Increased multi‑drug resistance and reduced apoptosis in osteosarcoma side population cells are crucial factors for tumor recurrence

Wang,Yang; Teng,Jia‑Song

doi:10.3892/etm.2016.3303

Increased multi‑drug resistance and reduced apoptosis in osteosarcoma side population cells are crucial factors for tumor recurrence

Authors:
- Yang Wang
- Jia‑Song Teng
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Affiliations: Department of Orthopaedics, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Orthopaedics, Daqing Oilfields General Hospital, Daqing, Heilongjiang 163000, P.R. China
Published online on: April 27, 2016 https://doi.org/10.3892/etm.2016.3303
Pages: 81-86
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the characteristic features of cancer stem cells (CSCs) using an aggressive human osteosarcoma cell line OS‑65. Hoechst 33342 dye exclusion was used to distinguish the cancer stem‑like side population (SP) cells from OS‑65 cells. Furthermore, the SP cells were characterized via chemoresistance and cell death assays, reverse transcription‑quantitative polymerase chain reaction and immunofluorescence. The present study identified ~3.3% of cancer stem‑like SP cells from OS‑65 cells whose prevalence is reduced significantly (0.9%) following treatment with verapamil. It was demonstrated that osteosarcoma SP cells are highly efficient at generating additional sarcospheres as transcriptional regulation of stemness genes, including SOX2, OCT‑4 and NANOG, is highly upregulated. Notably, these SP cells demonstrated high resistance against chemotherapeutic drugs and apoptosis via elevated transcriptional regulation of several ATPase binding cassette (ABC) transporter and anti‑apoptotic proteins, including ABCG2, ABCB1/MDR1 ABCB5, B cell lymphoma‑2 (Bcl‑2) and Bcl‑2 associated X protein, respectively. The results of the present study suggested that CSCs may be a novel therapeutic target for the prevention of tumor relapse.

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