Open Access

MicroRNA‑223 promotes mast cell apoptosis by targeting the insulin‑like growth factor 1 receptor

  • Authors:
    • Haiyan Gao
    • Huan Deng
    • Hong Xu
    • Qianyuan Yang
    • Yao Zhou
    • Jiamin Zhang
    • Deyu Zhao
    • Feng Liu
  • View Affiliations

  • Published online on: April 5, 2016     https://doi.org/10.3892/etm.2016.3227
  • Pages: 2171-2176
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to examine the functional role of miR‑223 in the regulation of mast cell apoptosis. Overexpressed miR‑223 in mast cells transfected by Lipofectamine 2000 was used as a model, and miR‑223 was found to promote mast cell apoptosis. To investigate the underlying mechanisms involved, the potential and putative target molecules of miR‑223 were detected by bioinformatical analysis using predictive software, and western blotting. Insulin‑like growth factor‑1 receptor (IGF‑1R) was found to be the functional target of miR‑223 in the promotion of cell apoptosis. The downstream PI3K/protein kinase B (Akt) signaling pathway was also inhibited, and signaling was mediated by IGF‑1R. Furthermore, the relative luciferase activity of the reporter containing the 3'‑untranslated region (3'‑UTR) of IGF‑1R was significantly suppressed, while suppression of miR‑223‑inhibited IGF‑1R protein expression was also observed. In conclusion, the results suggest that IGF‑1R is the functional target for miR‑223 promotion of cell apoptosis, and its downstream PI3K/Akt signaling pathway was suppressed by miR‑223 through targeting of IGF‑1R.
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June-2016
Volume 11 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Gao H, Deng H, Xu H, Yang Q, Zhou Y, Zhang J, Zhao D and Liu F: MicroRNA‑223 promotes mast cell apoptosis by targeting the insulin‑like growth factor 1 receptor. Exp Ther Med 11: 2171-2176, 2016
APA
Gao, H., Deng, H., Xu, H., Yang, Q., Zhou, Y., Zhang, J. ... Liu, F. (2016). MicroRNA‑223 promotes mast cell apoptosis by targeting the insulin‑like growth factor 1 receptor. Experimental and Therapeutic Medicine, 11, 2171-2176. https://doi.org/10.3892/etm.2016.3227
MLA
Gao, H., Deng, H., Xu, H., Yang, Q., Zhou, Y., Zhang, J., Zhao, D., Liu, F."MicroRNA‑223 promotes mast cell apoptosis by targeting the insulin‑like growth factor 1 receptor". Experimental and Therapeutic Medicine 11.6 (2016): 2171-2176.
Chicago
Gao, H., Deng, H., Xu, H., Yang, Q., Zhou, Y., Zhang, J., Zhao, D., Liu, F."MicroRNA‑223 promotes mast cell apoptosis by targeting the insulin‑like growth factor 1 receptor". Experimental and Therapeutic Medicine 11, no. 6 (2016): 2171-2176. https://doi.org/10.3892/etm.2016.3227