Role of microRNA‑210 in human intervertebral disc degeneration Retraction in /10.3892/etm.2023.12210

Zhang,Da‑Ying; Wang,Zhi‑Jian; Yu,Yan‑Bo; Zhang,Yong; Zhang,Xue‑Xue

doi:10.3892/etm.2016.3176

Role of microRNA‑210 in human intervertebral disc degeneration

Retraction in: /10.3892/etm.2023.12210

Authors:
- Da‑Ying Zhang
- Zhi‑Jian Wang
- Yan‑Bo Yu
- Yong Zhang
- Xue‑Xue Zhang
View Affiliations

Affiliations: Department of Pain Management, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
Published online on: March 21, 2016 https://doi.org/10.3892/etm.2016.3176
Pages: 2349-2354

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The present study aimed to investigate the role of microRNA (miR)‑210 in the development of intervertebral disc degeneration (IDD). Human nucleus pulposus (NP) samples were collected from patients with scoliosis and IDD (n=12 each) as the scoliosis control and IDD groups, respectively. The expression levels of miR‑210 were detected using reverse‑transcription quantitative polymerase chain reaction. In vitro overexpression and knockdown of miR‑210 in human NP cells were achieved by transfection of NP cells with lentiviral pre‑miR‑210 and antagomiR‑210, respectively. The protein expression levels of homeobox A9 (HOXA9) were then detected in NP cells with modulated miR‑210 using western blot analysis. Flow cytometry with allophycocyanin‑Annexin V/7 and 7‑aminoactinomycin D staining was also used to detect the proportion of NP cells with modulated miR‑210 undergoing apoptosis. The current study revealed that the miR‑210 expression was decreased in patients with IDD compared with that of the scoliosis control group (P<0.05). Furthermore, the upregulation of miR‑210 with pre‑miR‑210 led to the repression of HOXA9. The HOXA9 level was significantly lower in these cells compared with that of NP cells treated with a corresponding negative sequence (P<0.05). Knockdown of miR‑210 with antagomiR‑210 resulted in upregulation of HOXA9 in NP cells, determined as the level of HOXA9 was significantly higher than that of NP cells treated with a negative sequence (P<0.05). The proportion of apoptotic NP cells also significantly decreased following treatment with pre‑miR‑210 compared with the scoliosis control group (12.1±1.43 vs. 23.8±1.22%, respectively; P<0.05). In conclusion, downregulation of miR‑210 may promote Fas‑mediated apoptosis in human IDD by regulating the expression of HOXA9. This indicates that miR‑210 may be closely associated with the development of IDD and may act as a novel target in IDD treatment.

View Figures

View References

1	Sudo H, Yamada K, Iwasaki K, Higashi H, Ito M, Minami A and Iwasaki N: Global identification of genes related to nutrient deficiency in intervertebral disc cells in an experimental nutrient deprivation model. PLoS One. 8:e588062013. View Article : Google Scholar : PubMed/NCBI
2	Song YQ, Karasugi T, Cheung KM, Chiba K, Ho DW, Miyake A, Kao PY, Sze KL, Yee A, Takahashi A, et al: Lumbar disc degeneration is linked to a carbohydrate sulfotransferase 3 variant. J Clin Invest. 123:4909–4917. 2013. View Article : Google Scholar : PubMed/NCBI
3	Wang YX, Griffith JF, Zeng XJ, Deng M, Kwok AW, Leung JC, Ahuja AT, Kwok T and Leung PC: Prevalence and sex difference of lumbar disc space narrowing in elderly Chinese men and women: Osteoporotic fractures in men (Hong Kong) and osteoporotic fractures in women (Hong Kong) studies. Arthritis Rheum. 65:1004–1010. 2013. View Article : Google Scholar : PubMed/NCBI
4	Wang YBMC: Epidemiology of lumbar disc degeneration. The intervertebral disc. 139–156. 2014. View Article : Google Scholar
5	Liu G, Cao P, Chen H, Yuan W, Wang J and Tang X: miR-27a regulates apoptosis in nucleus pulposus cells by targeting PI3K. PLoS One. 8:e752512013. View Article : Google Scholar : PubMed/NCBI
6	Guehring T, Wilde G, Sumner M, Grünhagen T, Karney GB, Tirlapur UK and Urban JP: Notochordal intervertebral disc cells: Sensitivity to nutrient deprivation. Arthritis Rheum. 60:1026–1034. 2009. View Article : Google Scholar : PubMed/NCBI
7	Wang YX and Griffith JF: Menopause causes vertebral endplate degeneration and decrease in nutrient diffusion to the intervertebral discs. Med Hypotheses. 77:18–20. 2011. View Article : Google Scholar : PubMed/NCBI
8	Takatalo J, Karppinen J, Taimela S, Niinimäki J, Laitinen J, Sequeiros Blanco R, Paananen M, Remes J, Näyhä S, Tammelin T, et al: Body mass index is associated with lumbar disc degeneration in young Finnish males: Subsample of Northern Finland birth cohort study 1986. BMC Musculoskelet Disord. 14:872013. View Article : Google Scholar : PubMed/NCBI
9	Omair A, Holden M, Lie BA, Reikeras O and Brox JI: Treatment outcome of chronic low back pain and radiographic lumbar disc degeneration are associated with inflammatory and matrix degrading gene variants: A prospective genetic association study. BMC Musculoskelet Disord. 14:1052013. View Article : Google Scholar : PubMed/NCBI
10	Wang HQ, Yu XD, Liu ZH, Cheng X, Samartzis D, Jia LT, Wu SX, Huang J, Chen J and Luo ZJ: Deregulated miR-155 promotes Fas-mediated apoptosis in human intervertebral disc degeneration by targeting FADD and caspase-3. J Pathol. 225:232–242. 2011. View Article : Google Scholar : PubMed/NCBI
11	Wang W, Kwon EJ and Tsai LH: MicroRNAs in learning, memory and neurological diseases. Learn Mem. 19:359–368. 2012. View Article : Google Scholar : PubMed/NCBI
12	McDermott AM, Heneghan HM, Miller N and Kerin MJ: The therapeutic potential of microRNAs: Disease modulators and drug targets. Pharm Res. 28:3016–3029. 2011. View Article : Google Scholar : PubMed/NCBI
13	Grosso S, Doyen J, Parks SK, Bertero T, Paye A, Cardinaud B, Gounon P, Lacas-Gervais S, Noël A, Pouysségur J, et al: MiR-210 promotes a hypoxic phenotype and increases radioresistance in human lung cancer cell lines. Cell Death Dis. 4:e5442013. View Article : Google Scholar : PubMed/NCBI
14	Obad S, dos Santos CO, Petri A, Heidenblad M, Broom O, Ruse C, Fu C, Lindow M, Stenvang J, Straarup EM, et al: Silencing of microRNA families by seed-targeting tiny LNAs. Nat Genet. 43:371–378. 2011. View Article : Google Scholar : PubMed/NCBI
15	Mei Y, Gao C, Wang K, Cui L, Li W, Zhao X, Liu F, Wu M, Deng G, Ding W, et al: Effect of microRNA-210 on prognosis and response to chemotherapeutic drugs in pediatric acute lymphoblastic leukemia. Cancer Sci. 105:463–472. 2014. View Article : Google Scholar : PubMed/NCBI
16	Devlin C, Greco S, Martelli F and Ivan M: miR-210: More than a silent player in hypoxia. IUBMB Life. 63:94–100. 2011.PubMed/NCBI
17	Camacho L, Guerrero P and Marchetti D: MicroRNA and protein profiling of brain metastasis competent cell-derived exosomes. PLoS One. 8:e737902013. View Article : Google Scholar : PubMed/NCBI
18	Chang KP and Lai CS: Micro-RNA profiling as biomarkers in flap ischemia-reperfusion injury. Microsurgery. 32:642–648. 2012. View Article : Google Scholar : PubMed/NCBI
19	Cui J, Eldredge JB, Xu Y and Puett D: MicroRNA expression and regulation in human ovarian carcinoma cells by luteinizing hormone. PLoS One. 6:e217302011. View Article : Google Scholar : PubMed/NCBI
20	Cheng HH, Mitchell PS, Kroh EM, Dowell AE, Chéry L, Siddiqui J, Nelson PS, Vessella RL, Knudsen BS, Chinnaiyan AM, et al: Circulating microRNA profiling identifies a subset of metastatic prostate cancer patients with evidence of cancer-associated hypoxia. PLoS One. 8:e692392013. View Article : Google Scholar : PubMed/NCBI
21	Hamama S, Noman MZ, Gervaz P, Delanian S and Vozenin MC: MiR-210: A potential therapeutic target against radiation-induced enteropathy. Radiother Oncol. 111:219–221. 2014. View Article : Google Scholar : PubMed/NCBI
22	Kong J, Ma X, Wang T, Ma J, Tian P, Han C, Zang J, Li P and Jiang H: Research progress of Wnt/beta-catenin and nuclear factor-kappa B pathways and their relevance to intervertebral disc degeneration. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 27:1523–1528. 2013.(In Chinese). PubMed/NCBI
23	No authors listed. The Helsinki Declaration of the World Medical Association (WMA). Ethical principles of medical research involving human subjects. Pol Merkur Lekarski. 36:298–301. 2014.(In Polish). PubMed/NCBI
24	Pfirrmann CW, Metzdorf A, Zanetti M, Hodler J and Boos N: Magnetic resonance classification of lumbar intervertebral disc degeneration. Spine (Phila Pa 1976). 26:1873–1878. 2001. View Article : Google Scholar : PubMed/NCBI
25	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2^−ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
26	Hirata H, Ueno K, Shahryari V, Tanaka Y, Tabatabai ZL, Hinoda Y and Dahiya R: Oncogenic miRNA-182-5p targets Smad4 and RECK in human bladder cancer. PLoS One. 7:e510562012. View Article : Google Scholar : PubMed/NCBI
27	Ruepp A, Kowarsch A and Theis F: PhenomiR: microRNAs in human diseases and biological processes. Methods Mol Biol. 822:249–260. 2012. View Article : Google Scholar : PubMed/NCBI
28	Ha TY: MicroRNAs in Human Diseases: From Lung, Liver and Kidney Diseases to Infectious Disease, Sickle Cell Disease and Endometrium Disease. Immune Netw. 11:309–323. 2011. View Article : Google Scholar : PubMed/NCBI
29	Yu X, Li Z, Shen J, Wu WK, Liang J, Weng X and Qiu G: MicroRNA-10b promotes nucleus pulposus cell proliferation through RhoC-Akt pathway by targeting HOXD10 in intervetebral disc degeneration. PLoS One. 8:e830802013. View Article : Google Scholar : PubMed/NCBI
30	Mathew LK and Simon MC: mir-210: A sensor for hypoxic stress during tumorigenesis. Mol Cell. 35:737–738. 2009. View Article : Google Scholar : PubMed/NCBI
31	Hong L, Yang J, Han Y, Lu Q, Cao J and Syed L: High expression of miR-210 predicts poor survival in patients with breast cancer: A meta-analysis. Gene. 507:135–138. 2012. View Article : Google Scholar : PubMed/NCBI
32	Ivan M and Huang X: miR-210: Fine-tuning the hypoxic response. Adv Exp Med Biol. 772:205–227. 2014. View Article : Google Scholar : PubMed/NCBI
33	Hong L, Han Y, Zhang H, Zhao Q and Qiao Y: miR-210: A therapeutic target in cancer. Expert Opin Ther Targets. 17:21–28. 2013. View Article : Google Scholar : PubMed/NCBI
34	Thurner EM, Krenn-Pilko S, Langsenlehner U, Renner W, Gerger A, Kapp KS and Langsenlehner T: Association of genetic variants in apoptosis genes FAS and FASL with radiation-induced late toxicity after prostate cancer radiotherapy. Strahlenther Onkol. 190:304–309. 2014. View Article : Google Scholar : PubMed/NCBI
35	Han W, Zhou Y, Zhong R, Wu C, Song R, Liu L, Zou L, Qiao Y, Zhai K, Chang J, et al: Functional polymorphisms in FAS/FASL system increase the risk of neuroblastoma in Chinese population. PLoS One. 8:e716562013. View Article : Google Scholar : PubMed/NCBI
36	Roberts S, Evans H, Trivedi J and Menage J: Histology and pathology of the human intervertebral disc. J Bone Joint Surg Am. 88(Suppl 2): 10–14. 2006. View Article : Google Scholar : PubMed/NCBI