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Article

Role of microRNA‑210 in human intervertebral disc degeneration

Retraction in: /10.3892/etm.2023.12210
  • Authors:
    • Da‑Ying Zhang
    • Zhi‑Jian Wang
    • Yan‑Bo Yu
    • Yong Zhang
    • Xue‑Xue Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Pain Management, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
  • Pages: 2349-2354
    |
    Published online on: March 21, 2016
       https://doi.org/10.3892/etm.2016.3176
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Abstract

The present study aimed to investigate the role of microRNA (miR)‑210 in the development of intervertebral disc degeneration (IDD). Human nucleus pulposus (NP) samples were collected from patients with scoliosis and IDD (n=12 each) as the scoliosis control and IDD groups, respectively. The expression levels of miR‑210 were detected using reverse‑transcription quantitative polymerase chain reaction. In vitro overexpression and knockdown of miR‑210 in human NP cells were achieved by transfection of NP cells with lentiviral pre‑miR‑210 and antagomiR‑210, respectively. The protein expression levels of homeobox A9 (HOXA9) were then detected in NP cells with modulated miR‑210 using western blot analysis. Flow cytometry with allophycocyanin‑Annexin V/7 and 7‑aminoactinomycin D staining was also used to detect the proportion of NP cells with modulated miR‑210 undergoing apoptosis. The current study revealed that the miR‑210 expression was decreased in patients with IDD compared with that of the scoliosis control group (P<0.05). Furthermore, the upregulation of miR‑210 with pre‑miR‑210 led to the repression of HOXA9. The HOXA9 level was significantly lower in these cells compared with that of NP cells treated with a corresponding negative sequence (P<0.05). Knockdown of miR‑210 with antagomiR‑210 resulted in upregulation of HOXA9 in NP cells, determined as the level of HOXA9 was significantly higher than that of NP cells treated with a negative sequence (P<0.05). The proportion of apoptotic NP cells also significantly decreased following treatment with pre‑miR‑210 compared with the scoliosis control group (12.1±1.43 vs. 23.8±1.22%, respectively; P<0.05). In conclusion, downregulation of miR‑210 may promote Fas‑mediated apoptosis in human IDD by regulating the expression of HOXA9. This indicates that miR‑210 may be closely associated with the development of IDD and may act as a novel target in IDD treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang DY, Wang ZJ, Yu YB, Zhang Y and Zhang XX: Role of microRNA‑210 in human intervertebral disc degeneration Retraction in /10.3892/etm.2023.12210. Exp Ther Med 11: 2349-2354, 2016.
APA
Zhang, D., Wang, Z., Yu, Y., Zhang, Y., & Zhang, X. (2016). Role of microRNA‑210 in human intervertebral disc degeneration Retraction in /10.3892/etm.2023.12210. Experimental and Therapeutic Medicine, 11, 2349-2354. https://doi.org/10.3892/etm.2016.3176
MLA
Zhang, D., Wang, Z., Yu, Y., Zhang, Y., Zhang, X."Role of microRNA‑210 in human intervertebral disc degeneration Retraction in /10.3892/etm.2023.12210". Experimental and Therapeutic Medicine 11.6 (2016): 2349-2354.
Chicago
Zhang, D., Wang, Z., Yu, Y., Zhang, Y., Zhang, X."Role of microRNA‑210 in human intervertebral disc degeneration Retraction in /10.3892/etm.2023.12210". Experimental and Therapeutic Medicine 11, no. 6 (2016): 2349-2354. https://doi.org/10.3892/etm.2016.3176
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang DY, Wang ZJ, Yu YB, Zhang Y and Zhang XX: Role of microRNA‑210 in human intervertebral disc degeneration Retraction in /10.3892/etm.2023.12210. Exp Ther Med 11: 2349-2354, 2016.
APA
Zhang, D., Wang, Z., Yu, Y., Zhang, Y., & Zhang, X. (2016). Role of microRNA‑210 in human intervertebral disc degeneration Retraction in /10.3892/etm.2023.12210. Experimental and Therapeutic Medicine, 11, 2349-2354. https://doi.org/10.3892/etm.2016.3176
MLA
Zhang, D., Wang, Z., Yu, Y., Zhang, Y., Zhang, X."Role of microRNA‑210 in human intervertebral disc degeneration Retraction in /10.3892/etm.2023.12210". Experimental and Therapeutic Medicine 11.6 (2016): 2349-2354.
Chicago
Zhang, D., Wang, Z., Yu, Y., Zhang, Y., Zhang, X."Role of microRNA‑210 in human intervertebral disc degeneration Retraction in /10.3892/etm.2023.12210". Experimental and Therapeutic Medicine 11, no. 6 (2016): 2349-2354. https://doi.org/10.3892/etm.2016.3176
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