Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2016 Volume 12 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2016 Volume 12 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Long‑term expression of zinc transporters in hippocampus following penicillin‑induced developmental seizures and its regulation by E‑64d

  • Authors:
    • Hong Ni
    • Le‑Ling Zhang
    • Shou‑Yun Ren
    • Bao‑Liang Sun
  • View Affiliations / Copyright

    Affiliations: Neurology Laboratory, Institute of Pediatrics, Children's Hospital of Soochow University, Suzhou, Jiangsu 215003, P.R. China, Key Laboratory of Cerebral Microcirculation in Universities of Shandong, Department of Neurology, Affiliated Hospital of Taishan Medical College, Taian, Shandong 271000, P.R. China
    Copyright: © Ni et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 208-214
    |
    Published online on: April 20, 2016
       https://doi.org/10.3892/etm.2016.3276
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Autophagy has been shown to be involved in the pathophysiology of developmental seizure‑induced brain damage. The present study aimed to examine whether E‑64d, an autophagy inhibitor, was able to facilitate developmental seizure‑induced hippocampal mossy fiber sprouting, in particular sprouting‑associated zinc transporter signals. Recurrent seizures were induced by penicillin every other day in Sprague‑Dawley rats from postnatal day 21 (P21). Rats were randomly assigned into the control group (CONT), recurrent seizure group (RS) and the seizure plus E‑64d group (E64D). The expression levels of beclin‑1 and B‑cell lymphoma 2 were analyzed at 1.5, 3, 6 and 24 h after the last seizures using western blot analysis. At P51, mossy fiber sprouting and the mRNA expression levels of zinc transporter 2 (ZnT‑2), ZnT‑4, ZnT‑5, ZnT‑6, ZnT‑7, divalent cation transporter 1, Zrt‑Irt‑like protein 6 (ZIP‑6), ZIP‑7, cathepsin D and cathepsin L in the rat hippocampus were assessed using Timm staining and reverse transcription‑quantitative polymerase chain reaction analysis, respectively. Reduced hippocampal mossy fiber sprouting were detected in the E‑64d‑treated rats compared with the non‑treated control. In parallel with these observations, there was a marked reduction in the mRNA expression levels of ZnT‑4 at P51 in the E‑64d‑treated rat hippocampus compared with the non‑treated seizure group. Linear correlation analysis showed significant inter‑relationship among ZIP‑7, ZnT‑4, ZnT‑5, ZnT‑7, cathepsin D and cathepsin L. These results indicate that the ZnT‑4/ZIP‑7/cathepsin signaling pathway serves a crucial function in the neuroprotective effects of E‑64d. Thus, E‑64d may offer a novel strategy for the development of therapeutic interventions for developmental seizure‑induced brain damage.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Au AK, Bayir H, Kochanek PM and Clark RS: Evaluation of autophagy using mouse models of brain injury. Biochim Biophys Acta. 1802:918–923. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Bae N, Ahn T, Chung S, Oh MS, Ko H, Oh H, Park G and Yang HO: The neuroprotective effect of modified Yeoldahanso-tang via autophagy enhancement in models of Parkinson's disease. J Ethnopharmacol. 134:313–322. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Jing CH, Wang L, Liu PP, Wu C, Ruan D and Chen G: Autophagy activation is associated with neuroprotection against apoptosis via a mitochondrial pathway in a rat model of subarachnoid hemorrhage. Neuroscience. 213:144–153. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Holopainen IE: Seizures in the developing brain: Cellular and molecular mechanisms of neuronal damage, neurogenesis and cellular reorganization. Neurochem Int. 52:935–947. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Dulac O, Nabbout R, Plouin P, Chiron C and Scheffer IE: Early seizures: Causal events or predisposition to adult epilepsy? Lancet Neurol. 6:643–651. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Holmes GL: Effects of seizures on brain development: Lessons from the laboratory. Pediatr Neurol. 33:1–11. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Ni H, Jiang YW, Bo T, Wang JM and Wu XR: C-Fos, N-methyl-D-aspartate receptor 2C, GABA-A-alpha 1 immonoreactivity, seizure latency and neuronal injury following single or recurrent neonatal seizures in hippocampus of Wistar rat. Neurosci Lett. 380:149–154. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Ni H, Jiang YW, Tao LY, Cen JN and Wu XR: Effects of penicillin-induced developmental epilepticus on hippocampal regenerative sprouting, related gene expression and cognitive deficits in rats. Toxicol Lett. 188:161–166. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Ni H, Yan JZ, Zhang LL, Feng X and Wu XR: Long-term effects of recurrent neonatal seizures on neurobehavioral function and related gene expression and its intervention by inhibitor of cathepsin B. Neurochem Res. 37:31–39. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Ni H, Feng X, Gong Y, Tao LY and Wu XR: Acute phase expression pattern of ZnTs, LC3 and beclin-1 in rat Hippocampus and its regulation by 3-methyladenine following recurrent neonatal seizures. Biol Trace Elem Res. 143:320–331. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Luo CL, Chen XP, Yang R, Sun YX, Li QQ, Bao HJ, Cao QQ, Ni H, Qin ZH and Tao LY: Cathepsin B contributes to traumatic brain injury-induced cell death through a mitochondria-mediated apoptotic pathway. J Neurosci Res. 88:2847–2858. 2010.PubMed/NCBI

12 

Ni H, Jiang YW, Tao LY, Jin MF and Wu XR: ZnT-1, ZnT-3, CaMKII, PRG-1 expressions in hippocampus following neonatal seizure-induced cognitive deficit in rats. Toxicol Lett. 184:145–150. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Johnson MR, Wang K, Smith JB, Heslin MJ and Diasio RB: Quantitation of dihydropyrimidine dehydrogenase expression by real-time reverse transcription polymerase chain reaction. Anal Biochem. 278:175–184. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Ray SK, Matzelle DC, Wilford GG, Hogan EL and Banik NL: E-64-d prevents both calpain upregulation and apoptosis in the lesion and penumbra following spinal cord injury in rats. Brain Res. 867:80–89. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Ray SK, Matzelle DD, Wilford GG, Hogan EL and Banik NL: Cell death in spinal cord injury (SCI) requires de novo protein synthesis. Calpain inhibitor E-64-d provides neuroprotection in SCI lesion and penumbra. Ann NY Acad Sci. 939:436–449. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Tsubokawa T, Solaroglu I, Yatsushige H, Cahill J, Yata K and Zhang JH: Cathepsin and calpain inhibitor E64d attenuates matrix metalloproteinase-9 activity after focal cerebral ischemia in rats. Stroke. 37:1888–1894. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Tsubokawa T, Yamaguchi-Okada M, Calvert JW, Solaroglu I, Shimamura N, Yata K and Zhang JH: Neurovascular and neuronal protection by E64d after focal cerebral ischemia in rats. J Neurosci Res. 84:832–840. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Dong XX, Wang YR, Qin S, Liang ZQ, Liu BH, Qin ZH and Wang Y: P53 mediates autophagy activation and mitochondria dysfunction in kainic acid-induced excitotoxicity in primary striatal neurons. Neuroscience. 207:52–64. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Ni H, Ren SY, Zhang LL, Sun Q, Tian T and Feng X: Expression profiles of hippocampal regenerative sprouting-related genes and their regulation by E-64d in a developmental rat model of penicillin-induced recurrent epilepticus. Toxicol Lett. 217:162–169. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Takeda A: Zinc signaling in the hippocampus and its relation to pathogenesis of depression. Mol Neurobiol. 44:166–174. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Mitsuya K, Nitta N and Suzuki F: Persistent zinc depletion in the mossy fiber terminals in the intrahippocampal kainate mouse model of mesial temporal lobe epilepsy. Epilepsia. 50:1979–1990. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Nakashima AS and Dyck RH: Zinc and cortical plasticity. Brain Res Rev. 59:347–373. 2009. View Article : Google Scholar : PubMed/NCBI

23 

McCormick NH and Kelleher SL: ZnT4 provides zinc to zinc-dependent proteins in the trans-Golgi network critical for cell function and Zn export in mammary epithelial cells. Am J Physiol Cell Physiol. 303:C291–C297. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Sutula TP and Dudek FE: Unmasking recurrent excitation generated by mossy fiber sprouting in the epileptic dentate gyrus: An emergent property of a complex system. Prog Brain Res. 163:541–563. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Suzuki T, Ishihara K, Migaki H, Matsuura W, Kohda A, Okumura K, Nagao M, Yamaguchi-Iwai Y and Kambe T: Zinc transporters, ZnT5 and ZnT7, are required for the activation of alkaline phosphatases, zinc-requiring enzymes that are glycosylphosphatidylinositol-anchored to the cytoplasmic membrane. J Biol Chem. 280:637–643. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Lichten LA and Cousins RJ: Mammalian zinc transporters: Nutritional and physiologic regulation. Annu Rev Nutr. 29:153–176. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Mims MP and Prchal JT: Divalent metal transporter 1. Hematology. 10:339–345. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Yan G, Zhang Y, Yu J, Yu Y, Zhang F, Zhang Z, Wu A, Yan X, Zhou Y and Wang F: Slc39a7/zip7 plays a critical role in development and zinc homeostasis in zebrafish. PLoS One. 7:e429392012. View Article : Google Scholar : PubMed/NCBI

29 

Taylor KM, Vichova P, Jordan N, Hiscox S, Hendley R and Nicholson RI: ZIP7-mediated intracellular zinc transport contributes to aberrant growth factor signaling in antihormone-resistant breast cancer Cells. Endocrinology. 149:4912–4920. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Hogstrand C, Kille P, Nicholson RI and Taylor KM: Zinc transporters and cancer: A potential role for ZIP7 as a hub for tyrosine kinase activation. Trends Mol Med. 15:101–111. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Luo CL, Li BX, Li QQ, Chen XP, Sun YX, Bao HJ, Dai DK, Shen YW, Xu HF, Ni H, et al: Autophagy is involved in traumatic brain injury-induced cell death and contributes to functional outcome deficits in mice. Neuroscience. 184:54–63. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Chung H, Yoon YH, Hwang JJ, Cho KS, Koh JY and Kim JG: Ethambutol-induced toxicity is mediated by zinc and lysosomal membrane permeabilization in cultured retinal cells. Toxicol Appl Pharmacol. 235:163–170. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ni H, Zhang LL, Ren SY and Sun BL: Long‑term expression of zinc transporters in hippocampus following penicillin‑induced developmental seizures and its regulation by E‑64d. Exp Ther Med 12: 208-214, 2016.
APA
Ni, H., Zhang, L., Ren, S., & Sun, B. (2016). Long‑term expression of zinc transporters in hippocampus following penicillin‑induced developmental seizures and its regulation by E‑64d. Experimental and Therapeutic Medicine, 12, 208-214. https://doi.org/10.3892/etm.2016.3276
MLA
Ni, H., Zhang, L., Ren, S., Sun, B."Long‑term expression of zinc transporters in hippocampus following penicillin‑induced developmental seizures and its regulation by E‑64d". Experimental and Therapeutic Medicine 12.1 (2016): 208-214.
Chicago
Ni, H., Zhang, L., Ren, S., Sun, B."Long‑term expression of zinc transporters in hippocampus following penicillin‑induced developmental seizures and its regulation by E‑64d". Experimental and Therapeutic Medicine 12, no. 1 (2016): 208-214. https://doi.org/10.3892/etm.2016.3276
Copy and paste a formatted citation
x
Spandidos Publications style
Ni H, Zhang LL, Ren SY and Sun BL: Long‑term expression of zinc transporters in hippocampus following penicillin‑induced developmental seizures and its regulation by E‑64d. Exp Ther Med 12: 208-214, 2016.
APA
Ni, H., Zhang, L., Ren, S., & Sun, B. (2016). Long‑term expression of zinc transporters in hippocampus following penicillin‑induced developmental seizures and its regulation by E‑64d. Experimental and Therapeutic Medicine, 12, 208-214. https://doi.org/10.3892/etm.2016.3276
MLA
Ni, H., Zhang, L., Ren, S., Sun, B."Long‑term expression of zinc transporters in hippocampus following penicillin‑induced developmental seizures and its regulation by E‑64d". Experimental and Therapeutic Medicine 12.1 (2016): 208-214.
Chicago
Ni, H., Zhang, L., Ren, S., Sun, B."Long‑term expression of zinc transporters in hippocampus following penicillin‑induced developmental seizures and its regulation by E‑64d". Experimental and Therapeutic Medicine 12, no. 1 (2016): 208-214. https://doi.org/10.3892/etm.2016.3276
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team