Expression of hyperpolarization-activated cyclic nucleotide-gated channel isoforms in a canine model of atrial fibrillation

  • Authors:
    • Wei He
    • Jian Zhang
    • Tianyi Gan
    • Guojun Xu
    • Baopeng Tang
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  • Published online on: April 21, 2016     https://doi.org/10.3892/etm.2016.3290
  • Pages: 433-436
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Abstract

The aim of the present study was to analyze the mRNA and protein expression levels of atrial hyperpolarization-activated cyclic nucleotide-gated (HCN) channel isoforms in the left atrial muscle of dogs with multiple organ failure. A total of 14 beagle dogs with multiple organ failure, including seven cases with sinus rhythm and seven cases with atrial fibrillation (AF), underwent surgery to remove a sample of left atrial appendage tissue. The expression levels of a number of HCN channel subtypes were subsequently measured using quantitative polymerase chain reaction and western blot analysis. The mRNA and protein expression levels of HCN2 and HCN4 increased significantly in the AF group when compared with the sinus rhythm group. However, expression of the HCN1 isoform was not detected. Therefore, increased expression levels of HCN2 and HCN4 may be important molecular mechanisms in the pathogenesis of AF, which were associated with differences in patients with valvular heart disease.
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July-2016
Volume 12 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
He W, Zhang J, Gan T, Xu G and Tang B: Expression of hyperpolarization-activated cyclic nucleotide-gated channel isoforms in a canine model of atrial fibrillation. Exp Ther Med 12: 433-436, 2016
APA
He, W., Zhang, J., Gan, T., Xu, G., & Tang, B. (2016). Expression of hyperpolarization-activated cyclic nucleotide-gated channel isoforms in a canine model of atrial fibrillation. Experimental and Therapeutic Medicine, 12, 433-436. https://doi.org/10.3892/etm.2016.3290
MLA
He, W., Zhang, J., Gan, T., Xu, G., Tang, B."Expression of hyperpolarization-activated cyclic nucleotide-gated channel isoforms in a canine model of atrial fibrillation". Experimental and Therapeutic Medicine 12.1 (2016): 433-436.
Chicago
He, W., Zhang, J., Gan, T., Xu, G., Tang, B."Expression of hyperpolarization-activated cyclic nucleotide-gated channel isoforms in a canine model of atrial fibrillation". Experimental and Therapeutic Medicine 12, no. 1 (2016): 433-436. https://doi.org/10.3892/etm.2016.3290