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Effect of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome

  • Authors:
    • Xiumei Liu
    • Xueming Wang
    • Aimin Li
    • Xiaoling Jiao
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1211-1216
    |
    Published online on: February 9, 2016
       https://doi.org/10.3892/etm.2016.3059
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Abstract

The aim of the present study was to investigate the effect of bone marrow mesenchymal stem cell (MSC) transplantation on brain‑derived neurotrophic factor (BDNF) expression in the striatum of Tourette syndrome (TS) rats. In addition, the possible mechanism of MSC transplantation in the treatment of TS was investigated. A total of 72 Wistar rats were randomly allocated into the control (sham surgery) group and the two experimental groups, including the TS+vehicle and TS+MSC. MSCs were co‑cultured with 5‑bromodeoxyuridine for 24 h for labeling prior to grafting. An autoimmune TS rat model was successfully established in the present study. Rat MSCs were cultured and expanded using density gradient centrifugation in vitro, identified by flow cytometry and then transplanted into the striata of the TS+MSC group rats. The mRNA and protein expression levels of BDNF were detected by RT‑qPCR and ELISA, respectively. The results indicated that the stereotypic behavior of TS rats was reduced 7 days after MSC transplantation, while the mRNA and protein BDNF levels in the striatum increased, compared with the sham surgery group (P<0.05). In addition, the BDNF mRNA and protein expression level was lower in the striatum of TS+MSC transplantation, compared with that in TS+vehicle rats. In conclusion, intrastriatal transplantation of MSCs may provide relief from stereotypic TS behavior, since the BDNF level was reduced in TS rats after MSC transplantation.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Wang X, Li A and Jiao X: Effect of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome. Exp Ther Med 11: 1211-1216, 2016.
APA
Liu, X., Wang, X., Li, A., & Jiao, X. (2016). Effect of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome. Experimental and Therapeutic Medicine, 11, 1211-1216. https://doi.org/10.3892/etm.2016.3059
MLA
Liu, X., Wang, X., Li, A., Jiao, X."Effect of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome". Experimental and Therapeutic Medicine 11.4 (2016): 1211-1216.
Chicago
Liu, X., Wang, X., Li, A., Jiao, X."Effect of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1211-1216. https://doi.org/10.3892/etm.2016.3059
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Wang X, Li A and Jiao X: Effect of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome. Exp Ther Med 11: 1211-1216, 2016.
APA
Liu, X., Wang, X., Li, A., & Jiao, X. (2016). Effect of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome. Experimental and Therapeutic Medicine, 11, 1211-1216. https://doi.org/10.3892/etm.2016.3059
MLA
Liu, X., Wang, X., Li, A., Jiao, X."Effect of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome". Experimental and Therapeutic Medicine 11.4 (2016): 1211-1216.
Chicago
Liu, X., Wang, X., Li, A., Jiao, X."Effect of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1211-1216. https://doi.org/10.3892/etm.2016.3059
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