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Article Open Access

Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid

  • Authors:
    • Ji Young Lee
    • Wansu Park
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, College of Korean Medicine, Gachon University, Seongnam, Gyeonggi 461‑701, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 151-156
    |
    Published online on: May 9, 2016
       https://doi.org/10.3892/etm.2016.3320
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Abstract

Oroxylin A (5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one; Baicalein 6-methyl ether) is an active flavonoid compound originally isolated from Scutellaria radix, which has been used to treat pulmonary infection in Korea, China, and Japan. Oroxylin A is known to possess dopamine reuptake inhibitor activity. However, the effects of oroxylin A on virus‑induced macrophages has not been fully elucidated. In the present study, the anti‑inflammatory effects of oroxylin A on double‑stranded RNA‑induced macrophages were examined. Production of nitric oxide (NO), various cytokines, as well as calcium release and the mRNA expression of signal transducer and activator of transcription 1 (STAT1) in dsRNA polyinosinic-polycytidylic acid (PIC)‑induced RAW 264.7 mouse macrophages were evaluated. Oroxylin A restored the cell viability in PIC‑induced RAW 264.7 mouse macrophages at concentrations of up to 50 µM. Additionally, oroxylin A significantly inhibited the production of nitric oxide (NO), interleukin (IL)‑1α, IL‑1β, IL‑6, IL‑10, interferon gamma‑induced protein 10, granulocyte‑colony stimulating factor (CSF), granulocyte macrophage‑CSF, leukemia inhibitory factor (IL‑6 class cytokine), lipopolysaccharide‑induced CXC chemokine (LIX), monocyte chemoattractant protein 1, macrophage inflammatory protein (MIP)‑1α, MIP‑1β, MIP‑2, Regulated on Activation, Normal T Expressed and Secreted, tumor necrosis factor‑α, and vascular endothelial growth factor as well as calcium release and the mRNA expression of STAT1 in PIC‑induced RAW 264.7 cells (P<0.05). Thus, the present results suggest that oroxylin A has anti‑inflammatory properties, associated with its inhibition of NO, cytokines, chemokines and growth factors in PIC-induced macrophages via the calcium-STAT pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Lee JY and Park W: Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid. Exp Ther Med 12: 151-156, 2016.
APA
Lee, J.Y., & Park, W. (2016). Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid. Experimental and Therapeutic Medicine, 12, 151-156. https://doi.org/10.3892/etm.2016.3320
MLA
Lee, J. Y., Park, W."Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid". Experimental and Therapeutic Medicine 12.1 (2016): 151-156.
Chicago
Lee, J. Y., Park, W."Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid". Experimental and Therapeutic Medicine 12, no. 1 (2016): 151-156. https://doi.org/10.3892/etm.2016.3320
Copy and paste a formatted citation
x
Spandidos Publications style
Lee JY and Park W: Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid. Exp Ther Med 12: 151-156, 2016.
APA
Lee, J.Y., & Park, W. (2016). Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid. Experimental and Therapeutic Medicine, 12, 151-156. https://doi.org/10.3892/etm.2016.3320
MLA
Lee, J. Y., Park, W."Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid". Experimental and Therapeutic Medicine 12.1 (2016): 151-156.
Chicago
Lee, J. Y., Park, W."Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid". Experimental and Therapeutic Medicine 12, no. 1 (2016): 151-156. https://doi.org/10.3892/etm.2016.3320
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