, 2016
       https://doi.org/10.3892/etm.2016.3034
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Abstract

Gynostemma pentaphyllum is a traditional Chinese medicine reported to possess a wide range of health benefits. As the major component of G. pentaphyllum, gypenoside (GP) displays various anti‑inflammatory and anti‑oxidant properties. However, it is unclear whether GP can protect against ischemia/reperfusion (I/R)‑induced renal injury, and the underlying molecular mechanisms associated with this process remain unknown. In the present study, a renal I/R injury model in C57BL/6 mice was established. It was observed that, following I/R, serum concentrations of creatinine (Cr) and blood urea nitrogen (BUN) were significantly increased (P<0.01), indicating renal injury. Pretreatment with GP (50 mg/kg) significantly inhibited I/R‑induced upregulation of serum Cr and BUN (P<0.01). Furthermore, renal malondialdehyde levels were significantly reduced in the I/R+GP group, compared with the I/R group (P<0.01), whereas renal tissue superoxide dismutase activity was significantly higher in the I/R+GP group compared with the I/R group (P<0.01). Further investigation demonstrated that pretreatment with GP produced inhibitory effects on the I/R‑induced production of pro‑inflammatory cytokines, including interleukin (IL)‑1β, IL‑6, tumor necrosis factor‑α and interferon‑γ (P<0.01). In addition, heme oxygenase 1 (HO‑1) expression levels were significantly increased in the I/R group compared with the control (P<0.01), indicating the presence of oxidative damage. However, the I/R‑induced upregulation of HO‑1 was significantly attenuated by pretreatment with GP (P<0.01), which also suppressed I/R‑induced apoptosis by inhibiting pro‑apoptotic Bax and upregulating anti‑apoptotic Bcl‑2 in renal cells (P<0.01). Finally, the activity of ERK signaling was significantly increased in the I/R+GP group compared with the I/R group (P<0.05), which may be associated with the protective effect of GP against I/R‑induced renal cell apoptosis. To conclude, the present results suggest that GP produces a protective effect against I/R‑induced renal injury as a result of its anti‑inflammatory and anti‑apoptotic properties.
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Copy and paste a formatted citation
Spandidos Publications style
Ye Q, Zhu Y, Ye S, Liu H, She X, Niu Y and Ming Y: Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling. Exp Ther Med 11: 1499-1505, 2016.
APA
Ye, Q., Zhu, Y., Ye, S., Liu, H., She, X., Niu, Y., & Ming, Y. (2016). Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling. Experimental and Therapeutic Medicine, 11, 1499-1505. https://doi.org/10.3892/etm.2016.3034
MLA
Ye, Q., Zhu, Y., Ye, S., Liu, H., She, X., Niu, Y., Ming, Y."Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling". Experimental and Therapeutic Medicine 11.4 (2016): 1499-1505.
Chicago
Ye, Q., Zhu, Y., Ye, S., Liu, H., She, X., Niu, Y., Ming, Y."Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1499-1505. https://doi.org/10.3892/etm.2016.3034
Copy and paste a formatted citation
x
Spandidos Publications style
Ye Q, Zhu Y, Ye S, Liu H, She X, Niu Y and Ming Y: Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling. Exp Ther Med 11: 1499-1505, 2016.
APA
Ye, Q., Zhu, Y., Ye, S., Liu, H., She, X., Niu, Y., & Ming, Y. (2016). Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling. Experimental and Therapeutic Medicine, 11, 1499-1505. https://doi.org/10.3892/etm.2016.3034
MLA
Ye, Q., Zhu, Y., Ye, S., Liu, H., She, X., Niu, Y., Ming, Y."Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling". Experimental and Therapeutic Medicine 11.4 (2016): 1499-1505.
Chicago
Ye, Q., Zhu, Y., Ye, S., Liu, H., She, X., Niu, Y., Ming, Y."Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1499-1505. https://doi.org/10.3892/etm.2016.3034
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