Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits

Ji,Mingli; Wang,Yuxia; Wang,Lei; Chen,Liping; Li,Jing

doi:10.3892/etm.2016.3275

Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits

Authors:
- Mingli Ji
- Yuxia Wang
- Lei Wang
- Liping Chen
- Jing Li
View Affiliations

Affiliations: Department of Physiology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Pathophysiology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of General Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Otorhinolaryngology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China
Published online on: April 20, 2016 https://doi.org/10.3892/etm.2016.3275
Pages: 51-58
Copyright: © Ji et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Xuebijing (XBJ) is a Chinese herbal preparation. Previous studies have demonstrated that XBJ injection is able to inhibit the uncontrolled release of endogenous inflammatory mediators, attenuate inflammation, and alleviate organ damage. However, there are no relevant reports on the protective effect of XBJ against left ventricular ischemia/reperfusion (I/R)‑induced acute lung injury (ALI). Therefore, the aim of the present study was to evaluate the protective effect of XBJ on ALI induced by left ventricular I/R, and provide evidence for the clinical application of XBJ. In the present study, 120 healthy rabbits of mixed gender were randomly assigned to a normal control group, ischemia group, I/R group (I/RG) and XBJ‑injection treatment group (TG). In addition, each group was further divided into three subgroups (n=10/subgroup), namely, 30 min pre‑ischemia, 30 min post‑ischemia and 30 min post‑reperfusion subgroups. Blood samples (5 ml) were collected from the jugularis externa and carotis communis of the rabbits at the three time points, and a blood gas analyzer was used to measure the arterial partial pressure of oxygen (PaO2) and carbon dioxide (PaCO2). Following sacrifice, the lungs of the rabbits were removed and a bronchoalveolar lavage (BAL) was immediately performed. An enzyme‑linked immunosorbent assay was used to measure the expression levels of tumor necrosis factor‑α (TNF‑α) in the BAL fluid (BALF) and peripheral blood. In addition, the lower lobe of the right lung was removed in order to measure the protein expression levels of intercellular adhesion molecule‑1 (ICAM‑1) and TNF‑α. The results demonstrated that in the rabbits of the TG PaO2 was increased, PaCO2 was decreased, the lung tissue congestion edema was attenuated, the expression levels of TNF‑α in the peripheral blood and BALF were reduced and the protein expression levels of ICAM‑1 and TNF‑α in the lung tissue samples were decreased, as compared with those in the I/RG rabbits. These results suggest that XBJ may protect against left ventricular I/R‑induced ALI by regulating the expression of the inflammatory mediators TNF‑α and ICAM‑1.

View Figures

View References

1	Waldo SW, Brenner DA, Li S, Alexander K and Ganz P: Reperfusion times and in-hospital outcomes among patients with an isolated posterior myocardial infarction: Insights from the national cardiovascular data registry (NCDR). Am Heart J. 167:350–354. 2014. View Article : Google Scholar : PubMed/NCBI
2	Boersma E, Maas AC, Deckers JW and Simoons ML: Early thrombolytic treatment in acute myocardial infarction: Reappraisal of the golden hour. Lancet. 348:771–775. 1996. View Article : Google Scholar : PubMed/NCBI
3	Buja LM: Myocardial ischemia and reperfusion injury. Cardiovasc Pathol. 14:170–175. 2005. View Article : Google Scholar : PubMed/NCBI
4	Guido BC, Zanatelli M, Tavares-de-Lima W, Oliani SM and Damazo AS: Annexin-A1 peptide down-regulates the leukocyte recruitment and up-regulates interleukin-10 release into lung after intestinal ischemia-reperfusion in mice. J Inflamm (Lond). 10:102013. View Article : Google Scholar : PubMed/NCBI
5	Wang X: Investigational anti-inflammatory agents for the treatment of ischaemic brain injury. Expert Opin Investig Drugs. 14:393–409. 2005. View Article : Google Scholar : PubMed/NCBI
6	Gong P, Lu Z, Xing J, Wang N and Zhang Y: Traditional Chinese medicine Xuebijing treatment is associated with decreased mortality risk of patients with moderate paraquat poisoning. PLoS One. 10:e01235042015. View Article : Google Scholar : PubMed/NCBI
7	Wang YX, Ji ML, Chen LP, Wu XJ and Wang L: Breviscapine reduces acute lung injury induced by left heart ischemic reperfusion in rats by inhibiting the expression of ICAM-1 and IL-18. Exp Ther Med. 6:1322–1326. 2013.PubMed/NCBI
8	Hou SY, Feng XH, Lin CL and Tan YF: Efficacy of Xuebijing for coagulopathy in patients with sepsis. Saudi Med J. 36:164–169. 2015. View Article : Google Scholar : PubMed/NCBI
9	Xu Q, Liu J, Guo X, Tang Y, Zhou G, Liu Y, Huang Q, Geng Y, Liu Z and Su L: Xuebijing injection reduces organ injuries and improves survival by attenuating inflammatory responses and endothelial injury in heatstroke mice. BMC Complement Altern Med. 15:42015. View Article : Google Scholar : PubMed/NCBI
10	National Institutes of Health: Guide for the Care and Use of Laboratory Animals (8th). National Academies Press. Washington (DC): 2011.
11	Yu P, Bu H, Wang H, Zhao GP, Zhang J and Zhou Q: Comparative study on image analysis and manual counting of immunohistochemistry. J Biomed Eng. 20:288–290. 2003.(In Chinese).
12	Zhao Q, Sun C, Xu X, Zhou J, Wu Y, Tian Y, Yuan Z and Liu Z: CD34⁺ cell mobilization and upregulation of myocardial cytokines in a rabbit model of myocardial ischemia. Int J Cardiol. 152:18–23. 2011. View Article : Google Scholar : PubMed/NCBI
13	Huang CH, Tsai SK, Chiang SC, Wang YY, Chih CL, Weng ZC and Lai ST: Brief pressure overload preconditions rabbit myocardium independent of adenosine receptor activation. Ann Thorac Surg. 92:1727–1732. 2011. View Article : Google Scholar : PubMed/NCBI
14	Sun ZH: Coronary CT angiography in coronary artery disease: Correlation between virtual intravascular endoscopic appearances and left bifurcation angulation and coronary plaques. Biomed Res Int. 2013:7320592013. View Article : Google Scholar : PubMed/NCBI
15	Arun Prakash, Kailin R. Mesa, Kevin Wilhelmsen, Fengyun Xu, Jeffrey M. Dodd-o and Judith Hellman: Alveolar Macrophages and Toll-like Receptor 4 Mediate Ventilated Lung Ischemia Reperfusion Injury in Mice.
16	Hughes SF, Hendricks BD, Edwards DR, Bastawrous SS, Roberts GE and Middleton JF: Mild episodes of tourniquet-induced forearm ischaemia-reperfusion injury results in leukocyte activation and changes in inflammatory and coagulation markers. J Inflamm (Lond). 4:122007. View Article : Google Scholar : PubMed/NCBI
17	Ranganathan PV, Jayakumar C, Mohamed R, Dong Z and Ramesh G: Netrin-1 regulates the inflammatory response of neutrophils and macrophages and suppresses ischemic acute kidney injury by inhibiting COX-2 mediated PGE2 production. Kidney Int. 83:1087–1098. 2013. View Article : Google Scholar : PubMed/NCBI
18	Dziodzio T, Biebl M and Pratschke J: Impact of brain death on ischemia/reperfusion injury in liver transplantation. Curr Opin Organ Transplant. 19:108–114. 2014. View Article : Google Scholar : PubMed/NCBI
19	Zygner W, Gójska-Zygner O, Bąska P and Długosz E: Increased concentration of serum TNF alpha and its correlations with arterial blood pressure and indices of renal damage in dogs infected with Babesia canis. Parasitol Res. 113:1499–1503. 2014. View Article : Google Scholar : PubMed/NCBI
20	Lee JW, Kim SC, Ko YS, Lee HY, Cho E, Kim MG, Jo SK, Cho WY and Kim HK: Renoprotective effect of paricalcitol via a modulation of the TLR4-NF-κB pathway in ischemia/reperfusion-induced acute kidney injury. Biochem Biophys Res Commun. 444:121–127. 2014. View Article : Google Scholar : PubMed/NCBI
21	la Guzmán-de Garza FJ, Ibarra-Hernández JM, Cordero-Pérez P, Villegas-Quintero P, Villarreal-Ovalle CI, Torres-González L, Oliva-Sosa NE, Alarcón-Galván G, Fernández-Garza NE, Muñoz-Espinosa LE, et al: Temporal relationship of serum markers and tissue damage during acute intestinal ischemia/reperfusion. Clinics (Sao Paulo). 68:1034–1038. 2013. View Article : Google Scholar : PubMed/NCBI
22	Salim SY, Young PY, Lukowski CM, Madsen KL, Sis B, Churchill TA and Khadaroo RG: VSL#3 probiotics provide protection against acute intestinal ischaemia/reperfusion injury. Benef Microbes. 4:357–365. 2013. View Article : Google Scholar : PubMed/NCBI
23	Pérez-de Puig I, Miró F, Salas-Perdomo A, Bonfill-Teixidor E, Ferrer-Ferrer M, Márquez-Kisinousky L and Planas AM: IL-10 deficiency exacerbates the brain inflammatory response to permanent ischemia without preventing resolution of the lesion. J Cereb Blood Flow Metab. 33:1955–1966. 2013. View Article : Google Scholar : PubMed/NCBI
24	Bodhankar S, Chen Y, Vandenbark AA, Murphy SJ and Offner H: IL-10-producing B-cells limit CNS inflammation and infarct volume in experimental stroke. Metab Brain Dis. 28:375–386. 2013. View Article : Google Scholar : PubMed/NCBI
25	Volin MV and Koch AE: Interleukin-18: A mediator of inflammation and angiogenesis in rheumatoid arthritis. J Interferon Cytokine Res. 31:745–751. 2011. View Article : Google Scholar : PubMed/NCBI
26	Celik E, Faridi MH, Kumar V, Deep S, Moy VT and Gupta V: Agonist leukadherin-1 increases CD11b/CD18-dependent adhesion via membrane tethers. Biophys J. 105:2517–2527. 2013. View Article : Google Scholar : PubMed/NCBI
27	He P, Srikrishna G and Freeze HH: N-glycosylation deficiency reduces ICAM-1 induction and impairs inflammatory response. Glycobiology. 24:392–398. 2014. View Article : Google Scholar : PubMed/NCBI
28	Pillay J, Kamp VM, Pennings M, Oudijk EJ, Leenen LP, Ulfman LH and Koenderman L: Acute-phase concentrations of soluble fibrinogen inhibit neutrophil adhesion under flow conditions in vitro through interactions with ICAM-1 and MAC-1 (CD11b/CD18). J Thromb Haemos. 11:1172–1182. 2013. View Article : Google Scholar
29	Wu R, Zhou Q, Lin S, Ao X, Chen X and Yang J: Effect of cordceps sinensis on the expression of ICAM-1 and VCAM-1 in the kidney of spontaneously hypertensive rats. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 35:152–158. 2010.(In Chinese). PubMed/NCBI
30	Sakai N, Shin T, Schuster R, Blanchard J, Lentsch AB, Johnson WT and Schuschke DA: Marginal copper deficiency increases liver neutrophil accumulation after ischemia/reperfusion in rats. Biol Trace Elem Res. 142:47–54. 2011. View Article : Google Scholar : PubMed/NCBI
31	Liu MW, Wang YH, Qian CY and Li H: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis. Int J Mol Med. 34:1492–1504. 2014.PubMed/NCBI
32	Gong P, Lu Z, Xing J, Wang N and Zhang Y: Traditional Chinese medicine Xuebijing treatment is associated with decreased mortality risk of patients with moderate paraquat poisoning. PLoS One. 10:e01235042015. View Article : Google Scholar : PubMed/NCBI