Effects of Linderae radix extracts on a rat model of alcoholic liver injury

Wang,Jun‑Wei; Chen,Xin‑Yi; Hu,Pei‑Yang; Tan,Ming‑Ming; Tang,Xiao‑Gang; Huang,Min‑Cong; Lou,Zhao‑Huan

doi:10.3892/etm.2016.3244

Effects of Linderae radix extracts on a rat model of alcoholic liver injury

Authors:
- Jun‑Wei Wang
- Xin‑Yi Chen
- Pei‑Yang Hu
- Ming‑Ming Tan
- Xiao‑Gang Tang
- Min‑Cong Huang
- Zhao‑Huan Lou
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Affiliations: Emergency Department, People's Hospital of Tiantai County, Tiantai, Zhejiang 317200, P.R. China, Safety Evaluation Center, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310007, P.R. China, Institute of Material Medica, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China
Published online on: April 11, 2016 https://doi.org/10.3892/etm.2016.3244
Pages: 2185-2192
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Traditional treatments have a poor effect on alcoholic liver diseases. Linderae radix (LR), the dried root of Lindera aggregata (Sims) Kosterm., has been frequently used in traditional Chinese medicine for treating various diseases, and has been shown to exhibit a protective effect on liver injury. In the present study, LR extracts were made using various solvents, and then administrated to rats to establish a model of ethanol‑induced liver injury. The study aimed to investigate the therapeutic effects and potential mechanism of LR extracts on acute alcoholic liver injury. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycercide (TG), cholesterol (TC), methane dicarboxylic aldehyde (MDA) and superoxide dismutase (SOD) were determined using an automatic biochemistry analyzer. In addition, pathological examination was performed by hematoxylin‑eosin staining. The levels of MDA and SOD, and the expression levels of nuclear factor (NF)‑κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver tissue were investigated immunohistochemically. The expression of cytochrome P450 2E1 (CYP2E1) mRNA was quantified by reverse transcription‑quantitative polymerase chain reaction. The results indicated that LR extracts improved the histopathological status and decreased the serum levels of ALT, AST, TG, TC and MDA. Furthermore, the levels of MDA and inflammatory mediators (NF‑κB, TNF‑α and IL‑1β) were decreased in liver tissues, and the overexpression of CYP2E1 mRNA induced by ethanol treatment. LR extracts exhibited a protective effect on alcoholic liver injury and the mechanism may be associated with the anti-inflammatory and anti-oxidative action.

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