Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling

Ye,Qifa; Zhu,Yi; Ye,Shaojun; Liu,Hong; She,Xingguo; Niu,Ying; Ming,Yingzi

doi:10.3892/etm.2016.3034

Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling

Authors:
- Qifa Ye
- Yi Zhu
- Shaojun Ye
- Hong Liu
- Xingguo She
- Ying Niu
- Yingzi Ming
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Affiliations: Center of Transplant Medicine Engineering and Technology of Ministry of Health of The People's Republic of China, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China
Published online on: January 29, 2016 https://doi.org/10.3892/etm.2016.3034
Pages: 1499-1505

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Abstract

Gynostemma pentaphyllum is a traditional Chinese medicine reported to possess a wide range of health benefits. As the major component of G. pentaphyllum, gypenoside (GP) displays various anti‑inflammatory and anti‑oxidant properties. However, it is unclear whether GP can protect against ischemia/reperfusion (I/R)‑induced renal injury, and the underlying molecular mechanisms associated with this process remain unknown. In the present study, a renal I/R injury model in C57BL/6 mice was established. It was observed that, following I/R, serum concentrations of creatinine (Cr) and blood urea nitrogen (BUN) were significantly increased (P<0.01), indicating renal injury. Pretreatment with GP (50 mg/kg) significantly inhibited I/R‑induced upregulation of serum Cr and BUN (P<0.01). Furthermore, renal malondialdehyde levels were significantly reduced in the I/R+GP group, compared with the I/R group (P<0.01), whereas renal tissue superoxide dismutase activity was significantly higher in the I/R+GP group compared with the I/R group (P<0.01). Further investigation demonstrated that pretreatment with GP produced inhibitory effects on the I/R‑induced production of pro‑inflammatory cytokines, including interleukin (IL)‑1β, IL‑6, tumor necrosis factor‑α and interferon‑γ (P<0.01). In addition, heme oxygenase 1 (HO‑1) expression levels were significantly increased in the I/R group compared with the control (P<0.01), indicating the presence of oxidative damage. However, the I/R‑induced upregulation of HO‑1 was significantly attenuated by pretreatment with GP (P<0.01), which also suppressed I/R‑induced apoptosis by inhibiting pro‑apoptotic Bax and upregulating anti‑apoptotic Bcl‑2 in renal cells (P<0.01). Finally, the activity of ERK signaling was significantly increased in the I/R+GP group compared with the I/R group (P<0.05), which may be associated with the protective effect of GP against I/R‑induced renal cell apoptosis. To conclude, the present results suggest that GP produces a protective effect against I/R‑induced renal injury as a result of its anti‑inflammatory and anti‑apoptotic properties.

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