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Nrdp1-mediated degradation of BRUCE decreases cell viability and induces apoptosis in human 786-O renal cell carcinoma cells

  • Authors:
    • Shao‑Jun Chen
    • Jian‑Hai Lin
    • Xu‑Dong Yao
    • Bo Peng
    • Yun‑Fei Xu
    • Min Liu
    • Jun‑Hua Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 597-602
    |
    Published online on: May 18, 2016
       https://doi.org/10.3892/etm.2016.3356
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Abstract

Neuregulin receptor degradation protein-1 (Nrdp1) is involved in a plethora of cellular processes and plays an essential role in the development and progression of human cancers. However, its role in renal cell carcinoma (RCC) remains unclear. Therefore, the present study aimed to explore the biological significance of Nrdp1 in RCC. Western blot analyses of tissue samples from 24 patients with primary RCC revealed lower Nrdp1 and higher baculovirus inhibitor of apoptosis repeat‑containing ubiquitin‑conjugating enzyme (BRUCE) protein levels in RCC tissues compared with adjacent normal tissues. In addition, MTT and apoptosis assays demonstrated that Nrdp1 overexpression resulted in decreased cell viability and enhanced apoptosis in RCC 786‑O cells; conversely, Nrdp1 knockdown increased 786‑O cell viability and inhibited apoptosis. Further analysis showed that BRUCE downregulation partially attenuated the effects of Nrdp1 knockdown on RCC cell viability and apoptosis. Moreover, an inverse association was obtained between BRUCE and Nrdp1 protein levels. These findings suggest that Nrdp1‑mediated degradation of BRUCE decreases cell viability and induces apoptosis in RCC cells, highlighting Nrdp1 as a potential target for RCC treatment.
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1 

Yang J, Yang J, Gao Y, Zhao L, Liu L, Qin Y, Wang X, Song T and Huang C: Identification of potential serum proteomic biomarkers for clear cell renal cell carcinoma. PLoS One. 9:e1113642014. View Article : Google Scholar : PubMed/NCBI

2 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Lasseigne BN, Burwell TC, Patil MA, Absher DM, Brooks JD and Myers RM: DNA methylation profiling reveals novel diagnostic biomarkers in renal cell carcinoma. BMC Med. 12:2352014. View Article : Google Scholar : PubMed/NCBI

4 

Janowitz T, Welsh SJ, Zaki K, Mulders P and Eisen T: Adjuvant therapy in renal cell carcinoma - past, present, and future. Semin Oncol. 40:482–491. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Li W, Liu M, Xu YF, Feng Y, Che JP, Wang GC and Zheng JH: Combination of quercetin and hyperoside has anticancer effects on renal cancer cells through inhibition of oncogenic microRNA-27a. Oncol Rep. 31:117–124. 2014.PubMed/NCBI

6 

Zhang HM, Yang FQ, Yan Y, Che JP and Zheng JH: High expression of long non-coding RNA SPRY4-IT1 predicts poor prognosis of clear cell renal cell carcinoma. Int J Clin Exp Pathol. 7:5801–5809. 2014.PubMed/NCBI

7 

Bouyain S and Leahy DJ: Structure-based mutagenesis of the substrate-recognition domain of Nrdp1/FLRF identifies the binding site for the receptor tyrosine kinase ErbB3. Protein Sci. 16:654–661. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Meroni G and Diez-Roux G: TRIM/RBCC, a novel class of ‘single protein RING finger’ E3 ubiquitin ligases. Bioessays. 27:1147–1157. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Qiu XB and Goldberg AL: Nrdp1/FLRF is a ubiquitin ligase promoting ubiquitination and degradation of the epidermal growth factor receptor family member, ErbB3. Proc Natl Acad Sci USA. 99:14843–14848. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Yen L, Cao Z, Wu X, Ingalla ER, Baron C, Young LJ, Gregg JP, Cardiff RD, Borowsky AD, Sweeney C and Carraway KL III: Loss of Nrdp1 enhances ErbB2/ErbB3-dependent breast tumor cell growth. Cancer Res. 66:11279–11286. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Lu H, Li H, Mao D, Zhu Z and Sun H: Nrdp1 inhibits growth of colorectal cancer cells by nuclear retention of p27. Tumour Biol. 35:8639–8643. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Qiu XB, Markant SL, Yuan J and Goldberg AL: Nrdp1-mediated degradation of the gigantic IAP, BRUCE, is a novel pathway for triggering apoptosis. EMBO J. 23:800–810. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Yu F and Zhou J: Parkin is ubiquitinated by Nrdp1 and abrogates Nrdp1-induced oxidative stress. Neurosci Lett. 440:4–8. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Wang C, Chen T, Zhang J, Yang M, Li N, Xu X and Cao X: The E3 ubiquitin ligase Nrdp1 ‘preferentially’ promotes TLR-mediated production of type I interferon. Nat Immunol. 10:744–752. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Chen Z, Naito M, Hori S, Mashima T, Yamori T and Tsuruo T: A human IAP-family gene, apollon, expressed in human brain cancer cells. Biochem Biophys Res Commun. 264:847–854. 1999. View Article : Google Scholar : PubMed/NCBI

16 

Bartke T, Pohl C, Pyrowolakis G and Jentsch S: Dual role of BRUCE as an antiapoptotic IAP and a chimeric E2/E3 ubiquitin ligase. Mol Cell. 14:801–811. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Qiu XB and Goldberg AL: The membrane-associated inhibitor of apoptosis protein, BRUCE/Apollon, antagonizes both the precursor and mature forms of Smac and caspase-9. J Biol Chem. 280:174–182. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Hao Y, Sekine K, Kawabata A, Nakamura H, Ishioka T, Ohata H, Katayama R, Hashimoto C, Zhang X, Noda T, et al: Apollon ubiquitinates SMAC and caspase-9, and has an essential cytoprotection function. Nat Cell Biol. 6:849–860. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Shi H, Du J, Wang L, Zheng B, Gong H, Wu Y, Tang Y, Gao Y and Yu R: Lower expression of Nrdp1 in human glioma contributes tumor progression by reducing apoptosis. IUBMB Life. 66:704–710. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Valsechi MC, Oliveira AB, Conceição AL, Stuqui B, Candido NM, Provazzi PJ, de Araújo LF, Silva WA Jr, Calmon Mde F and Rahal P: GPC3 reduces cell proliferation in renal carcinoma cell lines. BMC Cancer. 14:6312014. View Article : Google Scholar : PubMed/NCBI

22 

Qiao HP, Gao WS, Huo JX and Yang ZS: Long non-coding RNA GAS5 functions as a tumor suppressor in renal cell carcinoma. Asian Pac J Cancer Prev. 14:1077–1082. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Zhang T, Zheng J, Jiang N, Wang G, Shi Q, Liu C and Lu Y: Overexpression of DLC-1 induces cell apoptosis and proliferation inhibition in the renal cell carcinoma. Cancer Lett. 283:59–67. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Hu G, Lai P, Liu M, Xu L, Guo Z, Liu H, Li W, Wang G, Yao X, Zheng J and Xu Y: MiR-203a regulates proliferation, migration, and apoptosis by targeting glycogen synthase kinase-3β in human renal cell carcinoma. Tumour Biol. 35:11443–11453. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Hassan M, Watari H, AbuAlmaaty A, Ohba Y and Sakuragi N: Apoptosis and molecular targeting therapy in cancer. Biomed Res Int. 2014:1508452014. View Article : Google Scholar : PubMed/NCBI

26 

Wong RS: Apoptosis in cancer: From pathogenesis to treatment. J Exp Clin Cancer Res. 30:872011. View Article : Google Scholar : PubMed/NCBI

27 

Bartke T, Pohl C, Pyrowolakis G and Jentsch S: Dual role of BRUCE as an antiapoptotic IAP and a chimeric E2/E3 ubiquitin ligase. Mol Cell. 14:801–811. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Ren J, Shi M, Liu R, Yang QH, Johnson T, Skarnes WC and Du C: The Birc6 (Bruce) gene regulates p53 and the mitochondrial pathway of apoptosis and is essential for mouse embryonic development. Proc Natl Acad Sci USA. 102:565–570. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen SJ, Lin JH, Yao XD, Peng B, Xu YF, Liu M and Zheng JH: Nrdp1-mediated degradation of BRUCE decreases cell viability and induces apoptosis in human 786-O renal cell carcinoma cells. Exp Ther Med 12: 597-602, 2016.
APA
Chen, S., Lin, J., Yao, X., Peng, B., Xu, Y., Liu, M., & Zheng, J. (2016). Nrdp1-mediated degradation of BRUCE decreases cell viability and induces apoptosis in human 786-O renal cell carcinoma cells. Experimental and Therapeutic Medicine, 12, 597-602. https://doi.org/10.3892/etm.2016.3356
MLA
Chen, S., Lin, J., Yao, X., Peng, B., Xu, Y., Liu, M., Zheng, J."Nrdp1-mediated degradation of BRUCE decreases cell viability and induces apoptosis in human 786-O renal cell carcinoma cells". Experimental and Therapeutic Medicine 12.2 (2016): 597-602.
Chicago
Chen, S., Lin, J., Yao, X., Peng, B., Xu, Y., Liu, M., Zheng, J."Nrdp1-mediated degradation of BRUCE decreases cell viability and induces apoptosis in human 786-O renal cell carcinoma cells". Experimental and Therapeutic Medicine 12, no. 2 (2016): 597-602. https://doi.org/10.3892/etm.2016.3356
Copy and paste a formatted citation
x
Spandidos Publications style
Chen SJ, Lin JH, Yao XD, Peng B, Xu YF, Liu M and Zheng JH: Nrdp1-mediated degradation of BRUCE decreases cell viability and induces apoptosis in human 786-O renal cell carcinoma cells. Exp Ther Med 12: 597-602, 2016.
APA
Chen, S., Lin, J., Yao, X., Peng, B., Xu, Y., Liu, M., & Zheng, J. (2016). Nrdp1-mediated degradation of BRUCE decreases cell viability and induces apoptosis in human 786-O renal cell carcinoma cells. Experimental and Therapeutic Medicine, 12, 597-602. https://doi.org/10.3892/etm.2016.3356
MLA
Chen, S., Lin, J., Yao, X., Peng, B., Xu, Y., Liu, M., Zheng, J."Nrdp1-mediated degradation of BRUCE decreases cell viability and induces apoptosis in human 786-O renal cell carcinoma cells". Experimental and Therapeutic Medicine 12.2 (2016): 597-602.
Chicago
Chen, S., Lin, J., Yao, X., Peng, B., Xu, Y., Liu, M., Zheng, J."Nrdp1-mediated degradation of BRUCE decreases cell viability and induces apoptosis in human 786-O renal cell carcinoma cells". Experimental and Therapeutic Medicine 12, no. 2 (2016): 597-602. https://doi.org/10.3892/etm.2016.3356
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