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Article Open Access

Hepatoprotective effect of resveratrol against ethanol-induced oxidative stress through induction of superoxide dismutase in vivo and in vitro

  • Authors:
    • Wei‑Ming Chen
    • Lee‑Hsin Shaw
    • Pey‑Jium Chang
    • Shui‑Yi Tung
    • Te‑Sheng Chang
    • Chein‑Heng Shen
    • Yung‑Yu Hsieh
    • Kuo‑Liang Wei
  • View Affiliations / Copyright

    Affiliations: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chang Gung Memorial Hospital, Puzi, Chiayi 61363, Taiwan, R.O.C., Institute of Traditional Medicine, National Yang‑Ming University, Taipei 11221, Taiwan, R.O.C., Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan, R.O.C.
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1231-1238
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    Published online on: February 16, 2016
       https://doi.org/10.3892/etm.2016.3077
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Abstract

The present study aimed to investigate the hepatoprotective effect of resveratrol (RSV) against ethanol-induced oxidative stress in vivo, and investigate the underlying mechanisms by which RSV exerts its anti‑oxidative effects on hepatic cells. C57BL/6J mice were divided into four groups: Untreated control, ethanol-treated, RSV-treated, and ethanol + RSV‑treated. The plasma lipid profile, hepatic lipid accumulation and antioxidative enzyme activities were analyzed. HepG2 cells were used as a cellular model to analyze the effects of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and peroxisome proliferator-activated receptors (PPARs) in the RSV‑mediated protection of ethanol‑induced oxidative stress. In C57BL/6J mice, ethanol caused a significant increase in plasma triglyceride levels and hepatic lipid accumulation (P<0.05), whereas RSV notably increased SOD activity. In HepG2 cells, SOD activity was enhanced in the RSV‑treated HepG2 cells, whereas the activity of CAT and GPx was not affected. Western blot and quantitative polymerase chain reaction analyses demonstrated that RSV significantly increased SOD protein and mRNA expression levels (P<0.05). Using a transient transfection assay, PPARγ was observed to participate in the regulation of SOD gene expression in RSV-administered HepG2 cells. To conclude, the results from the present study suggest that RSV may contribute towards the protection of hepatic cells from ethanol‑induced oxidative stress via the induction of SOD activity and gene expression.
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Copy and paste a formatted citation
Spandidos Publications style
Chen WM, Shaw LH, Chang PJ, Tung SY, Chang TS, Shen CH, Hsieh YY and Wei KL: Hepatoprotective effect of resveratrol against ethanol-induced oxidative stress through induction of superoxide dismutase in vivo and in vitro. Exp Ther Med 11: 1231-1238, 2016.
APA
Chen, W., Shaw, L., Chang, P., Tung, S., Chang, T., Shen, C. ... Wei, K. (2016). Hepatoprotective effect of resveratrol against ethanol-induced oxidative stress through induction of superoxide dismutase in vivo and in vitro. Experimental and Therapeutic Medicine, 11, 1231-1238. https://doi.org/10.3892/etm.2016.3077
MLA
Chen, W., Shaw, L., Chang, P., Tung, S., Chang, T., Shen, C., Hsieh, Y., Wei, K."Hepatoprotective effect of resveratrol against ethanol-induced oxidative stress through induction of superoxide dismutase in vivo and in vitro". Experimental and Therapeutic Medicine 11.4 (2016): 1231-1238.
Chicago
Chen, W., Shaw, L., Chang, P., Tung, S., Chang, T., Shen, C., Hsieh, Y., Wei, K."Hepatoprotective effect of resveratrol against ethanol-induced oxidative stress through induction of superoxide dismutase in vivo and in vitro". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1231-1238. https://doi.org/10.3892/etm.2016.3077
Copy and paste a formatted citation
x
Spandidos Publications style
Chen WM, Shaw LH, Chang PJ, Tung SY, Chang TS, Shen CH, Hsieh YY and Wei KL: Hepatoprotective effect of resveratrol against ethanol-induced oxidative stress through induction of superoxide dismutase in vivo and in vitro. Exp Ther Med 11: 1231-1238, 2016.
APA
Chen, W., Shaw, L., Chang, P., Tung, S., Chang, T., Shen, C. ... Wei, K. (2016). Hepatoprotective effect of resveratrol against ethanol-induced oxidative stress through induction of superoxide dismutase in vivo and in vitro. Experimental and Therapeutic Medicine, 11, 1231-1238. https://doi.org/10.3892/etm.2016.3077
MLA
Chen, W., Shaw, L., Chang, P., Tung, S., Chang, T., Shen, C., Hsieh, Y., Wei, K."Hepatoprotective effect of resveratrol against ethanol-induced oxidative stress through induction of superoxide dismutase in vivo and in vitro". Experimental and Therapeutic Medicine 11.4 (2016): 1231-1238.
Chicago
Chen, W., Shaw, L., Chang, P., Tung, S., Chang, T., Shen, C., Hsieh, Y., Wei, K."Hepatoprotective effect of resveratrol against ethanol-induced oxidative stress through induction of superoxide dismutase in vivo and in vitro". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1231-1238. https://doi.org/10.3892/etm.2016.3077
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