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Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice

  • Authors:
    • Li Yuan
    • Hou‑Qi Liu
    • Min‑Juan Wu
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University, Shanghai 200433, P.R. China
    Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 641-648
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    Published online on: May 24, 2016
       https://doi.org/10.3892/etm.2016.3383
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Abstract

Stem cells are used with increasing success in the treatment of renal tubular injury. However, whether mesenchymal stem cells (MSC) differentiate into renal tubular epithelial cells remains controversial. The aims of the present study were to observe the localization of human embryonic MSCs (hMSCs) in the kidneys of newborn mice, and to investigate hMSC differentiation into tubular epithelium. Primary culture hMSCs were derived from 4‑7‑week‑old embryos and labeled with the cell membrane fluorescent dye PKH‑26. The degree of apoptosis, cell growth, differentiation and localization of hMSCs with and without this label were then determined using immunohistochemical methods and flow cytometry. hMSCs and PKH26‑labeled hMSCs were revealed to differentiate into chondrocytes and adipocytes, and were demonstrated to have similar proliferative capability. In the two cell types, the antigens CD34 and CD45, indicative of hematopoietic lineages, were not expressed; however, the expression of the mesenchymal markers CD29 and CD90 in MSCs, was significantly increased. During a 4‑week culture period, laser confocal microscopy revealed that PKH26‑labeled hMSCs in the kidneys of newborn mice gradually dispersed. Two weeks after the injection of the PKH26‑labeled cells, the percentage of PKH26‑labeled hMSCs localized to the renal tubules was 10±2.1%. In conclusion, PKH26 labeling has no effect on hMSC differentiation, proliferation and mesenchymal cell surface features, and hMSCs injected into the kidneys of newborn mice may transform to renal tubule epithelium.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan L, Liu HQ and Wu MJ: Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice. Exp Ther Med 12: 641-648, 2016.
APA
Yuan, L., Liu, H., & Wu, M. (2016). Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice. Experimental and Therapeutic Medicine, 12, 641-648. https://doi.org/10.3892/etm.2016.3383
MLA
Yuan, L., Liu, H., Wu, M."Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice". Experimental and Therapeutic Medicine 12.2 (2016): 641-648.
Chicago
Yuan, L., Liu, H., Wu, M."Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice". Experimental and Therapeutic Medicine 12, no. 2 (2016): 641-648. https://doi.org/10.3892/etm.2016.3383
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan L, Liu HQ and Wu MJ: Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice. Exp Ther Med 12: 641-648, 2016.
APA
Yuan, L., Liu, H., & Wu, M. (2016). Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice. Experimental and Therapeutic Medicine, 12, 641-648. https://doi.org/10.3892/etm.2016.3383
MLA
Yuan, L., Liu, H., Wu, M."Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice". Experimental and Therapeutic Medicine 12.2 (2016): 641-648.
Chicago
Yuan, L., Liu, H., Wu, M."Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice". Experimental and Therapeutic Medicine 12, no. 2 (2016): 641-648. https://doi.org/10.3892/etm.2016.3383
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