Chinese herbal formula QHF inhibits liver cancer cell invasion and migration Retraction in /10.3892/etm.2025.12890

Chen,Tao; Wang,Quan; Li,Yunxiao; Huang,Hefei; Hu,Wei

doi:10.3892/etm.2016.3247

June-2016 Volume 11 Issue 6

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June-2016 Volume 11 Issue 6

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Article

Chinese herbal formula QHF inhibits liver cancer cell invasion and migration

Retraction in: /10.3892/etm.2025.12890

Authors:
- Tao Chen
- Quan Wang
- Yunxiao Li
- Hefei Huang
- Wei Hu
View Affiliations / Copyright

Affiliations: College of Medical Sciences, China Three Gorges University, Yichang, Hubei 443002, P.R. China
Pages: 2413-2419
|
Published online on: April 11, 2016

https://doi.org/10.3892/etm.2016.3247
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Abstract

The aim of the present study was to observe the effects of the Chinese herbal formula QHF (Q, Qingrejiedu; H, Huoxuehuayu; and F, Fuzhengguben) on the migration and invasion of hepatocellular carcinoma (HCC) HepG2 cells and to elucidate the potential molecular mechanisms involved. HepG2 cells were treated with various concentrations of QHF, and scratch and Transwell® migration assays were used to qualitatively analyze differences in the migration and invasion activity of these cells. Extracellular signal‑regulated kinase (ERK), p38 and c‑Jun N‑terminal kinase (JNK) inhibitors were subsequently introduced in order to study the association between QHF and the invasion of HepG2 cells. The protein expression levels of the mitogen‑activated protein kinase (MAPK) signaling pathway in HepG2 cells in the presence and absence of QHF were additionally determined using western blot analysis. The results showed that QHF significantly inhibited the proliferation of the HepG2 cells in a concentration‑dependent manner, in addition to inhibiting cell movement, which reduced the ability of the cells to invade and migrate. Western blot analysis indicated that the effects of QHF on HCC HepG2 cells after 24 h were to significantly decrease the expression of phosphorylated‑ (p‑)ERK and to increase the expression of p‑p38 and p‑JNK; however, the total quantity of ERK, p38 and JNK protein remained unchanged. The administration of an inhibitor of ERK altered p38 and JNK expression and promoted the anti‑invasion effects of QHF, whereas p38 and JNK inhibitors only partially reversed this effect. The results of the present study indicate, therefore, that QHF is able to inhibit the migratory and invasive activity of HepG2 cells. A possible underlying mechanism involves the activation of the p38 and JNK MAPK signaling pathway and the attenuation of the ERK signaling pathway.

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1	Chen T, Li D, Fu YL and Hu W: Screening of QHF formula of effective ingredients from Chinese herbs and its anti-hepatic cell cancer effect in combination with chemotherapy. Chinese Medical J (Engl). 121:363–368. 2008.
2	Tao C, Dan L, Ling F and Peng G: In vivo and in vitro effects of QHF combined with chemotherapy on hepatocellular carcinoma. J Biomed Res. 24:161–168. 2010. View Article : Google Scholar : PubMed/NCBI
3	Cong H and Bin H: Effect of traditional Chinese medicine and the active ingredients on apoptosis of liver cells. Zhong Yi Yao Za Zhi. 29:24–26. 2001.
4	Chen T, Fu YL and Gong ZP: QHF compound in combination with small dose of cisplatin inhibit angiogenesis in H22 liver cancer mice. Shi Jie Chang Wei Bing Xue Za Zhi. 18:113–118. 2010.
5	Chen T, Fu YL, Gong ZP, Deng LR and Hu YG: Studies on the anti-angiogenic mechanism of the formula of Chinese medicine active ingredients combined with small dose cisplatin in mice of hepatocellular carcinoma. Zhong Guo Shi Yan Fang Ji Xue Za Zhe She. 16:157–160. 2010.(In Chinese).
6	Xie HJ: Experimental study on the status and prospects of the mechanism of traditional Chinese medicine treatment of liver cancer. J Chin Med Res. 22:62–64. 2009.(In Chinese).
7	Sun HC: Advances in the treatment of postoperative metastasis and recurrence of liver cancer. Ai Zheng Jin Zhan Za Zhe Shi. 3:30–32. 2005.(In Chinese).
8	Song MK, Kim YJ, Song M, Choi HS, Park YK and Ryu JC: Polycyclic aromatic hydrocarbons induce migration in human hepatocellular carcinoma cells (HepG2) through reactive oxygen species-mediated p38 MAPK signal transduction. Cancer Sci. 102:1636–1644. 2011. View Article : Google Scholar : PubMed/NCBI
9	Johansson N, Ala-aho R, Uitto V, Grénman R, Fusenig NE, López-Otín C and Kähäri VM: Expression of collagenase-3 (MMP-13) and collagenase-1 (MMP-1) by transformed keratinocytes is dependent on the activity of p38 mitogen-activated protein kinase. J Cell Sci. 113:227–235. 2000.PubMed/NCBI
10	Liang J, Bao C, Wei J and Su RJ: The expression and significance of p38 and ERK1/2 in hepatocellular carcinoma. Zhong Guo Zu Zhi Hua Xue Yu Xi Bao Hua Xue Za Zhi. 18:202–205. 2009.(In Chinese).
11	Kadowaki S, Endoh D, Okui T and Hayashi M: Trientine, a copper-chelating agent, induced apoptosis in murine fibrosarcoma cells by activation of the p38 MAPK pathway. J Vet Med Sci. 71:1541–1544. 2009. View Article : Google Scholar : PubMed/NCBI
12	Win S, Than TA, Han D, Petrovic LM and Kaplowitz N: c-Jun N-terminal kinase (JNK)-dependent acute liver injury from acetaminophen or tumor necrosis factor (TNF) requires mitochondrial Sab expression in mice. J Biol Chem. 286:35071–35078. 2011. View Article : Google Scholar : PubMed/NCBI
13	Gozdecka M, Lyons S, Kondo S, Taylor J, Li Y, Walczynski J, Thiel G, Breitwieser W and Jones N: JNK suppresses tumor formation via a gene-expression program mediated by ATF2. Cell Rep. 9:1361–1374. 2014. View Article : Google Scholar : PubMed/NCBI
14	Hsieh SC, Tsia JP, Yang SF, Tang MJ and Hsieh YH: Metformin inhibits the invasion of human hepatocellular carcinoma cells and enhances the chemosensitivity to sorafenib through a downregulation of the ERK/JNK-mediated NF-κB dependent pathway that reduces uPA and MMP-9 expression. Amino Acids. 46:2809–2822. 2014. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Chinese herbal formula QHF inhibits liver cancer cell invasion and migration

Retraction in: /10.3892/etm.2025.12890

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Abstract

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