Benexate hydrochloride betadex modulates nitric oxide synthesis and cytokine expression in gastric ulcers

Lee,Jae  Min; Lim,Ji‑Youn; Kim,Yoonjin; Kim,Ye  Ji; Choi,Hyuk  Soon; Kim,Eun  Sun; Keum,Bora; Seo,Yeon  Seok; Jeen,Yoon  Tae; Lee,Hong  Sik; Um,Soon  Ho; Kim,Chang  Duck; Ryu,Ho  Sang; Sul,Donggeun; Hong,Junghwa; Chun,Hoon  Jai

doi:10.3892/etm.2016.3384

August-2016 Volume 12 Issue 2

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August-2016 Volume 12 Issue 2

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Article Open Access

Benexate hydrochloride betadex modulates nitric oxide synthesis and cytokine expression in gastric ulcers

Authors:
- Jae Min Lee
- Ji‑Youn Lim
- Yoonjin Kim
- Ye Ji Kim
- Hyuk Soon Choi
- Eun Sun Kim
- Bora Keum
- Yeon Seok Seo
- Yoon Tae Jeen
- Hong Sik Lee
- Soon Ho Um
- Chang Duck Kim
- Ho Sang Ryu
- Donggeun Sul
- Junghwa Hong
- Hoon Jai Chun
View Affiliations / Copyright

Affiliations: Division of Gastroenterology and Hepatology, Department of Internal Medicine, College of Medicine, Korea University, Seoul 02841, Republic of Korea, Graduate School of Medicine, Korea University, Seoul 02841, Republic of Korea, Department of Control and Instrumentation Engineering, Korea University, Sejong 30019, Republic of Korea

Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 573-580
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Published online on: May 24, 2016

https://doi.org/10.3892/etm.2016.3384
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Abstract

The present study investigated benexate hydrochloride betadex (BHB)-mediated ulcer healing, and changes to microcirculation modulated through nitric oxide synthase (NOS) and anti‑inflammatory activity. A rat model of gastric mucosal injury was established through injection of a 60% acetic acid solution into the stomach. Following ulcer induction, the rats were administered BHB orally for 5 days at doses of 0, 100, 300 or 1,000 mg/kg. The highest dose of BHB was also administered with or without L-NG-nitroarginine methyl ester (L‑NAME). The area of gastric ulcers was determined by planimetry, and expression of cyclooxygenases (COX), cytokines and NOS in stomach tissues were measured using western blotting. Compared with the control group, gastric ulcer size was significantly decreased in the 1,000 mg/kg BHB‑treated group (P<0.05). Administration of BHB led to a significant increase in endothelial (e)NOS expression (P<0.05). Although acetic acid co‑treatment with L‑NAME induced more severe mucosal damage, BHB decreased COX expression and tumor necrosis factor‑α levels when administered with the nitric oxide inhibitor, L-NAME (P<0.05). BHB exhibited protective effects in a rat model of gastric ulcers, which were associated with a decrease in pro‑inflammatory cytokine levels and the activation of eNOS.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Benexate hydrochloride betadex modulates nitric oxide synthesis and cytokine expression in gastric ulcers

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