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Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle

  • Authors:
    • Chun Hou
    • Yong Miao
    • Xue Wang
    • Chaoyue Chen
    • Bojie Lin
    • Zhiqi Hu
  • View Affiliations / Copyright

    Affiliations: Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
    Copyright: © Hou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 231-237
    |
    Published online on: May 9, 2016
       https://doi.org/10.3892/etm.2016.3319
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Abstract

According to the growth state of hair follicles, the hair cycle is divided into the anagen, catagen and telogen phases. A number of biological factors have been shown to synchronize with the hair cycle. As an important component of the hair follicle, the extracellular matrix is regulated by matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitor of matrix metalloproteinases; TIMPs). It has been reported that MMP‑2, MMP‑9 and TIMP‑1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated. Reverse transcription‑polymerase chain reaction and ELISA analysis in the present study demonstrated that, during the hair cycle in mice, mRNA and protein expression levels of MMP‑2 and MMP‑9 were elevated in the anagen phase, and decreased during the catagen and telogen phases. Furthermore, SDS‑PAGE gelatin zymography demonstrated that their activities fluctuated in the hair cycle. Additionally, it was observed that the mRNA and protein expression levels of TIMP‑1 and TIMP-2 were negatively correlated with MMP‑9 and MMP‑2, respectively. Immunohistochemical examination demonstrated that MMP-2 and TIMP-2 were present in all structures of the hair follicle. However, MMP‑9 and TIMP‑1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase. These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.
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Copy and paste a formatted citation
Spandidos Publications style
Hou C, Miao Y, Wang X, Chen C, Lin B and Hu Z: Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle. Exp Ther Med 12: 231-237, 2016.
APA
Hou, C., Miao, Y., Wang, X., Chen, C., Lin, B., & Hu, Z. (2016). Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle. Experimental and Therapeutic Medicine, 12, 231-237. https://doi.org/10.3892/etm.2016.3319
MLA
Hou, C., Miao, Y., Wang, X., Chen, C., Lin, B., Hu, Z."Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle". Experimental and Therapeutic Medicine 12.1 (2016): 231-237.
Chicago
Hou, C., Miao, Y., Wang, X., Chen, C., Lin, B., Hu, Z."Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle". Experimental and Therapeutic Medicine 12, no. 1 (2016): 231-237. https://doi.org/10.3892/etm.2016.3319
Copy and paste a formatted citation
x
Spandidos Publications style
Hou C, Miao Y, Wang X, Chen C, Lin B and Hu Z: Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle. Exp Ther Med 12: 231-237, 2016.
APA
Hou, C., Miao, Y., Wang, X., Chen, C., Lin, B., & Hu, Z. (2016). Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle. Experimental and Therapeutic Medicine, 12, 231-237. https://doi.org/10.3892/etm.2016.3319
MLA
Hou, C., Miao, Y., Wang, X., Chen, C., Lin, B., Hu, Z."Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle". Experimental and Therapeutic Medicine 12.1 (2016): 231-237.
Chicago
Hou, C., Miao, Y., Wang, X., Chen, C., Lin, B., Hu, Z."Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle". Experimental and Therapeutic Medicine 12, no. 1 (2016): 231-237. https://doi.org/10.3892/etm.2016.3319
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