Effect of electroacupuncture on gastric interstitial cells of Cajal in a rat model of diabetic gastroparesis

Lin,Guohua; Zhang,Jiawei; Li,Lixia; Zou,Zhuocheng; Chen,Chuyun; Xue,Lifei; Zhao,Lanfeng

doi:10.3892/etm.2016.3185

Effect of electroacupuncture on gastric interstitial cells of Cajal in a rat model of diabetic gastroparesis

Authors:
- Guohua Lin
- Jiawei Zhang
- Lixia Li
- Zhuocheng Zou
- Chuyun Chen
- Lifei Xue
- Lanfeng Zhao
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Affiliations: Department of Acupuncture, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China, College of Acupuncture and Moxibustion, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China, Department of Acupuncture, Guangzhou Hospital of Traditional Chinese Medicine, Guangzhou, Guangdong 510130, P.R. China, Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530023, P.R. China
Published online on: March 24, 2016 https://doi.org/10.3892/etm.2016.3185
Pages: 2489-2494

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Abstract

The aim of the present study was to observe the effect of electroacupuncture (EA) on the gastric interstitial cells of Cajal (ICCs) in a rat model of diabetic gastroparesis (DGP). The gastric tissues were collected from 75 rats, which had been divided into three equal groups (n=25/group): Blank, model and EA. Hematoxylin and eosin and immunohistochemical staining were used to observe the cellular morphology and distribution of c‑kit‑positive gastric ICCs; light microscopy was used to count the number of ICCs; and electron microscopy was used to observe the ultrastructure of the rat ICCs. Compared with the model group, the gastromucosal glandular and smooth muscle cells of the EA group were more regularly arranged, with fewer vacuoles; there was an increased cellular gap and the vacuolar degeneration on the gastric walls was mild. Image analysis showed that the blank group exhibited the greatest number of c‑kit‑positive ICCs, and the number of c‑kit‑positive ICCs in the blank group was significantly different from that in the model and EA groups (P<0.01): Blank group > EA group > model group. In conclusion, DGP rats exhibited a reduced number of gastric ICCs, altered ultrastructural morphology and a reduced number of cell organelles, particularly mitochondria, compared with the blank group. EA may help to reverse the various pathological changes of gastric ICCs in rat models of DGP.

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