Genotoxicity testing of Persicariae Rhizoma (Persicaria tinctoria H. Gross) aqueous extracts

Lee,Won Ho; Choi,Seong Hun; Kang,Su Jin; Song,Chang Hyun; Park,Soo Jin; Lee,Young Joon; Ku,Sae Kwang

doi:10.3892/etm.2016.3273

Genotoxicity testing of Persicariae Rhizoma (Persicaria tinctoria H. Gross) aqueous extracts

Authors:
- Won Ho Lee
- Seong Hun Choi
- Su Jin Kang
- Chang Hyun Song
- Soo Jin Park
- Young Joon Lee
- Sae Kwang Ku
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Affiliations: Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan, Gyeongbuk 38610, Republic of Korea, Department of Preventive Medicine, College of Korean Medicine, Daegu Haany University, Gyeongsan, Gyeongbuk 38610, Republic of Korea
Published online on: April 20, 2016 https://doi.org/10.3892/etm.2016.3273
Pages: 123-134
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Persicariae Rhizoma (PR) has been used as an anti-inflammatory and detoxification agent in Korea, and contains the biologically active dyes purple indirubin and blue indigo. Despite synthetic indigo showing genotoxic potential, thorough studies have not been carried out on the genotoxicity of PR. The potential genotoxicity of an aqueous extract of PR containing indigo (0.043%) and indirubin (0.009%) was evaluated using a standard battery of tests for safety assessment. The PR extract did not induce any genotoxic effects under the conditions of this study. The results of a reverse mutation assay in four Salmonella typhimurium strains and one Escherichia coli strain indicated that PR extract did not increase the frequency of revertant colonies in any strain, regardless of whether S9 mix was present or not. The PR extract also did not increase chromosomal aberrations in the presence or absence of S9 mix. Although slight signs of diarrhea were restrictedly detected in the mice treated with 2,000 mg/kg PR extract, no noteworthy changes in the frequency of micronucleated polychromatic erythrocytes were observed at doses ≤2,000 mg/kg in a bone marrow micronucleus test. These results indicate the potential safety of the PR extract, particularly if it is consumed in small amounts compared with the quantities used in the genotoxicity tests.

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