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Article

Detection and genotype analysis of AmpC β‑lactamase in Klebsiella pneumoniae from tertiary hospitals

  • Authors:
    • Xiang‑Qun Liu
    • Yong‑Rui Liu
  • View Affiliations / Copyright

    Affiliations: Department of Pneumology, Xuzhou City Hospital Affiliated to Xuzhou Medical College, Xuzhou, Jiangsu 221002, P.R. China
  • Pages: 480-484
    |
    Published online on: April 27, 2016
       https://doi.org/10.3892/etm.2016.3295
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Abstract

The aim of the present study was to investigate the phenotype and genotype of plasmid‑mediated AmpC (pAmpC) β‑lactamase in Klebsiella pneumoniae and its antibiotic resistance. A total of 130 non‑repetitive clinical isolates of Klebsiella pneumoniae, obtained from tertiary hospitals, were phenotypically screened for pAmpC β‑lactamase production with the cefoxitin disk diffusion test. β‑lactamase genes in the screened isolates were detected using multiplex polymerase chain reaction (PCR); carbapenemase genes in pAmpC β‑lactamase‑producing isolates that were resistant to imipenem were detected using PCR. Out of the 130 isolates of Klebsiella pneumoniae, 62 strains (47.7%) were resistant to cefoxitin, including 14 strains (10.8%) positive for pAmpC β‑lactamase (DHA type), among which 12 strains (85.7%) were susceptible to imipenem, and 2 strains, which were carrying Klebsiella pneumoniae carbapenemase (KPC)‑2 gene, were resistant to imipenem. The pAmpC β‑lactamase‑producing Klebsiella pneumoniae isolates from the tertiary hospitals were mainly of DHA‑1 genotype, and the majority were susceptible to carbapenems; drug‑resistant strains were associated with KPC‑2 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Liu XQ and Liu YR: Detection and genotype analysis of AmpC β‑lactamase in Klebsiella pneumoniae from tertiary hospitals. Exp Ther Med 12: 480-484, 2016.
APA
Liu, X., & Liu, Y. (2016). Detection and genotype analysis of AmpC β‑lactamase in Klebsiella pneumoniae from tertiary hospitals. Experimental and Therapeutic Medicine, 12, 480-484. https://doi.org/10.3892/etm.2016.3295
MLA
Liu, X., Liu, Y."Detection and genotype analysis of AmpC β‑lactamase in Klebsiella pneumoniae from tertiary hospitals". Experimental and Therapeutic Medicine 12.1 (2016): 480-484.
Chicago
Liu, X., Liu, Y."Detection and genotype analysis of AmpC β‑lactamase in Klebsiella pneumoniae from tertiary hospitals". Experimental and Therapeutic Medicine 12, no. 1 (2016): 480-484. https://doi.org/10.3892/etm.2016.3295
Copy and paste a formatted citation
x
Spandidos Publications style
Liu XQ and Liu YR: Detection and genotype analysis of AmpC β‑lactamase in Klebsiella pneumoniae from tertiary hospitals. Exp Ther Med 12: 480-484, 2016.
APA
Liu, X., & Liu, Y. (2016). Detection and genotype analysis of AmpC β‑lactamase in Klebsiella pneumoniae from tertiary hospitals. Experimental and Therapeutic Medicine, 12, 480-484. https://doi.org/10.3892/etm.2016.3295
MLA
Liu, X., Liu, Y."Detection and genotype analysis of AmpC β‑lactamase in Klebsiella pneumoniae from tertiary hospitals". Experimental and Therapeutic Medicine 12.1 (2016): 480-484.
Chicago
Liu, X., Liu, Y."Detection and genotype analysis of AmpC β‑lactamase in Klebsiella pneumoniae from tertiary hospitals". Experimental and Therapeutic Medicine 12, no. 1 (2016): 480-484. https://doi.org/10.3892/etm.2016.3295
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