Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2016 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2016 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

MicroRNA-135b has a neuroprotective role via targeting of β-site APP-cleaving enzyme 1

  • Authors:
    • Yi Zhang
    • Hongxia Xing
    • Shuangxi Guo
    • Zhiyong Zheng
    • Haoliang Wang
    • Dawei Xu
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Neurosurgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China
  • Pages: 809-814
    |
    Published online on: May 19, 2016
       https://doi.org/10.3892/etm.2016.3366
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

MicroRNAs (miRs) are a class of endogenous small non-coding RNAs that have been revealed to negatively mediate the expression of their target genes at the post-transcriptional level. Recently, particular miRs have demonstrated an involvement in the pathogenesis of Alzheimer's disease (AD). However, the specific role of miR‑135b in AD has yet to be elucidated. The present study aimed to investigate the neuroprotective role of miR‑135b, in addition to its underlying mechanism. Herein, reverse transcription‑quantitative polymerase chain reaction was conducted to determine miR‑135b expression levels in the peripheral blood samples of patients with AD and age‑matched normal controls. The data of the present study revealed that the expression levels of miR‑135b were significantly reduced in the peripheral blood of AD patients compared with normal controls (P<0.01). In vitro MTT analyses identified that the overexpression of miR-135b significantly enhanced the proliferation of hippocampal cells (P<0.01). Furthermore, in vivo analysis using a Y‑maze test indicated that injection with miR‑135b mimics into the third ventricle of anesthetized SAMP8 mice significantly enhanced their learning and memory capacities (P<0.01). Molecular mechanism investigations identified β‑site APP‑cleaving enzyme 1 (BACE1) as a direct target gene of miR‑135b, and the latter was identified to negatively mediate the protein expression levels of BACE1 in hippocampal cells, in addition to hippocampal tissues, of SAMP8 mice. Based on the aforementioned findings, we propose that miR‑135b has a neuroprotective role via direct targeting of BACE1 and, thus, may be used for the treatment of AD.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Zlomuzica A, Dere D, Binder S, De Souza Silva MA, Huston JP and Dere E: Neuronal histamine and cognitive symptoms in Alzheimer's disease. Neuropharmacology. May 27–2015.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

2 

Dezsi L, Tuka B, Martos D and Vecsei L: Alzheimer's disease, astrocytes and kynurenines. Curr Alzheimer Res. 12:462–480. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Alzheimer's Association: 2015 Alzheimer's disease facts and figures. Alzheimers Dement. 11:332–384. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Lei X, Lei L, Zhang Z, Zhang Z and Cheng Y: Downregulated miR-29c correlates with increased BACE1 expression in sporadic Alzheimer's disease. Int J Clin Exp Pathol. 8:1565–1574. 2015.PubMed/NCBI

5 

Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Yang G, Song Y, Zhou X, Deng Y, Liu T, Weng G, Yu D and Pan S: DNA methyltransferase 3, a target of microRNA-29c, contributes to neuronal proliferation by regulating the expression of brain-derived neurotrophic factor. Mol Med Rep. 12:1435–1442. 2015.PubMed/NCBI

7 

Stary CM, Xu L, Sun X, Ouyang YB, White RE, Leong J, Li J, Xiong X and Giffard RG: MicroRNA-200c contributes to injury from transient focal cerebral ischemia by targeting Reelin. Stroke. 46:551–556. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Denk J, Boelmans K, Siegismund C, Lassner D, Arlt S and Jahn H: MicroRNA Profiling of CSF reveals potential biomarkers to detect Alzheimer's disease. PLoS One. 10:e01264232015. View Article : Google Scholar : PubMed/NCBI

9 

Yang G, Song Y, Zhou X, Deng Y, Liu T, Weng G, Yu D and Pan S: MicroRNA-29c targets β-site amyloid precursor protein-cleaving enzyme 1 and has a neuroprotective role in vitro and in vivo. Mol Med Rep. 2015. View Article : Google Scholar

10 

Zhu Y, Li C, Sun A, Wang Y and Zhou S: Quantification of microRNA-210 in the cerebrospinal fluid and serum: Implications for Alzheimer's disease. Exp Ther Med. 9:1013–1017. 2015.PubMed/NCBI

11 

Liu CG, Wang JL, Li L, Xue LX, Zhang YQ and Wang PC: MicroRNA-135a and −200b, potential Biomarkers for Alzheimer's disease, regulate β secretase and amyloid precursor protein. Brain Res. 1583:55–64. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals: Washington (DC) (8th). National Academies Press. US: 2011.

14 

Hansen HH, Fabricius K, Barkholt P, Niehoff ML, Morley JE, Jelsing J, Pyke C, Knudsen LB, Farr SA and Vrang N: The GLP-1 receptor agonist liraglutide improves memory function and increases hippocampal CA1 neuronal numbers in a senescence-accelerated mouse model of Alzheimer's disease. J Alzheimers Dis. 46:877–888. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Takagane K, Nojima J, Mitsuhashi H, Suo S, Yanagihara D, Takaiwa F, Urano Y, Noguchi N and Ishiura S: Aβ induces oxidative stress in senescence-accelerated (SAMP8) mice. Biosci Biotechnol Biochem. 79:912–918. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Decourt B, Walker A, Gonzales A, Malek-Ahmadi M, Liesback C, Davis KJ, Belden CM, Jacobson SA and Sabbagh MN: Can platelet BACE1 levels be used as a biomarker for Alzheimer's disease? Proof-of-concept study. Platelets. 24:235–238. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Shaw LM, Vanderstichele H, Knapik-Czajka M, Clark CM, Aisen PS, Petersen RC, Blennow K, Soares H, Simon A, Lewczuk P, et al: Alzheimer's Disease Neuroimaging Initiative: Cerebrospinal fluid biomarker signature in Alzheimer's disease neuroimaging initiative subjects. Ann Neurol. 65:403–413. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Bibl M, Esselmann H, Lewczuk P, Trenkwalder CF, Otto M, Kornhuber J, Wiltfang J and Mollenhauer B: Combined analysis of CSF Tau, Aβ42, Aβ1–42% and Aβ1–40% in Alzheimer's disease, dementia with Lewy bodies and Parkinson's disease dementia. Int J Alzheimers Dis 2010. Article ID 761571. 2010.

19 

Hébert SS, Horré K, Nicolaï L, Papadopoulou AS, Mandemakers W, Silahtaroglu AN, Kauppinen S, Delacourte A and De Strooper B: Loss of microRNA cluster miR-29a/b-1 in sporadic Alzheimer's disease correlates with increased BACE1/beta-secretase expression. Proc Natl Acad Sci USA. 105:6415–6420. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Müller M, Kuiperij HB, Claassen JA, Küsters B and Verbeek MM: MicroRNAs in Alzheimer's disease: Differential expression in hippocampus and cell-free cerebrospinal fluid. Neurobiol Aging. 35:152–158. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Moon M, Cha MY and Mook-Jung I: Impaired hippocampal neurogenesis and its enhancement with ghrelin in 5XFAD mice. J Alzheimers Dis. 41:233–241. 2014.PubMed/NCBI

22 

Cui Y, Xiao Z, Han J, Sun J, Ding W, Zhao Y, Chen B, Li X and Dai J: MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin. BMC Neurosci. 13:1162012. View Article : Google Scholar : PubMed/NCBI

23 

Mollinari C, Racaniello M, Berry A, Pieri M, de Stefano MC, Cardinale A, Zona C, Cirulli F, Garaci E and Merlo D: miR-34a regulates cell proliferation, morphology and function of newborn neurons resulting in improved behavioural outcomes. Cell Death Dis. 6:e16222015. View Article : Google Scholar : PubMed/NCBI

24 

Buckley RF, Ellis KA, Ames D, Rowe CC, Lautenschlager NT, Maruff P, Villemagne VL, Macaulay SL, Szoeke C, Martins RN, et al: Phenomenological characterization of memory complaints in preclinical and prodromal Alzheimer's disease. Neuropsychology. 29:571–581. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Vassar R, Kuhn PH, Haass C, Kennedy ME, Rajendran L, Wong PC and Lichtenthaler SF: Function, therapeutic potential and cell biology of BACE proteases: Current status and future prospects. J Neurochem. 130:4–28. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Yan R and Vassar R: Targeting the β secretase BACE1 for Alzheimer's disease therapy. Lancet Neurol. 13:319–329. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Long JM, Ray B and Lahiri DK: MicroRNA-339-5p down-regulates protein expression of β-site amyloid precursor protein-cleaving enzyme 1 (BACE1) in human primary brain cultures and is reduced in brain tissue specimens of Alzheimer disease subjects. J Biol Chem. 289:5184–5198. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Zhu HC, Wang LM, Wang M, Song B, Tan S, Teng JF and Duan DX: MicroRNA-195 downregulates Alzheimer's disease amyloid-beta production by targeting BACE1. Brain Res Bull. 88:596–601. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Roshan R, Ghosh T, Gadgil M and Pillai B: Regulation of BACE1 by miR-29a/b in a cellular model of Spinocerebellar Ataxia 17. RNA Biol. 9:891–899. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Fang M, Wang J, Zhang X, Geng Y, Hu Z, Rudd JA, Ling S, Chen W and Han S: The miR-124 regulates the expression of BACE1/β-secretase correlated with cell death in Alzheimer's disease. Toxicol Lett. 209:94–105. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Xing H, Guo S, Zheng Z, Wang H and Xu D: MicroRNA-135b has a neuroprotective role via targeting of β-site APP-cleaving enzyme 1. Exp Ther Med 12: 809-814, 2016.
APA
Zhang, Y., Xing, H., Guo, S., Zheng, Z., Wang, H., & Xu, D. (2016). MicroRNA-135b has a neuroprotective role via targeting of β-site APP-cleaving enzyme 1. Experimental and Therapeutic Medicine, 12, 809-814. https://doi.org/10.3892/etm.2016.3366
MLA
Zhang, Y., Xing, H., Guo, S., Zheng, Z., Wang, H., Xu, D."MicroRNA-135b has a neuroprotective role via targeting of β-site APP-cleaving enzyme 1". Experimental and Therapeutic Medicine 12.2 (2016): 809-814.
Chicago
Zhang, Y., Xing, H., Guo, S., Zheng, Z., Wang, H., Xu, D."MicroRNA-135b has a neuroprotective role via targeting of β-site APP-cleaving enzyme 1". Experimental and Therapeutic Medicine 12, no. 2 (2016): 809-814. https://doi.org/10.3892/etm.2016.3366
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Xing H, Guo S, Zheng Z, Wang H and Xu D: MicroRNA-135b has a neuroprotective role via targeting of β-site APP-cleaving enzyme 1. Exp Ther Med 12: 809-814, 2016.
APA
Zhang, Y., Xing, H., Guo, S., Zheng, Z., Wang, H., & Xu, D. (2016). MicroRNA-135b has a neuroprotective role via targeting of β-site APP-cleaving enzyme 1. Experimental and Therapeutic Medicine, 12, 809-814. https://doi.org/10.3892/etm.2016.3366
MLA
Zhang, Y., Xing, H., Guo, S., Zheng, Z., Wang, H., Xu, D."MicroRNA-135b has a neuroprotective role via targeting of β-site APP-cleaving enzyme 1". Experimental and Therapeutic Medicine 12.2 (2016): 809-814.
Chicago
Zhang, Y., Xing, H., Guo, S., Zheng, Z., Wang, H., Xu, D."MicroRNA-135b has a neuroprotective role via targeting of β-site APP-cleaving enzyme 1". Experimental and Therapeutic Medicine 12, no. 2 (2016): 809-814. https://doi.org/10.3892/etm.2016.3366
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team